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Cited by 147Photodynamic Therapy Research StudiesLaser Applications in Dentistry and MedicineAntifungal resistance and susceptibility

Photosensitization of different Candida species by low power laser light

de Souza, Sandra Cristina, Junqueira, Juliana Campos, Balducci, Ivan, Koga-Ito, Cristiane Yumi, Munin, Egberto, Jorge, Antonio Olavo Cardoso·2006·Journal of Photochemistry and Photobiology B Biology
DOI 10.1016/j.jphotobiol.2005.12.002
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Abstract

The aim of this study was to evaluate the effects of the laser radiation (685 nm) associated with photosensitizers on viability of different species of Candida genus. Suspensions of Candida albicans, Candida dubliniensis, Candida krusei and Candida tropicalis, containing 10(6) viable cells per milliliter were obtained with the aid of a Neubauer's chamber. From each species, 10 samples of the cell suspension were irradiated with diode laser (685 nm) with 28 J/cm2 in the presence of methylene blue (0.1 mg/ml), 10 samples were only treated with methylene blue, 10 samples were irradiated with laser in the absence of the dye, 10 samples were treated with the dye and irradiated with laser light and 10 samples were exposed to neither the laser light nor to the methylene blue dye. From each sample, serial dilutions of 10(-2) and 10(-3) were obtained and aliquots of 0.1 ml of each dilution were plated in duplicate on Sabouraud dextrose agar. After incubation at 37 degrees C for 48 h, the number of colony-forming units (CFU/ml) was obtained and data were submitted to ANOVA and Tukey's test (p<0.05). Laser radiation in the presence of methylene blue reduced the number of CFU/ml in 88.6% for C. albicans, 84.8% for C. dubliniensis, 91.6% for C. krusei and 82.3% for C. tropicalis. Despite this, only laser radiation or methylene blue did not reduce significantly the number of CFU/ml of Candida samples, except for C. tropicalis. It could be concluded that the photo activation of methylene blue by the red laser radiation at 685 nm presented fungicide effect on all Candida species studied.

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  • Many studies have demonstrated the efficacy of phenothiazine dyes such as methylene blue, new methylene blue, and toluidine blue in PDT for the reduction of fungi.

    “Many studies have demonstrated the efficacy of phenothiazine dyes such as methylene blue, new methylene blue, and toluidine blue in PDT for the reduction of fungi [256,258,259,261].”

    From Candida glabrata Biofilms: How Far Have We Come? (2017) · cites this paper as [258]

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Authors
de Souza, S. C., Junqueira, J. C., Balducci, I., Koga-Ito, C. Y., Munin, E., & Jorge, A. O. C.
Affiliations
  • Universidade Estadual Paulista (Unesp) (BR)
  • Universidade do Vale do Paraíba (BR)
Corresponding author
Munin, Egberto
ORCID
Venue
Journal of Photochemistry and Photobiology B Biology
Publisher
Elsevier BV
Published
2006-04
Volume
83
Issue
1
Pages
34-38
ISSN
1011-1344 (ISSN-L)
DOI
10.1016/j.jphotobiol.2005.12.002
OpenAlex
W2144702393
Cited by
147
References
32
Citations by year
2026: 1 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 2 · 2021: 10 · 2020: 5 · 2019: 3 · 2018: 11 · 2017: 6 · 2016: 12 · 2015: 10 · 2014: 11 · 2013: 11 · 2012: 8
FWCI
7.33
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Photochemistry and Photobiology B Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Americanae (AECID Library) (publishedVersion)
  • Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion)
  • UNESP Institutional Repository (São Paulo State University) (submittedVersion)
  • LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (submittedVersion)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Laser Applications in Dentistry and Medicine
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Keywords
Candida, photosensitization, methylene blue, red laser diodes, Candida albicans, Candida dubliniensis, Candida krusei, Candida tropicalis, antifungal activity, fungal viability, photodynamic therapy
MeSH terms
Candida, Candida albicans, Lasers, Methylene Blue, Microbial Sensitivity Tests, Photosensitizing Agents, Candida tropicalis

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