A novel nano-hydroxyapatite—PMMA hybrid scaffolds adopted by conjugated thermal induced phase separation (TIPS) and wet-chemical approach: analysis of its mechanical and biological properties
Abstract
In this study, we report the preparation of nano-hydroxyapatite (nHAp) incorporated poly(methyl methacrylate) (PMMA) scaffolds by conjugated thermal induced phase separation (TIPS) and wet-chemical approach, which essentially facilitates the enhancement of both mechanical as well as biological properties of the scaffolds. The dissolution of PMMA was accomplished by acetone (Ace scaffold), ethanol-water (E-W scaffold) and isopropanol-water (I-W scaffold) mixtures as solvents. The existence of nHAp in PMMA matrix was investigated systematically. The higher degree of porous architecture was achieved from Ace scaffolds compared to both I-W and E-W scaffolds. On the other hand, the dense porous architecture of the I-W scaffold exhibited superior hardness and compressive strength than that of the Ace and E-W scaffolds. All the fabricated samples demonstrated enhanced in vitro bioactivity with respect to increasing immersion period as a result of flower-like in vitro apatite layer formation. The MTT assay was carried out for 1day and 3day culture using Saos-2 osteoblast-like cells, which showed better cell proliferation with increasing culture period owing to the interconnected pore architecture of scaffolds and the rational hemocompatibility as per the ASTM standard F756-00.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
A nano-HA/PMMA scaffold obtained interconnected porosity.
“Scaffolds with interconnected porosity was obtained [116]”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [116]
Cites
Porosity of 3D biomaterial scaffolds and osteogenesis
Karageorgiou, V., & Kaplan, D. L. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.02.002 · Cited by 6,639 · see citing works
Nanoscale hydroxyapatite particles for bone tissue engineering
Zhou, H., & Lee, J. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.03.019 · Cited by 1,544 · see citing works
Wei, G., & Ma, P. X. (2004) · Biomaterials · doi:10.1016/j.biomaterials.2003.12.005 · Cited by 1,271 · see citing works
45S5 Bioglass®-derived glass–ceramic scaffolds for bone tissue engineering
Chen, Q. Z., Thompson, I. D., & Boccaccini, A. R. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.11.025 · Cited by 1,244 · see citing works
Understanding the effect of mean pore size on cell activity in collagen-glycosaminoglycan scaffolds
Murphy, C. M., & O’Brien, F. J. (2010) · Cell Adhesion & Migration · doi:10.4161/cam.4.3.11747 · Cited by 642 · see citing works
A novel method to fabricate bioabsorbable scaffolds
Whang, K., Thomas, C. H., Healy, K. E., & Nuber, G. (1995) · Polymer · doi:10.1016/0032-3861(95)93115-3 · Cited by 639 · see citing works
Osteoinduction in porous hydroxyapatite implanted in heterotopic sites of different animal models
Ripamonti, U. (1996) · Biomaterials · doi:10.1016/0142-9612(96)80752-6 · Cited by 499 · see citing works
Preparation of porous scaffolds by using freeze-extraction and freeze-gelation methods
Ho, M.-H., Kuo, P.-Y., Hsieh, H.-J., Hsien, T.-Y., Hou, L.-T., Lai, J.-Y., & Wang, D.-M. (2004) · Biomaterials · doi:10.1016/s0142-9612(03)00483-6 · Cited by 484 · see citing works
Polymer concepts in tissue engineering
Peter, S. J., Miller, M. J., Yasko, A. W., Yaszemski, M. J., & Mikos, A. G. (1998) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199824)43:4 · Cited by 380 · see citing works
Pleshko, N., Boskey, A., & Mendelsohn, R. (1991) · Biophysical Journal · doi:10.1016/s0006-3495(91)82113-0 · Cited by 377 · see citing works
Three-dimensional nano-HAp/collagen matrix loading with osteogenic cells in organ culture
Du, C., Cui, F. Z., Zhu, X. D., & de Groot, K. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19990315)44:4 · Cited by 365 · see citing works
Nanofiber Generation of Gelatin–Hydroxyapatite Biomimetics for Guided Tissue Regeneration
Kim, H., Song, J.-H., & Kim, H.-E. (2005) · Advanced Functional Materials · doi:10.1002/adfm.200500116 · Cited by 345 · see citing works
