The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Ski regulates proliferation and migration of reactive astrocytes induced by lipopolysaccharide (LPS) through PI3K/Akt pathway
Abstract
Spinal cord injury (SCI) is a leading cause of disability and death worldwide. Reactive astrogliosis, a typical feature of SCI, undergoes various molecular and morphological changes and contributes to glial scar formation, which impedes axonal regeneration. Ski is a novel molecule that regulates the biological characteristics of astrocytes after spinal cord injury, but its function and the exact mechanism of its overexpression in reactive astrocyte proliferation and migration after SCI remain unclear. The purpose of this study was to elucidate the effect and mechanism of Ski on the proliferation and migration of reactive astrocytes, and to regulate the spatiotemporal formation of glial scars after SCI. In an in vitro lipopolysaccharide (LPS)-induced astrocyte injury model, the expression of Ski was upregulated in a time-dependent manner in LPS-induced astrocytes, and the upregulation of Ski was accompanied by that of PCNA, CDK4, CyclinD1, and other proliferation-related proteins. Our findings suggest that Ski promotes the proliferation and migration of reactive astrocytes. Next, astrocytes were transfected with a specific lentivirus to cause the overexpression of Ski, which significantly enhanced the proliferation and migration of reactive astrocytes and LPS-induced activation of the PI3K/Akt pathway. The PI3K/Akt pathway inhibitor LY294002 significantly inhibited the proliferation and migration of LPS-induced reactive astrocytes after Ski overexpression. In conclusion, Ski r…
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
PI3K-Akt signaling can regulate actin activity and is important in astrocyte proliferation and migration.
“It has been suggested that PI3K-Akt signaling can regulate actin activity and is important in astrocyte proliferation and migration (Zhao et al., 2017;Liao et al., 2022;Pérez-Núñez et al., 2023).”
From Cell Adhesion Molecule Protocadherin-γC5 Ameliorates Aβ Plaque Pathogenesis by Modulating Astrocyte Function in Alzheimer's Disease (2025) · cites this paper as Liao et al., 2022
Citations
Cites
Astrocytes: Biology and pathology
Sofroniew, M. V., & Vinters, H. V. (2010) · Acta Neuropathologica · doi:10.1007/s00401-009-0619-8 · Cited by 5,196
Regeneration beyond the glial scar
Silver, J., & Miller, J. H. (2004) · Nature reviews. Neuroscience · doi:10.1038/nrn1326 · Cited by 3,151
Molecular dissection of reactive astrogliosis and glial scar formation
Sofroniew, M. V. (2009) · Trends in Neurosciences · doi:10.1016/j.tins.2009.08.002 · Cited by 2,594
The glial scar and central nervous system repair
Fawcett, J. W., & Asher, R. A. (1999) · Brain Research Bulletin · doi:10.1016/s0361-9230(99)00072-6 · Cited by 1,808
Plasticity of cell migration: a multiscale tuning model
Friedl, P., & Wolf, K. (2009) · The Journal of Cell Biology · doi:10.1083/jcb.200909003 · Cited by 1,356
Middeldorp, J., & Hol, E. M. (2011) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2011.01.005 · Cited by 1,080
Therapeutic interventions after spinal cord injury
Thuret, S., Moon, L. D. F., & Gage, F. H. (2006) · Nature reviews. Neuroscience · doi:10.1038/nrn1955 · Cited by 1,022
Fitch, M. T., & Silver, J. (2008) · Experimental Neurology · doi:10.1016/j.expneurol.2007.05.014 · Cited by 1,007
