Dendritic localization of the RNA-binding protein HuD in hippocampal neurons: association with polysomes and upregulation during contextual learning
Abstract
The RNA-binding protein HuD binds to and stabilizes a number of neuronal-specific mRNAs. Recent work from our laboratory indicated that HuD expression is increased in neurons during peripheral nerve regeneration. To gain further insight into the function of this protein in CNS neurons we examined the levels of expression and localization of HuD in hippocampal neurons under normal conditions and in animals subjected to a learning paradigm, contextual fear conditioning (CFC). In the adult hippocampal formation, HuD immunoreactivity was highest in CA3 pyramidal neurons and interneurons in the hilus, moderate in the CA1 region and not detectable in dentate granule cells. Using confocal microscopy we found that HuD immunoreactivity was associated with large cytoplasmic granules in the neuronal cell body and smaller granules in dendrites. Both types of granules were also stained with the ribosomal marker Y10B, suggesting that they also contain ribosomes. Consistent with this idea, subcellular fractionation and immunoprecipitation analyses indicated that HuD is present in both the polysomal (P130) and cytosolic (S130) fraction. In addition to the basal pattern of HuD expression, we examined changes in the levels of this protein 24 h after rats were subjected to a single trial CFC paradigm. HuD protein expression was found to increase in the hilus and CA3 regions of the hippocampus but not in CA1. Our findings suggest that HuD plays a role in synaptic plasticity mechanisms stabilizin…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
HuD protein levels increased in somatic and dendritic compartments of hilar and CA3 hippocampal neurons following contextual fear conditioning.
“Closer inspection of HuD expression revealed increased HuD protein levels in somatic and dendritic compartments of hilar and CA3 hippocampal neurons following a different learning paradigm, contextual fear conditioning (Bolognani et al. 2004).”
From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Bolognani et al. 2004
Cites
Zalfa, F., Giorgi, M., Primerano, B., Moro, A., Di Penta, A., Reis, S., Oostra, B., & Bagni, C. (2003) · Cell · doi:10.1016/s0092-8674(03)00079-5 · Cited by 676 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Pape, H.-C., & Pare, D. (2010) · Physiological Reviews · doi:10.1152/physrev.00037.2009 · Cited by 994 · see citing works
Defining a neuron: neuronal ELAV proteins
Pascale, A., Amadio, M., & Quattrone, A. (2008) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-007-7017-y · Cited by 158 · see citing works
De Rubeis, S., & Bagni, C. (2010) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2009.09.013 · Cited by 126 · see citing works
Neuronal ELAV proteins enhance mRNA stability by a PKCα-dependent pathway
Pascale, A., Amadio, M., Scapagnini, G., Lanni, C., Racchi, M., Provenzani, A., Govoni, S., Alkon, D. L., & Quattrone, A. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504702102 · Cited by 129 · see citing works
Bronicki, L. M., & Jasmin, B. J. (2013) · RNA · doi:10.1261/rna.039164.113 · Cited by 126 · see citing works
A role for the ELAV RNA-binding proteins in neural stem cells: stabilization of<i>Msi1</i>mRNA
Ratti, A., Fallini, C., Cova, L., Fantozzi, R., Calzarossa, C., Zennaro, E., Pascale, A., Quattrone, A., & Silani, V. (2006) · Journal of Cell Science · doi:10.1242/jcs.02852 · Cited by 86 · see citing works
Ratti, A., Fallini, C., Colombrita, C., Pascale, A., Laforenza, U., Quattrone, A., & Silani, V. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m706082200 · Cited by 66 · see citing works
Amadio, M., Pascale, A., Wang, J., Ho, L., Quattrone, A., Gandy, S., Haroutunian, V., Racchi, M., & Pasinetti, G. M. (2009) · Journal of Alzheimer's Disease · doi:10.3233/jad-2009-0967 · Cited by 63 · see citing works
Deschênes-Furry, J., Mousavi, K., Bolognani, F., Neve, R. L., Parks, R. J., Perrone-Bizzozero, N. I., & Jasmin, B. J. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.4626-06.2007 · Cited by 43 · see citing works
Self-citationLee, Y.-S., Choi, S.-L., Jun, H., Yim, S.-J., Lee, J.-A., Kim, H. F., Lee, S.-H., Shim, J., Lee, K., Jang, D.-J., et al. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1116224109 · Cited by 15 · see citing works
- Authors
- Bolognani, F., Merhege, M. A., Twiss, J., & Perrone-Bizzozero, N. I.
- Affiliations
- University of New Mexico (US)
- Alfred I. duPont Hospital for Children (US)
- ORCID
- Twiss, Jeffery
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2004-11
- Volume
- 371
- Issue
- 2-3
- Pages
- 152-157
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W2040890514
- Cited by
- 68
- References
- 1
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 4 · 2017: 3 · 2015: 5 · 2014: 3 · 2013: 6 · 2012: 7
- FWCI
- 1.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (1s10rr014668-01, 5p20rr011830-03)
- National Cancer Institute (R24CA088339, R24CA88339)
- National Institute of Neurological Disorders and Stroke (NS 30255, NS41596, R01NS041596)
- National Center for Research Resources (P20 RR 11830, 1-S10-RR-14668)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- HUD, RNA-binding proteins, dendritic localization, hippocampal neurons, polysomes, contextual fear conditioning, mRNA stabilization, synaptic plasticity, CA3 pyramidal cells, ribosome, hippocampal formation
- MeSH terms
- ELAV-Like Protein 4, Animals, Cerebral Cortex, Dendrites, Hippocampus, Learning, Nerve Tissue Proteins, Neurons, Polyribosomes, Up-Regulation, RNA-Binding Proteins, Mice, Rats, ELAV Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