The laser-Raman and infra-red spectra of poly(methyl methacrylate)
Willis, H. A., Zichy, V., & Hendra, P. J. (1969) · Polymer · doi:10.1016/0032-3861(69)90101-3 · Cited by 288 · see citing works
Preparation and characterization of nano‐hydroxyapatite/chitosan composite scaffolds
Kong, L., Gao, Y., Cao, W., et al. (2005) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30414 · Cited by 278 · see citing works
Kim, H., Himeno, T., Kawashita, M., et al. (2004) · Journal of The Royal Society Interface · doi:10.1098/rsif.2004.0003 · Cited by 267 · see citing works
Nanostructured Biocomposite Scaffolds Based on Collagen Coelectrospun with Nanohydroxyapatite
Thomas, V., Dean, D. R., Jose, M. V., Mathew, B., Chowdhury, S., & Vohra, Y. K. (2007) · Biomacromolecules · doi:10.1021/bm060879w · Cited by 260 · see citing works
Fourier transform raman spectroscopy of synthetic and biological calcium phosphates
Sauer, G. R., Zunic, W. B., Durig, J. R., & Wuthier, R. E. (1994) · Calcified Tissue International · doi:10.1007/bf00305529 · Cited by 247 · see citing works
Akbarzadeh, R., & Yousefi, A. (2014) · Journal of Biomedical Materials Research Part B: Applied Biomaterials · doi:10.1002/jbm.b.33101 · Cited by 208 · see citing works
Lao, L., Wang, Y., Zhu, Y., Zhang, Y., & Gao, C. (2011) · Journal of Materials Science Materials in Medicine · doi:10.1007/s10856-011-4374-8 · Cited by 193 · see citing works
Biological and mechanical properties of PMMA-based bioactive bone cements
Mousa, W. F., Kobayashi, M., Shinzato, S., Kamimura, M., Neo, M., Yoshihara, S., & Nakamura, T. (2000) · Biomaterials · doi:10.1016/s0142-9612(00)00097-1 · Cited by 178 · see citing works
Dalby, M. J., Silvio, L. D., Harper, E., et al. (2002) · Biomaterials · doi:10.1016/s0142-9612(01)00139-9 · Cited by 159 · see citing works
On the Setting Properties of Acrylic Bone Cement
MEYER, P. R., Lautenschlager, E. P., & Moore, B. K. (1973) · Journal of Bone and Joint Surgery · doi:10.2106/00004623-197355010-00015 · Cited by 159 · see citing works
Study of nanobiomaterial hydroxyapatite in simulated body fluid: Formation and growth of apatite
Chavan, P. N., Bahir, M. M., Mene, R. U., et al. (2009) · Materials Science and Engineering B · doi:10.1016/j.mseb.2009.11.012 · Cited by 150 · see citing works
Osteoblast adherence and resorption activity of isolated osteoclasts on calcium sulphate hemihydrate
Sidqui, M., Collin, P., Vitté, C., et al. (1995) · Biomaterials · doi:10.1016/0142-9612(95)91048-4 · Cited by 148 · see citing works
Poly(L-lactic acid)/hydroxyapatite hybrid nanofibrous scaffolds prepared by electrospinning
Deng, X., Sui, G., Min-li, Z., Chen, G., & Yang, X. (2006) · Journal of Biomaterials Science Polymer Edition · doi:10.1163/156856207779146123 · Cited by 142 · see citing works
Singh, M. K., Shokuhfar, T., Gracio, J. J. D. A., et al. (2008) · Advanced Functional Materials · doi:10.1002/adfm.200700888 · Cited by 121 · see citing works
Tihan, T. G., Ionita, M. D., Popescu, R., et al. (2009) · Materials Chemistry and Physics · doi:10.1016/j.matchemphys.2009.03.019 · Cited by 113 · see citing works
Crystallization of hydroxyapatite at physiological temperature
Ashok, M., Sundaram, N. M., & Kalkura, S. N. (2003) · Materials Letters · doi:10.1016/s0167-577x(02)01140-0 · Cited by 113 · see citing works
Fabrication of poly-DL-lactide/polyethylene glycol scaffolds using the gas foaming technique
Ji, C., Annabi, N., Hosseinkhani, M., Sivaloganathan, S., & Dehghani, F. (2011) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.09.028 · Cited by 113 · see citing works
Singh, A. (2012) · Bulletin of Materials Science · doi:10.1007/s12034-012-0384-5 · Cited by 108 · see citing works
Recent advances in polymer monoliths for ion-exchange chromatography
Nordborg, A., & Hilder, E. F. (2009) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-009-2636-9 · Cited by 105 · see citing works
Sous, M. (1998) · Biomaterials · doi:10.1016/s0142-9612(98)00118-5 · Cited by 99 · see citing works
Facile fabrication of polycarbonate monolith by non-solvent induced phase separation method
Xin, Y., Fujimoto, T., & Uyama, H. (2012) · Polymer · doi:10.1016/j.polymer.2012.04.029 · Cited by 98 · see citing works