Hol, E. M., & Pekny, M. (2015) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2015.02.004 · Cited by 917
Astrocyte dysfunction in neurological disorders: a molecular perspective
Seifert, G., Schilling, K., & Steinhäuser, C. (2006) · Nature reviews. Neuroscience · doi:10.1038/nrn1870 · Cited by 811
Moving beyond the glial scar for spinal cord repair
Bradbury, E. J., & Burnside, E. R. (2019) · Nature Communications · doi:10.1038/s41467-019-11707-7 · Cited by 761
Acute Spinal Cord Injury, Part I: Pathophysiologic Mechanisms
Dumont, R. J., Okonkwo, D. O., Verma, S., Hurlbert, R. J., Boulos, P. T., Ellegala, D. B., & Dumont, A. S. (2001) · Clinical Neuropharmacology · doi:10.1097/00002826-200109000-00002 · Cited by 733
Pathophysiology and pharmacologic treatment of acute spinal cord injury*1
Kwon, B. K. (2004) · The Spine Journal · doi:10.1016/j.spinee.2003.07.007 · Cited by 705
Sofroniew, M. V. (2015) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a020420 · Cited by 695
Functional regeneration beyond the glial scar
Cregg, J. M., DePaul, M. A., Filous, A. R., Lang, B. T., Tran, A. P., & Silver, J. (2014) · Experimental Neurology · doi:10.1016/j.expneurol.2013.12.024 · Cited by 686
The role of extracellular matrix in CNS regeneration
Busch, S. A., & Silver, J. (2007) · Current Opinion in Neurobiology · doi:10.1016/j.conb.2006.09.004 · Cited by 468
The diversity and disparity of the glial scar
Adams, K. L., & Gallo, V. (2017) · Nature Neuroscience · doi:10.1038/s41593-017-0033-9 · Cited by 445
Astrocytes Block Axonal Regeneration in Mammals by Activating the Physiological Stop Pathway
Liuzzi, F. J., & Lasek, R. J. (1987) · Science · doi:10.1126/science.3603044 · Cited by 397
Inhibition of neurite outgrowth on astroglial scars in vitro
Rudge, J., & Silver, J. (1990) · Journal of Neuroscience · doi:10.1523/jneurosci.10-11-03594.1990 · Cited by 381
Astrocyte reactivity and astrogliosis after spinal cord injury
Okada, S., Hara, M., Kobayakawa, K., Matsumoto, Y., & Nakashima, Y. (2018) · Neuroscience Research · doi:10.1016/j.neures.2017.10.004 · Cited by 313
Glial cell extracellular matrix: boundaries for axon growth in development and regeneration
Fitch, M. T., & Silver, J. (1997) · Cell and Tissue Research · doi:10.1007/s004410050944 · Cited by 229
Spinal Cord Injury and Related Clinical Trials
Kim, Y.-H., Ha, K.-Y., & Kim, S.-I. (2017) · Clinics in Orthopedic Surgery · doi:10.4055/cios.2017.9.1.1 · Cited by 155
Concise Review: Reactive Astrocytes and Stem Cells in Spinal Cord Injury: Good Guys or Bad Guys?
Lukovic, D., Stojković, M., Moreno‐Manzano, V., Jendelová, P., Syková, E., Bhattacharya, S. S., & Erceg, S. (2015) · Stem Cells · doi:10.1002/stem.1959 · Cited by 123
Suzuki, H., Yagi, K., Kondo, M., Kato, M., Miyazono, K., & Miyazawa, K. (2004) · Oncogene · doi:10.1038/sj.onc.1207690 · Cited by 116
Macías‐Silva, M., Li, W., Leu, J. I.-J., Crissey, M. A. S., & Taub, R. A. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202403200 · Cited by 99
Sticozzi, C., Belmonte, G., Meini, A., Carbotti, P., Grasso, G., & Palmi, M. (2013) · Neuroscience · doi:10.1016/j.neuroscience.2013.07.061 · Cited by 85
Amyloid β‐induced astrogliosis is mediated by β1‐integrin via NADPH oxidase 2 in Alzheimer's disease
Wyssenbach, A., Quintela‐López, T., Llavero, F., Zugaza, J. L., Matute, C., & Alberdi, E. M. (2016) · Aging Cell · doi:10.1111/acel.12521 · Cited by 81
PI3K and MAPK pathways mediate the BDNF/TrkB-increased metastasis in neuroblastoma
