Cited by 172Fungal and yeast genetics researchBioinformatics and Genomic NetworksMicrobial Metabolic Engineering and Bioproduction

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High-resolution chemical dissection of a model eukaryote reveals targets, pathways and gene functions

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Hoepfner, Dominic, Helliwell, Stephen B., Sadlish, Heather, Schuierer, Sven, Filipuzzi, Ireos, Brachat, Sophie, Bhullar, Bhupinder, Plikat, Uwe, Abraham, Yann, Altorfer, Marc, Aust, Thomas, Baeriswyl, Lukas, Cerino, Raffaele, Chang, Lena, Estoppey, David, Eichenberger, Juerg, Frederiksen, Mathias, Hartmann, Nicole, Hohendahl, Annika, Knapp, Britta, Krastel, Philipp, Melin, Nicolas, Nigsch, Florian, Oakeley, Edward J., Petitjean, Virginie, Petersen, Frank, Riedl, Ralph, Schmitt, Esther K., Staedtler, Frank, Studer, Christian, Tallarico, John A., Wetzel, Stefan, Fishman, Mark C., Porter, Jeffrey A., Movva, N. Rao·2014·Microbiological Research

DOI 10.1016/j.micres.2013.11.004

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Abstract

Due to evolutionary conservation of biology, experimental knowledge captured from genetic studies in eukaryotic model organisms provides insight into human cellular pathways and ultimately physiology. Yeast chemogenomic profiling is a powerful approach for annotating cellular responses to small molecules. Using an optimized platform, we provide the relative sensitivities of the heterozygous and homozygous deletion collections for nearly 1800 biologically active compounds. The data quality enables unique insights into pathways that are sensitive and resistant to a given perturbation, as demonstrated with both known and novel compounds. We present examples of novel compounds that inhibit the therapeutically relevant fatty acid synthase and desaturase (Fas1p and Ole1p), and demonstrate how the individual profiles facilitate hypothesis-driven experiments to delineate compound mechanism of action. Importantly, the scale and diversity of tested compounds yields a dataset where the number of modulated pathways approaches saturation. This resource can be used to map novel biological connections, and also identify functions for unannotated genes. We validated hypotheses generated by global two-way hierarchical clustering of profiles for (i) novel compounds with a similar mechanism of action acting upon microtubules or vacuolar ATPases, and (ii) an un-annotated ORF, YIL060w, that plays a role in respiration in the mitochondria. Finally, we identify and characterize background mutations in the widely used yeast deletion collection which should improve the interpretation of past and future screens throughout the community. This comprehensive resource of cellular responses enables the expansion of our understanding of eukaryotic pathway biology.

