In vitro efficacy of liposomal amphotericin B, micafungin and fluconazole against non-albicans Candida species biofilms
Abstract
Non-albicans Candida species are being isolated with increasing frequency. In this study, biofilm formation by Candida tropicalis, Candida parapsilosis and Candida glabrata was evaluated and the activities of liposomal amphotericin B (LAB), micafungin (MFG) and fluconazole (FLC) against these biofilms were assessed using a clinically relevant in vitro model system. LAB exhibited strong activities against the three non-albicans Candida species and showed dose-dependent efficacy. MFG displayed a paradoxical growth effect against the C. tropicalis biofilm. FLC was ineffective for non-albicans biofilms. This study shows that Candida biofilms have unique susceptibility to LAB. The dose-dependent effects of LAB indicate that this drug may be a useful treatment for biofilm formation by non-albicans Candida species in cases in which the catheter cannot be removed for clinical reasons.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Both C. albicans and C. glabrata can form biofilms on abiotic substrates, especially medical devices including catheters and implanted materials.
“Both C. albicans and C. glabrata can form biofilms on abiotic substrates, especially medical devices including catheters and implanted materials [26,27].”
From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [26]
Cites
Clinical and Laboratory Standards Institute
Gressner, A. M., & Arndt, T. (2019) · Springer Reference Medizin · doi:10.1007/978-3-662-48986-4_300416 · Cited by 5,092 · see citing works
Candida biofilms and their role in infection
Douglas, L. J. (2003) · Trends in Microbiology · doi:10.1016/s0966-842x(02)00002-1 · Cited by 1,150 · see citing works
Reference method for broth dilution antifungal susceptibility testing of yeasts : Approved standard
Rex, J. H., & Clinical (2008) · Clinical and Laboratory Standards Institute eBooks · Cited by 1,052 · see citing works
Candida parapsilosis , an Emerging Fungal Pathogen
Trofa, D. P., Gácser, A., & Nosanchuk, J. D. (2008) · Clinical Microbiology Reviews · doi:10.1128/cmr.00013-08 · Cited by 862 · see citing works
Andes, D. R., Safdar, N., Baddley, J. W., Playford, G., Reboli, A. C., Rex, J. H., Sobel, J. D., Pappas, P. G., Kullberg, B. J., & for the Mycoses Study Group (2012) · Clinical Infectious Diseases · doi:10.1093/cid/cis021 · Cited by 793 · see citing works
Kuhn, D. M., George, T., Chandra, J., Mukherjee, P. K., & Ghannoum, M. A. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.6.1773-1780.2002 · Cited by 765 · see citing works
Ramage, G., Vande Walle, K., Wickes, B. L., & López-Ribot, J. L. (2001) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.45.9.2475-2479.2001 · Cited by 753 · see citing works
<i>Candida</i> biofilms on implanted biomaterials: a clinically significant problem
Ramage, G., MartÃnez, J. P., & López-Ribot, J. L. (2006) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2006.00117.x · Cited by 575 · see citing works
Comparison of Biofilms Formed byCandidaalbicansandCandidaparapsilosison Bioprosthetic Surfaces
Kuhn, D. M., Chandra, J., Mukherjee, P. K., & Ghannoum, M. A. (2002) · Infection and Immunity · doi:10.1128/iai.70.2.878-888.2002 · Cited by 485 · see citing works
Pfaller, M. A., Neofytos, D., Diekema, D. J., Azie, N. E., Meier‐Kriesche, H., Quan, S.-P., & Horn, D. (2012) · Diagnostic Microbiology and Infectious Disease · doi:10.1016/j.diagmicrobio.2012.10.003 · Cited by 391 · see citing works
Tumbarello, M., Posteraro, B., Trecarichi, E. M., Fiori, B., Rossi, M., Porta, R., de Gaetano Donati, K., La Sorda, M., Spanu, T., Fadda, G., et al. (2007) · Journal of Clinical Microbiology · doi:10.1128/jcm.00131-07 · Cited by 343 · see citing works
Pfaller, M. A., Andes, D., Diekema, D. J., Espinel-Ingroff, A., & Sheehan, D. (2010) · Drug Resistance Updates · doi:10.1016/j.drup.2010.09.002 · Cited by 314 · see citing works
Adherence and biofilm formation of non-Candida albicans Candida species
Silva, S., Negri, M., Henriques, M., Oliveira, R., Williams, D. W., & Azeredo, J. (2011) · Trends in Microbiology · doi:10.1016/j.tim.2011.02.003 · Cited by 304 · see citing works
In Vitro Activity of Caspofungin against <i>Candida albicans</i> Biofilms
Bachmann, S. P., VandeWalle, K., Ramage, G., Patterson, T. F., Wickes, B. L., Graybill, J. R., & López-Ribot, J. L. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.11.3591-3596.2002 · Cited by 288 · see citing works
Ramage, G., VandeWalle, K., Bachmann, S. P., Wickes, B. L., & Lopez-Ribot, J. L. (2002) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.46.11.3634-3636.2002 · Cited by 238 · see citing works
Stevens, D. A., Espiritu, M., & Parmar, R. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.9.3407-3411.2004 · Cited by 218 · see citing works
Kumamoto, C. A. (2002) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(02)00371-5 · Cited by 217 · see citing works
Elevated Chitin Content Reduces the Susceptibility of Candida Species to Caspofungin
Walker, L. A., Gow, N. A. R., & Munro, C. A. (2013) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01486-12 · Cited by 214 · see citing works
Tumbarello, M., Fiori, B., Trecarichi, E. M., Posteraro, P., Losito, A. R., Luca, A. D., Sanguinetti, M., Fadda, G., Cauda, R., & Posteraro, B. (2012) · PLoS ONE · doi:10.1371/journal.pone.0033705 · Cited by 197 · see citing works
Rabbit Model of Candida albicans Biofilm Infection: Liposomal Amphotericin B Antifungal Lock Therapy
Schinabeck, M. K., Long, L. A., Hossain, M. A., Chandra, J., Mukherjee, P. K., Mohamed, S., & Ghannoum, M. A. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.5.1727-1732.2004 · Cited by 180 · see citing works
Chamilos, G., Lewis, R. E., Albert, N., & Kontoyiannis, D. P. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00095-07 · Cited by 171 · see citing works
Characteristics of biofilm formation by Candida tropicalis and antifungal resistance
Bizerra, F. C., Nakamura, C. V., Poersch, C. D. O., Svidzinski, T. I. E., Quesada, R. M. B., Goldenberg, S., Krieger, M. A., & Yamada‐Ogatta, S. F. (2008) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2007.00347.x · Cited by 170 · see citing works
Candida biofilms: antifungal resistance and emerging therapeutic options.
