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Exploring cellular biochemistry with nanobodies

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Fluonanobody-based nanosensor via fluorescence resonance energy transfer for ultrasensitive detection of ochratoxin A

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Nanobody-Targeted Photodynamic Therapy Selectively Kills Viral GPCR-Expressing Glioblastoma Cells

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Nanobodies: Robust miniprotein binders in biomedicine

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Application of Single-Domain Antibodies (“Nanobodies”) to Laboratory Diagnosis

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Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies

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Research progress and applications of nanobody in human infectious diseases

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Nanobody Conjugates for Targeted Cancer Therapy and Imaging

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COMBINES-CID: An Efficient Method for De Novo Engineering of Highly Specific Chemically Induced Protein Dimerization Systems

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Computational design and modeling of nanobodies toward SARS‐CoV‐2 receptor binding domain

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Homology Modeling-Based in Silico Affinity Maturation Improves the Affinity of a Nanobody

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Nanobodies: The Future of Antibody-Based Immune Therapeutics

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Targeted drug delivery using nanobodies to deliver effective molecules to breast cancer cells: the most attractive application of nanobodies

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Structural and thermodynamic basis for the recognition of the substrate-binding cleft on hen egg lysozyme by a single-domain antibody

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Prospects for the computational humanization of antibodies and nanobodies

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Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays

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Nanobodies: From High-Throughput Identification to Therapeutic Development

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Importance and Considerations of Antibody Engineering in Antibody-Drug Conjugates Development from a Clinical Pharmacologist’s Perspective

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Unveiling the new chapter in nanobody engineering: advances in traditional construction and AI-driven optimization

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A comparison of the binding sites of antibodies and single-domain antibodies

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Current status and future expectations of nanobodies in oncology trials

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Nanobody engineering: computational modelling and design for biomedical and therapeutic applications

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Single-Domain Antibodies Efficiently Neutralize SARS-CoV-2 Variants of Concern

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Molecular basis for thermal stability and affinity in a VHH: Contribution of the framework region and its influence in the conformation of the CDR3

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Identification of conformation-selective nanobodies against the membrane protein insertase BamA by an integrated structural biology approach

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Advancements in Nanobody Epitope Prediction: A Comparative Study of AlphaFold2Multimer vs AlphaFold3

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Single-domain antibodies as therapeutics for solid tumor treatment

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Delicate balance among thermal stability, binding affinity, and conformational space explored by single-domain VHH antibodies

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Nanobodies targeting immune checkpoint molecules for tumor immunotherapy and immunoimaging (Review)

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Downsizing antibodies: Towards complementarity-determining region (CDR)-based peptide mimetics

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Small but mighty: the versatility of nanobodies in modern medicine

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Nanobodies – Little helpers unravelling intracellular signaling

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Structural Classification of CDR-H3 in Single-Domain VHH Antibodies

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Research progress on unique paratope structure, antigen binding modes, and systematic mutagenesis strategies of single-domain antibodies

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Expanding and improving nanobody repertoires using a yeast display method: Targeting SARS-CoV-2

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From discovery to the clinic: structural insights, engineering options, clinical, and ‘next wave’ applications of camelid-derived single-domain antibodies

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Identification, binding, and structural characterization of single domain anti-PD-L1 antibodies inhibitory of immune regulatory proteins PD-1 and CD80

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Identification of nanobodies against hepatocellular carcinoma marker glypican-3

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Structure and Dynamics Guiding Design of Antibody Therapeutics and Vaccines

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Unique mechanisms to increase structural stability and enhance antigen binding in nanobodies

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Arabidopsis immunity regulator EDS1 in a PAD4/SAG101-unbound form is a monomer with an inherently inactive conformation

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A nanobody toolbox targeting dimeric coiled-coil modules for functionalization of designed protein origami structures

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NbX: Machine Learning-Guided Re-Ranking of Nanobody–Antigen Binding Poses

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A non-redundant data set of nanobody-antigen crystal structures

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In Vitro Affinity Maturation of Nanobodies against Mpox Virus A29 Protein Based on Computer-Aided Design

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Nanobody Paratope Ensembles in Solution Characterized by MD Simulations and NMR

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Tuning Nanobodies’ Bioactivity: Coupling to Ultrasmall Gold Nanoparticles Allows the Intracellular Interference with Survivin

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Single‐Domain Antibody‐Based TCR‐Like CAR‐T: A Potential Cancer Therapy

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Nanobody-Based Drug Delivery: Emerging Strategies for Targeted Cancer Therapy