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Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J. D., Bussey, H., et al. (1999) · Science · doi:10.1126/science.285.5429.901 · Cited by 3,953
The Genetic Landscape of a Cell
Costanzo, M., Baryshnikova, A., Bellay, J., Kim, Y., Spear, E. D., Sevier, C. S., Ding, H., Koh, J. L. Y., Toufighi, K., Mostafavi, S., et al. (2010) · Science · doi:10.1126/science.1180823 · Cited by 2,162
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Stebbins, C. E., Russo, A. A., Schneider, C. B., Rosen, N. X., Hartl, F. U., & Pavletich, N. P. (1997) · Cell · doi:10.1016/s0092-8674(00)80203-2 · Cited by 1,370
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Hillenmeyer, M. E., Fung, E., Wildenhain, J., Pierce, S. E., Hoon, S. S., Lee, W., Proctor, M. J., St.Onge, R. P., Tyers, M., Koller, D., et al. (2008) · Science · doi:10.1126/science.1150021 · Cited by 978
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Schuldiner, M., Collins, S. R., Thompson, N. J., Denic, V., Bhamidipati, A., Punna, T., Ihmels, J., Andrews, B., Boone, C., Greenblatt, J. F., et al. (2005) · Cell · doi:10.1016/j.cell.2005.08.031 · Cited by 870
Inhibition of actin polymerization by latrunculin A
Coué, M., Brenner, S. L., Spector, I., & Korn, E. D. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)81513-2 · Cited by 824
Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways
Parsons, A. B., Brost, R. L., Ding, H., Li, Z., Zhang, C., Sheikh, B., Brown, G. W., Kane, P. M., Hughes, T. R., & Boone, C. M. (2003) · Nature Biotechnology · doi:10.1038/nbt919 · Cited by 651
hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures.
Borkovich, K. A., Farrelly, F. W., Finkelstein, D. B., Taulien, J., & Lindquist, S. (1989) · Molecular and Cellular Biology · doi:10.1128/mcb.9.9.3919 · Cited by 641
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Rock, F. L., Mao, W., Yaremchuk, A. D., Tukalo, M. A., Crépin, T., Zhou, H., Zhang, Y.-K., Hernandez, V. S., Akama, T., Baker, S. J., et al. (2007) · Science · doi:10.1126/science.1142189 · Cited by 639
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Frederick, C. A., Williams, L. D., Ughetto, G., MAREL, G. A. V. D., BOOM, J. H. V., Rich, A., & Wang, A. H. ‐. (1990) · Biochemistry · doi:10.1021/bi00462a016 · Cited by 578

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Authors: Hoepfner, D., Helliwell, S. B., Sadlish, H., Schuierer, S., Filipuzzi, I., Brachat, S., Bhullar, B., Plikat, U., Abraham, Y., Altorfer, M., Aust, T., Baeriswyl, L., Cerino, R., Chang, L., Estoppey, D., Eichenberger, J., Frederiksen, M., Hartmann, N., Hohendahl, A., Knapp, B., Krastel, P., Melin, N., Nigsch, F., Oakeley, E. J., Petitjean, V., Petersen, F., Riedl, R., Schmitt, E. K., Staedtler, F., Studer, C., Tallarico, J. A., Wetzel, S., Fishman, M. C., Porter, J. A., & Movva, N. R.
Affiliations: Novartis (Switzerland) (CH)
Corresponding author: Hoepfner, Dominic
ORCID: Hoepfner, Dominic
Helliwell, Stephen B.
Schuierer, Sven
Filipuzzi, Ireos
Brachat, Sophie
Abraham, Yann
Altorfer, Marc
Estoppey, David
Frederiksen, Mathias
Hohendahl, Annika
Krastel, Philipp
Nigsch, Florian
Oakeley, Edward J.
Schmitt, Esther K.
Studer, Christian
Tallarico, John A.
Fishman, Mark C.
Venue: Microbiological Research
Publisher: Elsevier BV
Published: 2014-02
Volume: 169
Issue: 2-3
Pages: 107-120
ISSN: 0944-5013 (ISSN-L)
DOI: 10.1016/j.micres.2013.11.004
OpenAlex: W2013242034
Cited by: 166
References: 50
Citations by year: 2026: 5 · 2025: 11 · 2024: 12 · 2023: 6 · 2022: 13 · 2021: 6 · 2020: 11 · 2019: 23 · 2018: 20 · 2017: 23 · 2016: 12 · 2015: 18 · 2014: 5
FWCI: 5.38
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/open-access/userlicense/1.0/ (vor, 589 d)
Locations: Microbiological Research (publishedVersion)
PubMed (publishedVersion)
Funders: Novartis Institutes for BioMedical Research
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: chemogenomics, fatty acid synthase inhibition, microtubule inhibitors, mitochondrial respiration, background mutations, Saccharomyces cerevisiae
MeSH terms: Antifungal Agents, Molecular Sequence Data, Phylogeny, Saccharomyces cerevisiae, Gene Expression Regulation, Fungal, Drug Resistance, Fungal, Saccharomyces cerevisiae Proteins, Biosynthetic Pathways, High-Throughput Screening Assays

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