The DOI matches its Crossref record.
Feature point tracking and trajectory analysis for video imaging in cell biology
Open accessAbstract
This paper presents a computationally efficient, two-dimensional, feature point tracking algorithm for the automated detection and quantitative analysis of particle trajectories as recorded by video imaging in cell biology. The tracking process requires no a priori mathematical modeling of the motion, it is self-initializing, it discriminates spurious detections, and it can handle temporary occlusion as well as particle appearance and disappearance from the image region. The efficiency of the algorithm is validated on synthetic video data where it is compared to existing methods and its accuracy and precision are assessed for a wide range of signal-to-noise ratios. The algorithm is well suited for video imaging in cell biology relying on low-intensity fluorescence microscopy. Its applicability is demonstrated in three case studies involving transport of low-density lipoproteins in endosomes, motion of fluorescently labeled Adenovirus-2 particles along microtubules, and tracking of quantum dots on the plasma membrane of live cells. The present automated tracking process enables the quantification of dispersive processes in cell biology using techniques such as moment scaling spectra.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Inferring the structure and dynamics of interactions in schooling fish
Katz, Y., Tunstrøm, K., Ioannou, C. C., Huepe, C., & Couzin, I. D. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1107583108 · Cited by 927
Manderson, A. P., Kay, J. G., Hammond, L. A., Brown, D. L., & Stow, J. L. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200612131 · Cited by 198
Jacquemet, G., Baghirov, H., Georgiadou, M., Sihto, H., Peuhu, E., Cettour-Janet, P., He, T., Perälä, M., Kronqvist, P., Joensuu, H., et al. (2016) · Nature Communications · doi:10.1038/ncomms13297 · Cited by 186
Ciliogenesis is regulated by a huntingtin-HAP1-PCM1 pathway and is altered in Huntington disease
Keryer, G., Pineda, J. R., Liot, G., Kim, J., Dietrich, P., Benstaali, C., Smith, K., Cordelières, F. P., Spassky, N., Ferrante, R. J., et al. (2011) · Journal of Clinical Investigation · doi:10.1172/jci57552 · Cited by 168
Tau protein diffuses along the microtubule lattice
Hinrichs, M. H., Jalal, A., Brenner, B., Mandelkow, E., Kumar, S., & Scholz, T. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.369785 · Cited by 141
Contact-induced clustering of syntaxin and munc18 docks secretory granules at the exocytosis site
Gandasi, N. R., & Barg, S. (2014) · Nature Communications · doi:10.1038/ncomms4914 · Cited by 130
Mobilization of HIV Spread by Diaphanous 2 Dependent Filopodia in Infected Dendritic Cells
Aggarwal, A., Iemma, T. L., Shih, I., Newsome, T. P., McAllery, S., Cunningham, A. L., & Turville, S. G. (2012) · PLoS Pathogens · doi:10.1371/journal.ppat.1002762 · Cited by 115
Histone deacetylase 8 is required for centrosome cohesion and influenza A virus entry
Yamauchi, Y., Boukari, H., Banerjee, I., Sbalzarini, I. F., Horvath, P., & Helenius, A. (2011) · PLoS Pathogens · doi:10.1371/journal.ppat.1002316 · Cited by 84
Self-citationPtdIns(3,4,5)P 3 is a regulator of myosin-X localization and filopodia formation
Plantard, L., Arjonen, A., Lock, J. G., Nurani, G., Ivaska, J., & Strömblad, S. (2010) · Journal of Cell Science · doi:10.1242/jcs.069609 · Cited by 84
Scotti, E., Calamai, M., Goulbourne, C. N., Zhang, L., Hong, C., Lin, R. R., Choi, J., Pilch, P. F., Fong, L. G., Zou, P., et al. (2013) · Molecular and Cellular Biology · doi:10.1128/mcb.01716-12 · Cited by 81
Walentek, P., Bogusch, S., Thumberger, T., Vick, P., Dubaissi, E., Beyer, T., Blum, M., & Schweickert, A. (2014) · Development · doi:10.1242/dev.102343 · Cited by 61
High-Speed Chemical Imaging by Dense-Net Learning of Femtosecond Stimulated Raman Scattering
Zhang, J., Zhao, J., Lin, H., Tan, Y., & Cheng, J.-X. (2020) · The Journal of Physical Chemistry Letters · doi:10.1021/acs.jpclett.0c01598 · Cited by 54
The conserved ciliary protein Bug22 controls planar beating of Chlamydomonas flagella
Meng, D., Cao, M., Oda, T., Kikkawa, M., & Pan, J. (2013) · Journal of Cell Science · doi:10.1242/jcs.140723 · Cited by 33
The Emergence of Functional Ultrasound for Noninvasive Brain–Computer Interface
Zheng, H., Niu, L., Qiu, W., Liang, D., Long, X., Li, G., Liu, Z., & Meng, L. (2023) · Research · doi:10.34133/research.0200 · Cited by 27
Annibale, P., & Gratton, E. (2015) · Scientific Reports · doi:10.1038/srep09258 · Cited by 24
Autoregulation of the real-time kinetics of the human mitochondrial replicative helicase
Plaza-G A, I., Ortiz-Rodríguez, M., Buchanan, S. P., Miguez-Amil, S., Lemishko, K. M., Moreno-Herrero, F., Fernandez-Leiro, R., Ciesielski, G. L., & Ibarra, B. (2025) · Nature Communications · doi:10.1038/s41467-025-60289-0 · Cited by 6
TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing factors
Bhuiyan, T., Arecco, N., Mendoza Sanchez, P. K., Kim, J., Schwan, C., Weyrauch, S., Nizamuddin, S., Prunotto, A., Tekman, M., Biniossek, M. L., et al. (2025) · Cell Reports · doi:10.1016/j.celrep.2025.115616 · Cited by 5
DNA polymerase actively and sequentially displaces single-stranded DNA-binding proteins
Xu, L., Jin, S., Urem, M., Lee, S.-J., Lamers, M. H., Chen, X., Wolynes, P. G., & Wuite, G. J. L. (2025) · Nature Communications · doi:10.1038/s41467-025-62531-1 · Cited by 3
Metadata
- Authors
- Sbalzarini, I. F., & Koumoutsakos, P.
- Venue
- Journal of Structural Biology
- Publisher
- Elsevier BV
- Published
- 2005-08
- Volume
- 151
- Issue
- 2
- Pages
- 182-195
- ISSN
- 1047-8477 (ISSN-L)
- OpenAlex
- W2129140439
- Cited by
- 1,392
- References
- 0
- FWCI
- 10.70
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
