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Cited by 5Genomics and Chromatin DynamicsRNA Research and SplicingNuclear Structure and Function

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TAF2 condensation in nuclear speckles links basal transcription factor TFIID to RNA splicing factors

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Bhuiyan, Tanja, Arecco, Niccolò, Mendoza Sanchez, Paulina Karen, Kim, Juhyeong, Schwan, Carsten, Weyrauch, Sophie, Nizamuddin, Sheikh, Prunotto, Andrea, Tekman, Mehmet, Biniossek, Martin L., Knapp, Bettina, Koidl, Stefanie, Drepper, Friedel, Huesgen, Pitter F., Grosse, Robert, Hugel, Thorsten, Arnold, Sebastian J.·2025·Cell Reports
DOI 10.1016/j.celrep.2025.115616

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Abstract

TFIID is an essential basal transcription factor, crucial for RNA polymerase II (pol II) promoter recognition and transcription initiation. The TFIID complex consists of the TATA binding protein (TBP) and 13 TBP-associated factors (TAFs) that contain intrinsically disordered regions (IDRs) with currently unknown functions. Here, we show that a conserved IDR drives TAF2 to nuclear speckle condensates independently of other TFIID subunits. Quantitative mass spectrometry analyses reveal TAF2 proximity to RNA splicing factors including specific interactions of the TAF2 IDR with SRRM2 in nuclear speckles. Deleting the IDR from TAF2 does not majorly impact global gene expression but results in changes of alternative splicing events. Further, genome-wide binding analyses suggest that the TAF2 IDR impedes TAF2 promoter association by guiding TAF2 to nuclear speckles. This study demonstrates that an IDR within the large multiprotein complex TFIID controls nuclear compartmentalization and thus links distinct molecular processes, namely transcription initiation and RNA splicing.

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Metadata

Authors
Bhuiyan, T., Arecco, N., Mendoza Sanchez, P. K., Kim, J., Schwan, C., Weyrauch, S., Nizamuddin, S., Prunotto, A., Tekman, M., Biniossek, M. L., Knapp, B., Koidl, S., Drepper, F., Huesgen, P. F., Grosse, R., Hugel, T., & Arnold, S. J.
Affiliations
  • University of Freiburg (DE)
  • Centre for Genomic Regulation (ES)
Corresponding author
  • Bhuiyan, Tanja
  • Arnold, Sebastian J.
ORCID
Venue
Cell Reports
Publisher
Elsevier BV
Published
2025-05
Volume
44
Issue
5
Pages
115616
ISSN
2211-1247 (ISSN-L)
DOI
10.1016/j.celrep.2025.115616
OpenAlex
W4409790373
Cited by
5
References
4
Citations by year
2026: 2 · 2025: 3
FWCI
1.51
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Reports (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
  • Repositori digital de la UPF (Universitat Pompeu Fabra) (publishedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
Funders
  • European Molecular Biology Organization (LTF 695-2019)
  • German Research Foundation (EXC-2189, 403222702, CRC 1381, INST 39/1170-1 FUGG, 390939984, CRC 992, 192904750, AR 732/3-1)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
TFIID, intrinsically disordered regions, nuclear speckles, condensate, RNA splicing, alternative splicing, SRRM2, TATA-binding protein, transcription initiation, RNA polymerase II, nuclear compartmentalization, protein-protein interaction
MeSH terms
RNA Splicing Factors, Cell Nucleus, HeLa Cells, Humans, Promoter Regions, Genetic, Protein Binding, RNA Splicing, Transcription Factor TFIID, TATA-Binding Protein Associated Factors

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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