Cited by 15RNA Research and SplicingNeuroscience and Neuropharmacology ResearchMemory and Neural Mechanisms

AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic plasticity in Aplysia

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Lee, Yong-Seok, Choi, Sun-Lim, Jun, Heejung, Yim, Se-Jeong, Lee, Jin-A, Kim, Hyoung F., Lee, Seung-Hee, Shim, Jaehoon +3 more·2012·Proceedings of the National Academy of Sciences

Abstract

The consolidation of long-term memory for sensitization and synaptic facilitation in Aplysia requires synthesis of new mRNA including the immediate early gene Aplysia CCAAT enhancer-binding protein ( ApC/EBP ). After the rapid induction of ApC/EBP expression in response to repeated treatments of 5-hydroxytryptamine (5-HT), ApC/EBP mRNA is temporarily expressed in sensory neurons of sensory-to-motor synapses. However, the molecular mechanism underlying the rapid degradation of ApC/EBP transcript is not known. Here, we cloned an AU-rich element (ARE)-binding protein, ApAUF1, which functions as a destabilizing factor for ApC/EBP mRNA. ApAUF1 was found to bind to the 3′ UTR of ApC/EBP mRNA that contains AREs and subsequently reduces the expression of ApC/EBP 3′ UTR-containing reporter genes. Moreover, overexpression of ApAUF1 inhibited the induction of ApC/EBP mRNA in sensory neurons and also impaired long-term facilitation of sensory-to-motor synapses by repetitive 5-HT treatments. These results provide evidence for a critical role of the posttranscriptional modification of ApC/EBP mRNA during the consolidation of synaptic plasticity.

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  • Shared DATs included several RNAs with known enrichment in the cerebellum and transcripts with AU-rich elements, which could contribute to reduced neuronal health/survival in individuals with PCH1B.

    “However, shared DATs included several RNAs that have known enrichment in the cerebellum as well as enrichment in transcripts with AU-rich elements, that could contribute to reduced neuronal health/survival in individuals with PCH1B in agreement with prior work93,94.”

    From EXOSC3 G191 Variants Trigger System-Wide Recalibration of RNA Processing Machinery (2026) · cites this paper as [93]

    The Discussion (page unavailable in our copy) reports that an AU-rich element-binding protein destabilizes an mRNA involved in synaptic plasticity in Aplysia. It does not establish that the shared transcripts are cerebellum-enriched or that they contribute to reduced neuronal health or survival in people with PCH1B. (LLM, conf=0.83)

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Authors
Lee, Y.-S., Choi, S.-L., Jun, H., Yim, S.-J., Lee, J.-A., Kim, H. F., Lee, S.-H., Shim, J., Lee, K., Jang, D.-J., & Kaang, B.-K.
Affiliations
  • Seoul National University (KR)
  • National Creative Research Initiative Center for Memory, Departments of Biological Sciences and
  • Hannam University (KR)
  • Kyungpook National University (KR)
Venue
Proceedings of the National Academy of Sciences
Published
2012-09-04
Volume
109
Issue
38
Pages
15520-15525
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2061329380
Cited by
15
References
46
Citations by year
2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2021: 1 · 2020: 1 · 2019: 2 · 2015: 3 · 2014: 1
FWCI
0.51
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Seoul National University Open Repository (Seoul National University) (submittedVersion)
Funders
Kyungpook National University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
Keywords
AU-rich element-binding proteins, C/EBP, mRNA stability, AU-rich elements, 3' untranslated region, post-transcriptional regulation, synaptic plasticity, long-term facilitation, serotonin, sensitization, memory consolidation, Aplysia, sensory neurons
MeSH terms
Heterogeneous Nuclear Ribonucleoprotein D0, Animals, Aplysia, Cloning, Molecular, Gene Expression Regulation, Humans, Models, Biological, Models, Genetic, Synaptic Transmission, Neuronal Plasticity, RNA Processing, Post-Transcriptional, RNA, Messenger, In Situ Hybridization, Genes, Reporter, 3' Untranslated Regions, CCAAT-Enhancer-Binding Proteins, Heterogeneous-Nuclear Ribonucleoprotein D, HEK293 Cells

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