Preparation, Partial Characterization and Biological Activity of Exopolysaccharides Produced from Lactobacillus fermentum S1
Abstract
A high slime-producing Lactobacillus fermentum strain (named as L. fermentum S1) was isolated from traditional fermented Fuyuan pickle, which was made of white turnip and collected from Fuyuan county, Yunnan province, China. We extracted and purified the exopolysaccharides from L. fermentum S1, and investigated their preliminary structure characteristics and biological activities. Three purified exopolysaccharide fractions, designated as EPS1, EPS2 and EPS3, were obtained from the culture supernatant of L. fermentum S1 by ethanol precipitation, anion exchange and gel filtration chromatography. The EPS2 and EPS3 were homogeneous with molecular weights of 4.45 × 10 6 and 2.82 × 10 6 Da, respectively. All the purified EPS fractions were composed of glucose, galactose, mannose and arabinose, but with different molar ratios. EPS1, EPS2 and EPS3 presented different surface morphologies and their degradation temperatures were 302.7°C, 316.3°C and 316.9°C, respectively. Bioactivity research showed that L. fermentum S1 EPS elicited free radical scavenging capacity and ferric reducing antioxidant power, and 1 mg/mL of EPS significantly improved the gastrointestinal transit tolerance of non EPS-producing strain L. fermentum LG1. Moreover, S1 EPS had a favorable anti-biofilm activity against Escherichia coli and Staphylococcus aureus. These results indicated that S1 EPS could be explored as a promising functional adjunct for application in foods.
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The health functionality of EPS is correlated with specific molecular and structural properties, such as molecular weight, alignment and monosaccharide composition, that affect their bioactivity.
“The health functionality of EPS is correlated with the specific molecular and structural properties (e.g., molecular weight, alignment and monosaccharide composition) that are affecting their bioactivity [109].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [109]
Some EPS produced from different LAB species with antioxidant activity have also shown immunomodulatory and anti-inflammatory properties, suggesting a potential correlation.
“some EPS produced from different LAB species with antioxidant activity have also shown the ability to exert other beneficial effects such as immunomodulatory and anti-inflammatory properties, suggesting a potential correlation [109,119,124,125,126].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [109]
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- Authors
- Wang, K., Niu, M., Song, D., Song, X., Zhao, J., Wu, Y., Lu, B., & Niu, G.
- Affiliations
- Heilongjiang Bayi Agricultural University (CN)
- Venue
- Journal of Bioscience and Bioengineering
- Publisher
- Elsevier BV
- Published
- 2020-02
- Volume
- 129
- Issue
- 2
- Pages
- 206-214
- ISSN
- 1347-4421 (ISSN-L)
- 1389-1723
- OpenAlex
- W2970220052
- Cited by
- 110
- References
- 43
- Citations by year
- 2026: 11 · 2025: 24 · 2024: 24 · 2023: 21 · 2022: 13 · 2021: 8 · 2020: 9
- FWCI
- 5.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Bioscience and Bioengineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation of Heilongjiang Province (QC2016032)
- Heilongjiang BaYi Agricultural University (CXRC2016-10, 2016-KYYWF-0170)
- University Nursing Program of Young Scholars with Creative Talents in Heilongjiang Province (UNPYSCT-2018082)
- Agricultural Science and Technology Park (ZY18B01)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biopolymer Synthesis and Applications
- Keywords
- exopolysaccharides, Lactobacillus fermentum, polysaccharide purification, polysaccharide structure, antioxidant activity, antibiofilm activity, Escherichia coli, Staphylococcus aureus, functional food additives
- MeSH terms
- Antioxidants, China, Fermentation, Molecular Weight, Polysaccharides, Bacterial, Limosilactobacillus fermentum
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