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Cited by 383

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Smad Proteins Bind a Conserved RNA Sequence to Promote MicroRNA Maturation by Drosha

Open access
Davis, Brandi N., Hilyard, Aaron C., Nguyen, Peter H., Lagna, Giorgio, Hata, Akiko·2010·Molecular Cell
DOI 10.1016/j.molcel.2010.07.011
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Abstract

Summary The signal transducers of the Transforming Growth Factor β (TGFβ)/Bone Morphogenetic Protein (BMP), the Smads, promote the expression of a subset of miRNAs by facilitating the cleavage reaction by Drosha. The mechanism that limits Smad-mediated processing to a selective group of miRNAs remained hitherto unexplored. In this study, we expand the number of TGFβ/BMP-regulated miRNAs (T/B-miRs) to 20. Interestingly, a majority of T/B-miRs contain a consensus sequence (R-SBE) within the stem region of the primary transcripts of T/B-miRs (pri-T/B-miRs). Here, we demonstrate that Smads directly bind the R-SBE. Mutation of the R-SBE abrogates TGFβ/BMP-induced recruitment of Smads, Drosha, and DGCR8 to pri-T/B-miRs, and impairs their processing, while introduction of R-SBE to unregulated pri-miRNAs is sufficient to recruit Smads and allow regulation by TGFβ/BMP. Thus, Smads are multifunctional proteins which modulate gene expression transcriptionally through DNA binding, and post-transcriptionally by pri-miRNA binding and regulation of miRNA processing.

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Metadata

Authors
Davis, B. N., Hilyard, A. C., Nguyen, P. H., Lagna, G., & Hata, A.
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2010-08
Volume
39
Issue
3
Pages
373-384
ISSN
1097-2765 (ISSN-L)
DOI
10.1016/j.molcel.2010.07.011
OpenAlex
W2007701028
Cited by
383
References
1
FWCI
12.19
Type
journal article
Language
en
Open access
bronze

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