Distinct spatiotemporal expression of SAPAP transcripts in the developing rat brain: a novel dendritically localized mRNA
Abstract
The four members of the family of synapse-associated protein 90/postsynaptic density-95-associated proteins (SAPAP1-4) are adapter proteins of postsynaptic density (PSD). They interact with different synaptic scaffolding proteins, cytoskeletal components, and signalling components, and are therefore considered to assemble functional multiprotein units at synapses. Here, we analyzed the spatiotemporal expression of SAPAP1-SAPAP4 genes in postnatal rat brain by in situ hybridization. All four genes are expressed in many brain areas, leading to overlapping yet distinct mRNA distribution patterns. Moreover, two mRNAs encoding distinct SAPAP3 isoforms exhibit basically identical postnatal expression patterns. In the hippocampus, SAPAP1, SAPAP2, and SAPAP4 transcripts are restricted to cell body zones, whereas SAPAP3 mRNAs are also detected in molecular layers. Thus, SAPAP3 is one of the few PSD components whose local synthesis in dendrites may contribute to an input-specific adaptation of dendritic spine function.
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Variable expression of Sapap3 during postnatal synapse maturation has been reported.
“Clearly, the age mismatch for behavioral and neuronal phenotypes in the Sapap3-KO model needs to be addressed, particularly given that variable expression of Sapap3 during postnatal synapse maturation has been reported [24].”
From Projection-specific deficits in synaptic transmission in adult Sapap3-knockout mice (2020) · cites this paper as [24]
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Local Protein Synthesis at Synapses
Steward, O., Dynes, J. L., & Farris, S. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-418675-0.00006-7 · Cited by 3 · see citing works
SAPAP4 Deletion Causes Synaptic Dysfunction in the nucleus accumbens
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- Authors
- Kindler, S., Rehbein, M., Classen, B., Richter, D., & Böckers, T. M.
- Affiliations
- University Medical Center Hamburg-Eppendorf (DE)
- Universität Ulm (DE)
- Corresponding author
- Kindler, Stefan
- ORCID
- Kindler, Stefan
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 2004-07
- Volume
- 126
- Issue
- 1
- Pages
- 14-21
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W2010505153
- Cited by
- 56
- References
- 57
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 1 · 2012: 5
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- postsynaptic density, dendritic mRNA localization, postnatal brain development, hippocampus, molecular layer, dendritic spines, synaptic scaffolding proteins, rat brain
- MeSH terms
- SAP90-PSD95 Associated Proteins, Animals, Animals, Newborn, Brain, Dendrites, Gene Expression Regulation, Nerve Tissue Proteins, RNA, Messenger, Synapses, Time Factors, Rats, Wistar, In Situ Hybridization, Protein Isoforms, Rats
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