Cited by 36Epigenetics and DNA MethylationSuicide and Self-Harm StudiesGenetic Associations and Epidemiology

Genome-wide DNA methylation in suicide victims revealing impact on gene expression

Kouter, Katarina, Zupanc, Tomaž, Videtič Paska, Alja·2019·Journal of Affective Disorders

Abstract

Background Suicidal behavior is a multifactorial, polygenic state that affects millions worldwide. It is a result of interplay between hereditary and environmental factors, tied together by epigenetics. Despite vast knowledge on suicidality complete mechanism and factors leading to suicide are unknown. However there is an indication between changes in DNA methylation patterns and suicidal behavior.Methods To identify differential methylation we formed a homogenous group of male suicide victims who died by hanging and control group. Altogether our study included 18 subjects in which two brain regions, Brodmann area 9 (9 suicide victims and 9 controls)) and hippocampus (6 suicide victims and 6 controls) were investigated using next-generation sequencing (NGS).Results Our results have shown several differences in methylation level between suicide victims and controls in both brain regions (>25% difference in methylation and q-value < 0.01), with gene ontology pointing towards cell structural integrity and nervous system regulation. Additional gene expression analysis identified changes in two genes, ZNF714 (p-value = 0.002) and NRIP3 (p-value = 0.046).Limitations Major limitation is small sample size. Our analysis was conducted on brain tissue including different cell types so the results are a representation of a methylation pattern for the whole brain tissue sample.Conclusions We performed a preliminary methylation study with single base pair resolution using NGS on one of the world populations with a very high suicide risk. Obtained results offer novel insights into altered methylation patterns in suicide victims, which could provide a starting point for further studies on clinical samples with highly expressed suicide risk.

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Crossref · 10.1016/j.jad.2019.04.077

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Authors
Kouter, K., Zupanc, T., & Videtič Paska, A.
Affiliations
University of Ljubljana (SI)
Venue
Journal of Affective Disorders
Publisher
Elsevier BV
Published
2019-06
Volume
253
Pages
419-425
ISSN
0165-0327 (ISSN-L)
OpenAlex
W2938845993
Cited by
36
References
32
Citations by year
2026: 4 · 2025: 6 · 2024: 4 · 2023: 5 · 2022: 4 · 2021: 7 · 2020: 4 · 2019: 2
FWCI
1.41
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Affective Disorders (publishedVersion)
  • PubMed (publishedVersion)
Funders
Slovenian Research Agency (P1-0104, J3-7132)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Social Sciences › Psychology › Clinical Psychology › Suicide and Self-Harm Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Associations and Epidemiology
Keywords
suicide, DNA methylation, epigenetics, gene expression, hanging, Brodmann area 9, hippocampus, brain tissue, suicide risk
MeSH terms
Adult, Brain, Cerebral Cortex, DNA, DNA-Binding Proteins, Female, Hippocampus, Humans, Male, Promoter Regions, Genetic, Suicide, Temporal Lobe, Transcription Factors, Gene Expression, Self-Injurious Behavior, DNA Methylation, Epigenesis, Genetic, Epigenomics, Suicidal Ideation

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