Hua, Z., Gu, X., Dong, Y., Tan, F., Liu, Z., Thiele, C. J., & Li, Z. (2016) · Tumor Biology · doi:10.1007/s13277-016-5433-z · Cited by 75
Yang, J.-W., Ru, J., Ma, W., Gao, Y., Liang, Z., Liu, J., Guo, J.-H., & Li, L.-Y. (2015) · Neuropeptides · doi:10.1016/j.npep.2015.08.005 · Cited by 70
The Protooncogene Ski Controls Schwann Cell Proliferation and Myelination
Atanasoski, S., Notterpek, L. M., Lee, H.-Y., Castagner, F., Young, P., Ehrengruber, M. U., Meijer, D., Sommer, L., Stavnezer, E., Colmenares, C., et al. (2004) · Neuron · doi:10.1016/j.neuron.2004.08.001 · Cited by 70
Advances in the Management of Spinal Cord Injury
Gupta, R., Bathen, M., Smith, J., Levi, A. D., Bhatia, N. N., & Steward, O. (2010) · Journal of the American Academy of Orthopaedic Surgeons · doi:10.5435/00124635-201004000-00004 · Cited by 69
Wu, D., Klaw, M. C., Connors, T. M., Kholodilov, N. G., Burke, R. E., & Tom, V. J. (2015) · Journal of Neuroscience · doi:10.1523/jneurosci.0719-15.2015 · Cited by 62
Ski, a modulator of wound healing and scar formation in the rat skin and rabbit ear
Li, P., Liu, P., Xiong, R., Chen, X., Zhao, Y., Lu, W., Liu, X., Ning, Y., Yang, N., & Zhou, Y. (2010) · The Journal of Pathology · doi:10.1002/path.2831 · Cited by 55
Zhang, K., Wu, S., Li, Z., & Zhou, J. (2017) · Bioscience Reports · doi:10.1042/bsr20170755 · Cited by 52
Bonnon, C., & Atanasoski, S. (2011) · Cell and Tissue Research · doi:10.1007/s00441-011-1180-z · Cited by 51
Zhang, H., & Stavnezer, E. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m807526200 · Cited by 45
Self-citationIncreased responses in the somatosensory thalamus immediately after spinal cord injury
Alonso‐Calviño, E., Martínez-Camero, I., Fernández‐López, E., Humanes-Valera, D., Foffani, G., & Aguilar, J. (2015) · Neurobiology of Disease · doi:10.1016/j.nbd.2015.12.003 · Cited by 39
Xu, L., Tang, Y.-Y., Ben, X.-L., Cheng, M.-H., Guo, W., Liu, Y., Lu, Z., & Deng, J. (2020) · Life Sciences · doi:10.1016/j.lfs.2020.117642 · Cited by 31
Li, J., Li, P., Zhang, Y., Li, G., Zhou, Y., Yang, K., & Dai, S.-S. (2012) · Cellular Signalling · doi:10.1016/j.cellsig.2012.09.001 · Cited by 26
Anti-inflammatory properties of the glial scar
Coulson‐Thomas, V. J., Gesteira, T. F., & Coulson‐Thomas, Y. M. (2016) · Neural Regeneration Research · doi:10.4103/1673-5374.194710 · Cited by 24
Expression of Ski and its role in astrocyte proliferation and migration
Zhao, X., Wang, X.-W., Zhou, K.-S., Nan, W., Guo, Y.-Q., Kou, J.-L., Wang, J., Xia, Y.-Y., & Zhang, H.-H. (2017) · Neuroscience · doi:10.1016/j.neuroscience.2017.08.027 · Cited by 22
Self-citationSpatiotemporal expression of Ski after rat spinal cord injury
Zhou, K., Nan, W., Feng, D., Yi, Z., Zhu, Y., Long, Z., Li, S., Zhang, H., & Wu, Y. (2017) · Neuroreport · doi:10.1097/wnr.0000000000000729 · Cited by 21
Self-citationEffects of Mitofusin2 on astrocytes proliferation in vitro induced by scratch injury
Shi, Y., Luo, P., Yi, C., Xie, J., & Zhang, Q. (2020) · Neuroscience Letters · doi:10.1016/j.neulet.2020.134969 · Cited by 4
Flagged first, then by citations
Cited by
Sorted by citations
The role of PI3K/Akt signalling pathway in spinal cord injury
Xiao, C.-L., Yin, W.-C., Zhong, Y., Luo, J.-Q., Liu, L., Liu, W.-Y., & Zhao, K. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.113881 · Cited by 95