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PLAbDab-nano: a database of camelid and shark nanobodies from patents and literature

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Isolation and structural characterization of a Zn2+-bound single-domain antibody against NorC, a putative multidrug efflux transporter in bacteria

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Computer-guided library generation applied to the optimization of single-domain antibodies

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A whole-cell platform for discovering synthetic cell adhesion molecules in bacteria

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Development of novel humanized VHH synthetic libraries based on physicochemical analyses

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Interaction standards for biophysics: anti-lysozyme nanobodies

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Development of nanobodies against Staphylococcus enterotoxin B through yeast surface display

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Broad Reactivity Single Domain Antibodies against Influenza Virus and Their Applications to Vaccine Potency Testing and Immunotherapy

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Structural Plausibility Without Binding Specificity: Limits of AI-Based Antibody-Antigen Structure Prediction Confidence ScoresPreprint

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Bypassing the Need for Cell Permeabilization: Nanobody CDR3 Peptide Improves Binding on Living Bacteria

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Development of a double antibody sandwich ELISA method for the quantitative detection of serum C-reactive protein based on nanobody

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Characterising nanobody developability to improve therapeutic design using the Therapeutic Nanobody Profiler

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Targeting Ras-binding domain of ELMO1 by computational nanobody design

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Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies

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A single-domain antibody library based on a stability-engineered human VH3 scaffold

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The role of electrochemical biosensors in monitoring Apolipoprotein B-100 as a diagnostic tool for cardiovascular disease

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Confidence scoring for deep learning-predicted antibody–antigen complexes: AntiConf as a precision-driven metric

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An artificial neural network model to predict structure-based protein–protein free energy of binding from Rosetta-calculated properties

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A selection and optimization strategy for single-domain antibodies targeting the PHF6 linear peptide within the tau intrinsically disordered protein

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Paratope Duality and Gullying are Among the Atypical Recognition Mechanisms Used by a Trio of Nanobodies to Differentiate Ebolavirus Nucleoproteins

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Production of Single-Domain Antibodies in Pichia pastoris

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Branched Polyethylenimine as a Carrier for Significantly Improving the Biopanning Efficiency of Phages Specific to Hapten

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Structural insights into the role and targeting of EGFRvIII

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Nanobodies: A Promising Toolkit for Diagnostic Applications

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Conformational features and interaction mechanisms of VHH antibodies with β‐hairpin CDR3 : A case of Nb8‐HigB2 interaction

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In vitro inhibition of cancer angiogenesis and migration by a nanobody that targets the orphan receptor Tie1

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Designing stable humanized antibodies

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Nanobody CAR-T cells in cancer: From molecular design to clinical translation

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Applications of High-Throughput DNA Sequencing to Single-Domain Antibody Discovery and Engineering

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Single-chain dimers from de novo immunoglobulins as robust scaffolds for multiple binding loops

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Development of a new affinity maturation protocol for the construction of an internalizing anti-nucleolin antibody library

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Enthalpy‐ versus Entropy‐Driven Molecular Recognition in the Era of Biologics

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Development of a synthetic library of humanized nanobodies for targeted IL-6 inhibition

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Nanobody-as versatile tool emerging in autoimmune diseases

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Affinity‐stability trade‐off mechanism of residue 35 in framework region 2 of VHH antibodies with β‐hairpin CDR3

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Site-Specific Fluorescent Labeling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells

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Role of the non‐hypervariable FR3 D‐E loop in single‐domain antibody recognition of haptens and carbohydrates

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Potential and limitations of epitope mapping and molecular targeting in Hymenoptera venom allergy

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Nanobody-based imaging: Advancing precision in molecular diagnostics

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Analysis of nanobody paratopes reveals greater diversity than classical antibodies

Mitchell, L. S., & Colwell, L. J. (2018) · Protein Engineering Design and Selection · doi:10.1093/protein/gzy017 · Cited by 138 · see citing works

Integrative proteomics identifies thousands of distinct, multi-epitope, and high-affinity nanobodies

Xiang, Y., Sang, Z., Bitton, L., Xu, J., Liu, Y., Schneidman‐Duhovny, D., & Shi, Y. (2021) · Cell Systems · doi:10.1016/j.cels.2021.01.003 · Cited by 61 · see citing works

Using data to characterise the relationship between nanobody sequence, structure and function

Mitchell, L. (2018) · Apollo (University of Cambridge) · doi:10.17863/cam.42267

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Structural Characterization of Nanobodies during Germline Maturation

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