An organotypic model of skin to study photodamage and photoprotection in vitro
Abstract
Acute or repetitive sun exposures are known to elicit cutaneous damages such as sunburn but also long-term effects such as photoaging or cancers. Determination of early biological events occurring after ultraviolet (UV) exposure is essential for photoprotection. Using skin reconstructed in vitro containing both a dermal equivalent and a fully differentiated epidermis, the effects of UV light (UVB and UVA) were investigated. UVB-induced damage was essentially epidermal, with the typical sunburn cells and DNA lesions, whereas UVA radiation-induced damage was mostly located within the dermal compartment. The model and end points used for UVB- and UVA-induced damages appeared to be very useful for the in vitro evaluation of sunscreens after topical application, in particular to investigate its protective effects against the effects of UVR, and allowed us to distinguish the efficiency of absorbers depending on their absorption spectrum.
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UV-A accelerates hydrolysis of skin collagen by promoting production of matrix metalloproteinases, leading to tissue destruction and progressive degeneration of dermal extracellular matrix.
“It accelerates the hydrolysis of skin collagen by promoting the production of matrix metalloproteinases (MMPs), leading to tissue destruction and progressive degeneration of dermal extracellular matrix [22,23,24].”
From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [24]
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Asselineau, D., Ricois, S., Pageon, H., Zucchi, H., Girardeau‐Hubert, S., Deneuville, C., Haydont, V., Neiveyans, V., & Lorthois, I. (2015) · Springer eBooks · doi:10.1007/978-3-642-27814-3_48-2
Self-citationIn Vitro Method to Visualize UV-Induced Reactive Oxygen Species in a Skin Equivalent Model
Hakozaki, T. (2015) · Springer eBooks · doi:10.1007/978-3-642-27814-3_49-2
In Vitro Method to Visualize UV-Induced Reactive Oxygen Species in a Skin Equivalent Model
Hakozaki, T. (2015) · Springer eBooks · doi:10.1007/978-3-642-27814-3_49-3
In Vitro Method to Visualize UV-Induced Reactive Oxygen Species in a Skin Equivalent Model
Hakozaki, T. (2016) · Springer eBooks · doi:10.1007/978-3-662-47398-6_49
A Bioengineered Skin Organoid Platform for Modeling Human Skin Physiology and CytotoxicityPreprint
Gorkun, A. A., Mahajan, N., Nomdedeu‐Sancho, G., Jorgensen, A. M., Willson, K., Wu, M., Ji, D., Söker, S., & Atala, A. (2025) · Research Square · doi:10.21203/rs.3.rs-7989400/v1
Skincare for All Ages: The Science, Advances and Future of Skincare from Infancy to Adulthood
Echeverry, A. (2026) · AAPS introductions in the pharmaceutical sciences · doi:10.1007/978-3-032-03596-7_3
Current and emerging new approach & methodologies for skin hazard assessment
Shin, S.-Y., & Lim, K. (2026) · Toxicological Research · doi:10.1007/s43188-026-00374-8
Mechanisms of Skin Photoaging and Advances in Active Ingredient-Based Interventions: A Review
Qiu, C., 杜克斯, Zhao, F., Zheng, S., & Zheng, H. (2026) · Journal of Dermatologic Science and Cosmetic Technology · doi:10.1016/j.jdsct.2026.100198
- Authors
- Bernerd, F., & Asselineau, D.
- Affiliations
- L'Oréal (France) (FR)
- Corresponding author
- Bernerd, Françoise
- ORCID
- Bernerd, Françoise
- Venue
- Journal of the American Academy of Dermatology
- Publisher
- Elsevier BV
- Published
- 2008-05
- Volume
- 58
- Issue
- 5
- Pages
- S155-S159
- ISSN
- 0190-9622 (ISSN-L)
- OpenAlex
- W2045702642
- Cited by
- 86
- References
- 27
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 4 · 2023: 8 · 2022: 4 · 2021: 1 · 2020: 5 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 9 · 2014: 5 · 2013: 6 · 2012: 5
- FWCI
- 4.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Academy of Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
- Health Sciences › Medicine › Epidemiology › Nonmelanoma Skin Cancer Studies
- Keywords
- organotypic skin model, reconstructed skin, photodamage, photoprotection, ultraviolet radiation, UV-B radiation, UVA, sunburn, photoaging, sunscreen, dermal equivalent, epidermis, DNA damage
- MeSH terms
- Cells, Cultured, Humans, Skin, Sunscreening Agents, Ultraviolet Rays, Skin Aging, Keratinocytes, Skin, Artificial, In Vitro Techniques
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