In vitro biological activity and essential oil composition of four indigenous South African Helichrysum species
Abstract
Helichrysum species are used widely to treat various medical conditions. In this study, the anti-microbial, anti-oxidant (DPPH assay) and anti-inflammatory activity (5-lipoxygenase assay) of Helichrysum dasyanthum, Helichrysum felinum, Helichrysum excisum and Helichrysum petiolare were investigated. The essential oil compositions of these species were determined. The acetone and methanol extracts as well as the essential oils exhibited activity against Gram-positive bacteria, while both the methanol and acetone extracts of all four species were active in the anti-oxidant assay. The essential oils, on the other hand, displayed activity in the 5-lipoxygenase assay, which was used as an indication of anti-inflammatory activity. Two extracts exhibited promising activity in the anti-microbial assay, the acetone extract of Helichrysum dasyanthum with a MIC value of 15.63 microg/ml and the methanol extract of Helichrysum excisum with a MIC value of 62.5 microg/ml. The acetone extract of Helichrysum dasyanthum was the most active free radical scavenger in the DPPH assay (IC(50) of 9.53 microg/ml) while values for the anti-inflammatory activity of the essential oils ranged between 25 and 32 microg/ml. The essential oil compositions of three species (Helichrysum dasyanthum, Helichrysum excisum and Helichrysum petiolare) were dominated by the presence of monoterpenes such as alpha-pinene, 1,8-cineole and p-cymene. In the oil of Helichrysum felinum, monoterpenes were largely absent. Its profile consisted of a variety of sesquiterpenes in low concentrations with beta-caryophyllene dominating.
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The same major components present in essential oils of four indigenous South African Helichrysum species inhibit 5-lipoxygenase.
“The same major components present in the essential oils of four indigenous South African Helichrysum species were also reported to inhibit 5-lipoxygenase [99].”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [99]
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- Authors
- Lourens, A. C. U., Reddy, D., Başer, K. H. C., Viljoen, A. M., & Van Vuuren, S. F.
- Affiliations
- University of the Witwatersrand (ZA)
- Anadolu University (TR)
- Corresponding author
- Lourens, A.C.U.
- Venue
- Journal of Ethnopharmacology
- Publisher
- Elsevier BV
- Published
- 2004-12
- Volume
- 95
- Issue
- 2-3
- Pages
- 253-258
- ISSN
- 0378-8741 (ISSN-L)
- OpenAlex
- W1976538578
- Cited by
- 196
- References
- 27
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 4 · 2023: 6 · 2022: 11 · 2021: 13 · 2020: 15 · 2019: 10 · 2018: 19 · 2017: 11 · 2016: 9 · 2015: 8 · 2014: 7 · 2013: 11 · 2012: 8
- FWCI
- 7.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Ethnopharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- Helichrysum, Helichrysum dasyanthum, Helichrysum felinum, Helichrysum petiolare, essential oil composition, antimicrobial activity, antioxidant activity, anti-inflammatory activity, 5-lipoxygenase inhibition, monoterpenes, sesquiterpenes, β-caryophyllene, Gram-positive bacteria, medicinal plants
- MeSH terms
- Microbial Sensitivity Tests, Oils, Volatile, Plant Extracts, South Africa, Medicine, African Traditional, Plant Structures, Helichrysum
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