Cited by 86Skin Protection and Agingmelanin and skin pigmentationNonmelanoma Skin Cancer Studies

An organotypic model of skin to study photodamage and photoprotection in vitro

Bernerd, Françoise, Asselineau, Daniel·2008·Journal of the American Academy of Dermatology

Abstract

Acute or repetitive sun exposures are known to elicit cutaneous damages such as sunburn but also long-term effects such as photoaging or cancers. Determination of early biological events occurring after ultraviolet (UV) exposure is essential for photoprotection. Using skin reconstructed in vitro containing both a dermal equivalent and a fully differentiated epidermis, the effects of UV light (UVB and UVA) were investigated. UVB-induced damage was essentially epidermal, with the typical sunburn cells and DNA lesions, whereas UVA radiation-induced damage was mostly located within the dermal compartment. The model and end points used for UVB- and UVA-induced damages appeared to be very useful for the in vitro evaluation of sunscreens after topical application, in particular to investigate its protective effects against the effects of UVR, and allowed us to distinguish the efficiency of absorbers depending on their absorption spectrum.

Integrity signals

Registry

Crossref · 10.1016/j.jaad.2007.08.050

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27 of 27 references verified0 citation errors · 0 not checkable

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  • UV-A accelerates hydrolysis of skin collagen by promoting production of matrix metalloproteinases, leading to tissue destruction and progressive degeneration of dermal extracellular matrix.

    “It accelerates the hydrolysis of skin collagen by promoting the production of matrix metalloproteinases (MMPs), leading to tissue destruction and progressive degeneration of dermal extracellular matrix [22,23,24].”

    From Diet and Skin Aging—From the Perspective of Food Nutrition (2020) · cites this paper as [24]

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Authors
Bernerd, F., & Asselineau, D.
Affiliations
L'Oréal (France) (FR)
Corresponding author
Bernerd, Françoise
Venue
Journal of the American Academy of Dermatology
Publisher
Elsevier BV
Published
2008-05
Volume
58
Issue
5
Pages
S155-S159
ISSN
0190-9622 (ISSN-L)
OpenAlex
W2045702642
Cited by
86
References
27
Citations by year
2026: 4 · 2025: 4 · 2024: 4 · 2023: 8 · 2022: 4 · 2021: 1 · 2020: 5 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 6 · 2015: 9 · 2014: 5 · 2013: 6 · 2012: 5
FWCI
4.29
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of the American Academy of Dermatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Dermatology › Skin Protection and Aging
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › melanin and skin pigmentation
  • Health Sciences › Medicine › Epidemiology › Nonmelanoma Skin Cancer Studies
Keywords
organotypic skin model, reconstructed skin, photodamage, photoprotection, ultraviolet radiation, UV-B radiation, UVA, sunburn, photoaging, sunscreen, dermal equivalent, epidermis, DNA damage
MeSH terms
Cells, Cultured, Humans, Skin, Sunscreening Agents, Ultraviolet Rays, Skin Aging, Keratinocytes, Skin, Artificial, In Vitro Techniques

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