Cited by 567MicroRNA in disease regulationRNA Research and SplicingPlant Molecular Biology Research

The multifunctional RNA-binding protein hnRNP A1 is required for processing of miR-18a

Guil, Sonia, Cáceres, Javier F·2007·Nature Structural & Molecular Biology

Abstract

hnRNP A1 is an RNA-binding protein involved in various aspects of RNA processing. Use of an in vivo cross-linking and immunoprecipitation protocol to find hnRNP A1 RNA targets resulted in the identification of a microRNA (miRNA) precursor, pre-miR-18a. This microRNA is expressed as part of a cluster of intronic RNAs, including miR-17, miR-18a, miR-19a, miR-20a, miR-19b-1 and miR-92, and potentially acts as an oncogene. Here we show that hnRNP A1 binds specifically to the primary RNA sequence pri-miR-18a before Drosha processing. HeLa cells depleted of hnRNP A1 have reduced in vitro processing activity with pri-miR-18a and also show reduced abundances of endogenous pre-miR-18a. Furthermore, we show that hnRNP A1 is required for miR-18a-mediated repression of a target reporter in vivo. These results underscore a previously uncharacterized role for general RNA-binding proteins as auxiliary factors that facilitate the processing of specific miRNAs.

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Crossref · 10.1038/nsmb1250

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  • RBPs modulate miRNA biogenesis, stability, and localization through alternative processing and/or maturation.

    “or RBPs modulate miRNA biogenesis, stability, and localization through alternative processing and/or maturation (Guil and Cáceres 2007; Michlewski and Cáceres 2010, 2019; Choudhury et al. 2013; Treiber et al. 2017; Nussbacher and Yeo 2018; Yeom et al. 2018).”

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Authors
Guil, S., & Cáceres, J. F.
Affiliations
Western General Hospital (GB)
Corresponding author
Cáceres, Javier F
Venue
Nature Structural & Molecular Biology
Published
2007-06-10
Volume
14
Issue
7
Pages
591-596
ISSN
  • 1545-9985 (ISSN-L)
  • 1545-9993
OpenAlex
W2079248832
Cited by
567
References
42
Citations by year
2026: 7 · 2025: 11 · 2024: 10 · 2023: 12 · 2022: 16 · 2021: 36 · 2020: 27 · 2019: 20 · 2018: 26 · 2017: 35 · 2016: 18 · 2015: 40 · 2014: 40 · 2013: 44 · 2012: 41
FWCI
15.27
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Structural & Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
hnRNP A1, miR-18a, microRNA processing, RNA-binding proteins, Drosha, microRNA precursors, miR-17-92 cluster, RNA target identification, gene repression, oncogenic microRNA
MeSH terms
Heterogeneous Nuclear Ribonucleoprotein A1, Gene Expression Regulation, HeLa Cells, Humans, RNA Precursors, RNA-Binding Proteins, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, MicroRNAs, Ribonuclease III

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