Cited by 110Polysaccharides Composition and ApplicationsProbiotics and Fermented FoodsBiopolymer Synthesis and Applications

Preparation, Partial Characterization and Biological Activity of Exopolysaccharides Produced from Lactobacillus fermentum S1

Wang, Kun, Niu, Mengmeng, Song, Dawei, Song, Xuejian, Zhao, Jing, Wu, Yue, Lu, Baoxin, Niu, Guangcai·2020·Journal of Bioscience and Bioengineering

Abstract

A high slime-producing Lactobacillus fermentum strain (named as L. fermentum S1) was isolated from traditional fermented Fuyuan pickle, which was made of white turnip and collected from Fuyuan county, Yunnan province, China. We extracted and purified the exopolysaccharides from L. fermentum S1, and investigated their preliminary structure characteristics and biological activities. Three purified exopolysaccharide fractions, designated as EPS1, EPS2 and EPS3, were obtained from the culture supernatant of L. fermentum S1 by ethanol precipitation, anion exchange and gel filtration chromatography. The EPS2 and EPS3 were homogeneous with molecular weights of 4.45 × 10 6 and 2.82 × 10 6 Da, respectively. All the purified EPS fractions were composed of glucose, galactose, mannose and arabinose, but with different molar ratios. EPS1, EPS2 and EPS3 presented different surface morphologies and their degradation temperatures were 302.7°C, 316.3°C and 316.9°C, respectively. Bioactivity research showed that L. fermentum S1 EPS elicited free radical scavenging capacity and ferric reducing antioxidant power, and 1 mg/mL of EPS significantly improved the gastrointestinal transit tolerance of non EPS-producing strain L. fermentum LG1. Moreover, S1 EPS had a favorable anti-biofilm activity against Escherichia coli and Staphylococcus aureus. These results indicated that S1 EPS could be explored as a promising functional adjunct for application in foods.

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Crossref · 10.1016/j.jbiosc.2019.07.009

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  • The health functionality of EPS is correlated with specific molecular and structural properties, such as molecular weight, alignment and monosaccharide composition, that affect their bioactivity.

    “The health functionality of EPS is correlated with the specific molecular and structural properties (e.g., molecular weight, alignment and monosaccharide composition) that are affecting their bioactivity [109].”
  • Some EPS produced from different LAB species with antioxidant activity have also shown immunomodulatory and anti-inflammatory properties, suggesting a potential correlation.

    “some EPS produced from different LAB species with antioxidant activity have also shown the ability to exert other beneficial effects such as immunomodulatory and anti-inflammatory properties, suggesting a potential correlation [109,119,124,125,126].”

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Authors
Wang, K., Niu, M., Song, D., Song, X., Zhao, J., Wu, Y., Lu, B., & Niu, G.
Affiliations
Heilongjiang Bayi Agricultural University (CN)
Venue
Journal of Bioscience and Bioengineering
Publisher
Elsevier BV
Published
2020-02
Volume
129
Issue
2
Pages
206-214
ISSN
  • 1347-4421 (ISSN-L)
  • 1389-1723
OpenAlex
W2970220052
Cited by
110
References
43
Citations by year
2026: 11 · 2025: 24 · 2024: 24 · 2023: 21 · 2022: 13 · 2021: 8 · 2020: 9
FWCI
5.79
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Bioscience and Bioengineering (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Natural Science Foundation of Heilongjiang Province (QC2016032)
  • Heilongjiang BaYi Agricultural University (CXRC2016-10, 2016-KYYWF-0170)
  • University Nursing Program of Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2018082)
  • Agricultural Science and Technology Park (ZY18B01)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
Keywords
exopolysaccharides, Lactobacillus fermentum, polysaccharide purification, polysaccharide structure, antioxidant activity, antibiofilm activity, Escherichia coli, Staphylococcus aureus, functional food additives
MeSH terms
Antioxidants, China, Fermentation, Molecular Weight, Polysaccharides, Bacterial, Limosilactobacillus fermentum

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