Vera, D. M., Zambrana-Infantes, E. N., López-Gambero, A. J., Verheul-Campos, J., Santín, L. J., Baixerás, E., Suárez, J., Pavón, F. J., Rosell‐Valle, C., & de Fonseca, F. R. (2023) · Neurobiology of Disease · doi:10.1016/j.nbd.2023.106295 · Cited by 17
Pérez-Núñez, R., González, M. F., Avalos, A. M., & Leyton, L. (2024) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-23-01756 · Cited by 17
Hemati-Gourabi, M., Cao, T., Mills, A. E., Rice, E. P., Baur, L., Xu, X. Q., Fenske, W. K., & Chen, M. (2025) · Experimental Neurology · doi:10.1016/j.expneurol.2025.115379 · Cited by 5
Peng, Y., Xiong, R., Wang, B., Chen, X., Ning, Y., Zhao, Y., Yang, N., Zhang, J., Li, C., Zhou, Y., et al. (2024) · International Journal of Biological Sciences · doi:10.7150/ijbs.98856 · Cited by 5
Zhang, Y., Yang, Y.-S., Chen, W.-C., Wang, C., & He, H. (2023) · Molecular Neurobiology · doi:10.1007/s12035-023-03510-9 · Cited by 5
Sun, X., Pan, S., Li, D., Su, M., Zheng, H., Zhang, Y.-W., & Li, Y. (2025) · The Journal of Neuroscience · doi:10.1523/jneurosci.0967-24.2025 · Cited by 4
Yu, L., Qi, S., Wei, G., Rao, X., Luo, D., Zou, M., Mi, Y., Zhang, C., & Li, J. (2023) · Cell Cycle · doi:10.1080/15384101.2023.2278938 · Cited by 4
Lentivirus-mediated Knockdown of Ski Improves Neurological Function After Spinal Cord Injury in Rats
Wang, Z., Ran, R., Ma, C.-W., Zhao, G., Zhou, K., & Zhang, H. (2024) · Neurochemical Research · doi:10.1007/s11064-024-04261-2 · Cited by 2
Self-citation陈太邦, Yu, L., Cai, Z., & Chen, L. J. (2026) · Molecular Neurobiology · doi:10.1007/s12035-026-05792-1 · Cited by 1
Grison, A., Karimaddini, Z., Breda, J., Mukhtar, T., Boareto, M., Eschbach, K., Beisel, C., Iber, D., van Nimwegen, E., Taylor, V., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.12.16.520470
Wang, J., Liang, J., Liang, Y., Li, Y., Huang, Z., Guo, W., Zhou, X., & Zhu, L. (2025) · International Immunopharmacology · doi:10.1016/j.intimp.2025.115835
Pedra, N. S., de Aguiar, M. S. S., Teixeira, F. C., de Mello, J. E., Bona, N. P., da Silveira, L. M., Rosa, T. M. D., Saraiva, J. T., Simões, W. S., Leal, D. B. R., et al. (2026) · ACS Omega · doi:10.1021/acsomega.6c02414
Metadata
- Authors
- Liao, H.-Y., Wang, Z.-Q., Da, C.-M., Zhou, K.-S., & Zhang, H.-H.
- Affiliations
- Lanzhou University Second Hospital (CN)
- Corresponding author
- Zhou, Kai-Sheng
- ORCID
- Liao, Hai-Yang
- Venue
- Journal of Neuroimmunology
- Publisher
- Elsevier BV
- Published
- 2022-03
- Volume
- 364
- Pages
- 577807
- ISSN
- 0165-5728 (ISSN-L)
- OpenAlex
- W4205741329
- Cited by
- 13
- References
- 43
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 2
- FWCI
- 1.83
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Neuroimmunology (publishedVersion)
- PubMed (publishedVersion)
- Lanzhou University Institutional Repository (submittedVersion)
- Topics
- Health Sciences › Medicine › Clinical Neurology › Spinal Cord Injury Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Clinical Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Keywords
- skiing, reactive astrogliosis, spinal cord injury, astrocyte proliferation, astrocyte migration, glial scar formation, PI3K/Akt signaling, lipopolysaccharide, CDK4, cyclin D1, PCNA, LY294002
- MeSH terms
- Animals, Astrocytes, Cell Movement, Gliosis, Lipopolysaccharides, Proto-Oncogene Proteins, Spinal Cord Injuries, Signal Transduction, Rats, Sprague-Dawley, Phosphatidylinositol 3-Kinases, Cell Proliferation, Proto-Oncogene Proteins c-akt, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
