The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
An Evolutionarily Conserved Long Noncoding RNA TUNA Controls Pluripotency and Neural Lineage Commitment
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SUMMARY Here, we generated the first genome-scale shRNA library targeting lincRNAs in the mouse. We performed an unbiased loss-of-function study in mouse embryonic stem cells (mESCs) and identified 20 novel lincRNAs involved in the maintenance of pluripotency. Among these, TUNA (Tcl1 Upstream Neuron-Associated lincRNA), was required for pluripotency and formed a complex with three RNA-binding proteins (RBPs). The TUNA–RBP complex was detected at the promoters of Nanog, Sox2, and Fgf4, and knockdown of TUNA or the individual RBPs inhibited neural differentiation of mESCs. TUNA showed striking evolutionary conservation of both sequence and central nervous system-restricted expression in vertebrates. Accordingly, knockdown of tuna in zebrafish caused impaired locomotor function, and TUNA expression in the brains of Huntington’s patients was significantly associated with disease grade. Our results suggest that the lincRNA TUNA plays a vital role in pluripotency and neural differentiation of ESCs and is associated with neurological function of adult vertebrates.
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Metadata
- Authors
- Lin, N., Chang, K.-Y., Li, Z., Gates, K., Rana, Z. A., Dang, J., Zhang, D., Han, T., Yang, C.-S., Cunningham, T. J., Head, S. R., Duester, G., Dong, P. D. S., & Rana, T. M.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2014-03
- Volume
- 53
- Issue
- 6
- Pages
- 1005-1019
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W2008695914
- Cited by
- 393
- References
- 42
- FWCI
- 11.76
- Type
- journal article
- Language
- en
- Open access
- bronze
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