Kuhn, D. M., & Ghannoum, M. A. (2004) · PubMed · Cited by 166 · see citing works
Hawser, S., Norris, H. A., Jessup, C. J., & Ghannoum, M. A. (1998) · Journal of Clinical Microbiology · doi:10.1128/jcm.36.5.1450-1452.1998 · Cited by 140 · see citing works
Takakura, S. (2004) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkh053 · Cited by 117 · see citing works
Melo, A. S. D. A., Colombo, A. L., & Arthington, B. A. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00676-07 · Cited by 117 · see citing works
Katragkou, A., McCarthy, M. W., Alexander, E. L., Antachopoulos, C., Meletiadis, J., Jabra‐Rizk, M. A., Petraitis, V., Roilides, E., & Walsh, T. J. (2014) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dku374 · Cited by 111 · see citing works
Serum Differentially Alters the Antifungal Properties of Echinocandin Drugs
Paderu, P., García‐Effrón, G., Balashov, S. V., Delmas, G., Park, S., & Perlin, D. S. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01536-06 · Cited by 109 · see citing works
Shuford, J. A., Rouse, M. S., Piper, K. E., Steckelberg, J. M., & Patel, R. (2006) · The Journal of Infectious Diseases · doi:10.1086/506452 · Cited by 100 · see citing works
Uppuluri, P., Srinivasan, A., Ramasubramanian, A. K., & Lopez-Ribot, J. L. (2011) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01701-10 · Cited by 99 · see citing works
Choi, H. W., Shin, J. H., Jung, S., Park, K. H., Cho, D., Kee, S., Shin, M., Suh, S., & Ryang, D. W. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01141-06 · Cited by 85 · see citing works
Ferreira, J. A., Carr, J. H., Starling, C. E. F., de Resende, M. A., & Donlan, R. M. (2009) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00316-09 · Cited by 84 · see citing works
Emerging trends in candidemia: A higher incidence but a similar outcome
Fortün, J., Martín‐Dávila, P., de la Pedrosa, E. G., Pintado, V., Cobo, J., Fresco, G., Meije, Y., Ros, L., Álvarez, M. E. D., Luengo, J. D. M. L., et al. (2012) · Journal of Infection · doi:10.1016/j.jinf.2012.02.011 · Cited by 75 · see citing works
Effects of Serum on In Vitro Susceptibility Testing of Echinocandins
Odabaşı, Z., Paetznick, V. L., Rex, J. H., & Ostrosky‐Zeichner, L. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01589-06 · Cited by 69 · see citing works
Nagao, M. (2012) · Clinical Microbiology and Infection · doi:10.1111/1469-0691.12083 · Cited by 68 · see citing works
In Vitro Activity of Anidulafungin against Candida albicans Biofilms
Jacobson, M. J., Piper, K. E., Nguyen, G., Steckelberg, J. M., & Patel, R. (2008) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00211-08 · Cited by 45 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival
Hassan, Y., Chew, S. Y., & Than, L. T. L. (2021) · Journal of Fungi · doi:10.3390/jof7080667 · Cited by 170 · see citing works
Biofilms and beyond: Expanding echinocandin utility
Larkin, E. L., Dharmaiah, S., & Ghannoum, M. A. (2018) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkx451 · Cited by 31 · see citing works
- Authors
- Kawai, A., Yamagishi, Y., & Mikamo, H.
- Affiliations
- Aichi Medical University (JP)
- Corresponding author
- Mikamo, Hiroshige
- Venue
- Journal of Infection and Chemotherapy
- Publisher
- Elsevier BV
- Published
- 2015-09
- Volume
- 21
- Issue
- 9
- Pages
- 647-653
- ISSN
- 1341-321X (ISSN-L)
- OpenAlex
- W1903085608
- Cited by
- 23
- References
- 36
- Citations by year
- 2025: 1 · 2023: 2 · 2022: 4 · 2021: 5 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 5 · 2016: 1
- FWCI
- 2.33
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Infection and Chemotherapy (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- non-albicans Candida, Candida biofilms, liposomal amphotericin B, micafungin, fluconazole, Candida tropicalis, Candida parapsilosis, Candida glabrata, antifungal susceptibility
- MeSH terms
- Micafungin, Amphotericin B, Antifungal Agents, Microbial Sensitivity Tests, Fluconazole, Biofilms, Candida tropicalis, Candida glabrata, Echinocandins, Lipopeptides, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review