Selection of new lactic acid bacteria strains bearing probiotic features from mucosal microbiota of healthy calves: Looking for immunobiotics through in vitro and in vivo approaches for immunoprophylaxis applications
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From the birth, since their mucosal microbiota and immune system are not fully developed, newborn calves are susceptible to several mucosal pathogenic microorganisms. Operating through humoral and non-humoral mechanisms in the host, several lactic acid bacteria strains bearing probiotic features are often employed in livestock as food supplement, improving animal production performance, promoting health and reducing the severity of mucosal infections. Accordingly, we isolated, species-level identified and screened for their probiotic potentials seventy lactic acid bacteria strains from upper airway, vaginal and intestinal mucosa of healthy calves. Based on in vitro approaches, we selected three strains: Lactobacillus fermentum V3B-08 isolated from upper airway mucosa, Weissella hellenica V1V-30 isolated from vaginal mucosa and Lactobacillus farciminis B4F-06 isolated from intestinal mucosa were used to mono-colonize germ-free mice in the same site in which these strains were isolated, aiming to characterize their immunomodulatory features. These strains were able to colonize germ-free mice mucosa and trigger sIgA synthesis at a local level, in addition to stimulating, in different ways, adaptive immune responses at a systemic level.
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According to MATS classification, L. helveticus 34.9 can be considered a hydrophilic strain.
“According to this classification, L. helveticus 34.9 can be considered a hydrophilic strain, which is in accordance with the results obtained for L. helveticus T103 [35], L. helveticus R0052 [36], and L. helveticus ATCC 12046 [37].”
From The Biological Role of the S-Layer Produced by Lactobacillus helveticus 34.9 in Cell Protection and Its Probiotic Properties (2024) · cites this paper as [34]
Section 2.4 defines hydrophilic strains as having MATS values of 44.99% or less, but does not give a value for L. helveticus 34.9. Section 3.1 and the strain results concern other species; searches for L. helveticus 34.9 and the named comparator strains found no measurement or classification for them in our copy. (LLM, conf=0.76)
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Chase, C. C. L. (2018) · Veterinary Clinics of North America Food Animal Practice · doi:10.1016/j.cvfa.2017.10.006 · Cited by 63 · see citing works
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“[34].”
- Authors
- Sandes, S., Alvim, L., Silva, B., Acurcio, L., Santos, C., Campos, M., Santos, C., Nicoli, J., Neumann, E., & Nunes, Á.
- Affiliations
- Universidade Federal de Minas Gerais (BR)
- Corresponding author
- Sandes, Sávio
- Venue
- Microbiological Research
- Publisher
- Elsevier BV
- Published
- 2017-07
- Volume
- 200
- Pages
- 1-13
- ISSN
- 0944-5013 (ISSN-L)
- OpenAlex
- W2598214641
- Cited by
- 48
- References
- 56
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 6 · 2023: 1 · 2022: 12 · 2021: 4 · 2020: 10 · 2019: 3 · 2018: 8
- FWCI
- 6.46
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Microbiological Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Pró-Reitoria de Pesquisa, Universidade Federal de Minas Gerais
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (505802/2008-2)
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais (APQ 02003)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Keywords
- lactic acid bacteria, probiotics, immunobiotics, calves, mucosal microbiota, Lactobacillus fermentum, Weissella hellenica, Lactobacillus farciminis, secretory IgA, immunomodulation, germ-free mice, mucosal colonization, upper airway, vaginal mucosa, intestinal mucosa, mucosal infection, immunoprophylaxis
- MeSH terms
- Animals, Antibiosis, Bacterial Adhesion, Bile Acids and Salts, Cattle, DNA, Bacterial, Female, Gastric Juice, Hydrogen Peroxide, Immunoglobulin A, Secretory, Immunization, Intestinal Mucosa, Lactobacillus, Microbial Sensitivity Tests, Mucous Membrane, Colony Count, Microbial, Cytokines, Gram-Positive Bacterial Infections, Nitric Oxide Synthase, Lactic Acid, Probiotics, Genes, rRNA, Mice, Adaptive Immunity, Immunomodulation, Hydrophobic and Hydrophilic Interactions, Weissella, Microbiota
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