Radha, G., Balakumar, S., Venkatesan, B., et al. (2015) · Materials Science and Engineering C · doi:10.1016/j.msec.2015.01.054 · Cited by 79 · see citing works
Self-citationXu, X., Hai, M., Zhang, Q., et al. (2002) · Journal of Optics A Pure and Applied Optics · doi:10.1088/1464-4258/4/3/303 · Cited by 76 · see citing works
Nasr‐Esfahani, M., & Mehrabanian, M. (2011) · International Journal of Nanomedicine · doi:10.2147/ijn.s21203 · Cited by 76 · see citing works
Hierarchically structured PMMA fibers fabricated by electrospinning
Li, L., Jiang, Z., Li, M., Li, R., & Fang, T. (2014) · RSC Advances · doi:10.1039/c4ra05385k · Cited by 75 · see citing works
Properties of ?-caprolactone/DL-lactide (?-CL/DL-LA) copolymers with a minor ?-CL content
Hiljanen-Vainio, M., Orava, P., & Sepp�l�, J. V. (1997) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(199701)34:1 · Cited by 71 · see citing works
Gong, Y., Ma, Z., Gao, C., et al. (2006) · Journal of Applied Polymer Science · doi:10.1002/app.23931 · Cited by 69 · see citing works
Miyazaki, T., Ohtsuki, C., Kyomoto, M., et al. (2003) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.20042 · Cited by 69 · see citing works
A Novel Composite PMMA-based Bone Cement with Reduced Potential for Thermal Necrosis
Lv, Y., Li, A., Zhou, F., et al. (2015) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.5b01447 · Cited by 67 · see citing works
Yoneda, S., Han, W., Hasegawa, U., et al. (2014) · Polymer · doi:10.1016/j.polymer.2014.05.031 · Cited by 66 · see citing works
Quellec, P., Gref, R., Dellacherie, E., Sommer, F., Tran, M. D., & Alonso, M. J. (1999) · Journal of Biomedical Materials Research · doi:10.1002/(sici)1097-4636(19991205)47:3 · Cited by 64 · see citing works
Oh, S. H., Park, S. C., Kim, H. K., Koh, Y. J., Lee, J.-H., Lee, M. C., & Lee, J. H. (2010) · Journal of Biomaterials Science Polymer Edition · doi:10.1163/092050609x12597621891620 · Cited by 54 · see citing works
Calculation of the Raman line broadening on carbonation in synthetic hydroxyapatite
de Mul, F. F. M., Otto, C., Greve, J. W. M., et al. (1988) · Journal of Raman Spectroscopy · doi:10.1002/jrs.1250190104 · Cited by 47 · see citing works
A New Highly Bioactive Composite for Scaffold Applications: A Feasibility Study
Bellucci, D., Cannillo, V., & Sola, A. (2011) · Materials · doi:10.3390/ma4020339 · Cited by 39 · see citing works
Arabmotlagh, M., Bachmaier, S., Geiger, F., & Rauschmann, M. (2014) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33140 · Cited by 25 · see citing works
Zhang, J., Liao, J., Mo, A., et al. (2008) · Applied Surface Science · doi:10.1016/j.apsusc.2008.06.127 · Cited by 23 · see citing works
Sun, X., & Uyama, H. (2014) · Colloid & Polymer Science · doi:10.1007/s00396-013-3155-y · Cited by 18 · see citing works
Cho, J. S., Lee, J., & Kang, Y. C. (2014) · Physical Chemistry Chemical Physics · doi:10.1039/c4cp01808g · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
Kavitha Sri, A., Arthi, C., Neya, N. R., & Hikku, G. S. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acc99e · Cited by 57 · see citing works
- Authors
- G, R., S, B., Venkatesan, B., & Vellaichamy, E.
- Affiliations
- University of Madras (IN)
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2017-06
- Volume
- 75
- Pages
- 221-228
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W2558084908
- Cited by
- 56
- References
- 50
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 12 · 2023: 12 · 2022: 2 · 2021: 5 · 2020: 7 · 2019: 6 · 2018: 4 · 2017: 2
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Funders
- UGC
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Keywords
- hydroxyapatite nanoparticles, poly(methyl methacrylate), hybrid scaffolds, thermally induced phase separation, wet chemical processing, porous architecture, compressive strength, in vitro bioactivity, apatite layer formation, cell proliferation, hemocompatibility, bone tissue engineering
- MeSH terms
- Cell Line, Humans, Materials Testing, Osteoblasts, Durapatite, Polymethyl Methacrylate, Cell Proliferation, Nanocomposites, Tissue Scaffolds
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
