Effects of aging and gender on muscle mass and regulation of Akt-mTOR-p70s6k related signaling in the F344BN rat model
Abstract
Sarcopenia is the loss of muscle mass and strength which occurs with aging. Whether the molecular basis of sarcopenia differs with muscle type and across sex is not well understood. Here we examine how aging affects the regulation of protein kinase B (Akt), the mammalian target of rapamycin (mTOR), AMP activated kinase (AMPK), p70 ribosomal S6 kinase (p70s6k), S6 ribosomal protein (rps6) and calcineurin (CaN) in the slow soleus and fast extensor digitorum longus (EDL) muscles of 6- (adult), 30- (aged), and 36-month (very aged) male and 6- (adult), 26- (aged), and 30-month (very aged) female Fischer 344xBrown Norway (F344BN) rats. In male animals, soleus and EDL muscle to body weight ratios decreased steadily with age while in the females, losses remained unchanged after 26 months. These age-related changes in the degree of muscle atrophy across sex were associated with differences in the regulation of Akt, mTOR, and p70s6k in the slow-twitch soleus and the regulation of AMPK, 4EBP1, p70s6k and rpS6 in the fast-twitch EDL. Irrespective of muscle type, aging in both the genders was associated with increased calcineurin expression. Taken together, these data suggest that indices of protein synthesis and muscle adaptation are regulated differently with aging in different muscle types and sex.
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CR fully prevented the impact of aging on the muscle weight to body weight ratio.
“Although CR did not attenuate the effect of aging on skeletal muscle mass, it fully prevented the impact of aging on the muscle weight to body weight ratio, a widely used index of sarcopenia (Edstrom et al., 2006; Paturi et al., 2010; Ezzat-Zadeh et al., 2017).”
From Effects of Aging and Caloric Restriction on Fiber Type Composition, Mitochondrial Morphology and Dynamics in Rat Oxidative and Glycolytic Muscles (2019) · cites this paper as Paturi et al., 2010
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- Authors
- Paturi, S., Gutta, A. K., Katta, A., Kakarla, S. K., Arvapalli, R. K., Gadde, M. K., Nalabotu, S. K., Rice, K. M., Wu, M., & Blough, E.
- Affiliations
- Marshall University (US)
- Corresponding author
- Blough, Eric
- ORCID
- Rice, Kevin M.
- Venue
- Mechanisms of Ageing and Development
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 131
- Issue
- 3
- Pages
- 202-209
- ISSN
- 0047-6374 (ISSN-L)
- OpenAlex
- W2140314962
- Cited by
- 54
- References
- 50
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 4 · 2017: 3 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 3 · 2012: 5
- FWCI
- 1.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Mechanisms of Ageing and Development (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute on Aging (AG-027103-1, R15AG027103)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Health Sciences › Medicine › Physiology › Nutrition and Health in Aging
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Muscle metabolism and nutrition
- Keywords
- sarcopenia, muscle aging, sex differences, muscle mass, Akt signaling, mTOR signaling, p70S6K, AMPK, calcineurin, 4E-BP1, ribosomal protein S6, muscle protein synthesis
- MeSH terms
- Aging, Animals, Female, Male, Mammals, Muscular Atrophy, Muscular Diseases, Rats, Inbred BN, Rats, Inbred F344, Signal Transduction, Muscle, Skeletal, Calcineurin, Sirolimus, Ribosomal Protein S6 Kinases, 70-kDa, Proto-Oncogene Proteins c-akt, Rats, Sarcopenia
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