AU-rich element-binding protein negatively regulates CCAAT enhancer-binding protein mRNA stability during long-term synaptic plasticity in Aplysia
Open accessAbstract
The consolidation of long-term memory for sensitization and synaptic facilitation in Aplysia requires synthesis of new mRNA including the immediate early gene Aplysia CCAAT enhancer-binding protein ( ApC/EBP ). After the rapid induction of ApC/EBP expression in response to repeated treatments of 5-hydroxytryptamine (5-HT), ApC/EBP mRNA is temporarily expressed in sensory neurons of sensory-to-motor synapses. However, the molecular mechanism underlying the rapid degradation of ApC/EBP transcript is not known. Here, we cloned an AU-rich element (ARE)-binding protein, ApAUF1, which functions as a destabilizing factor for ApC/EBP mRNA. ApAUF1 was found to bind to the 3′ UTR of ApC/EBP mRNA that contains AREs and subsequently reduces the expression of ApC/EBP 3′ UTR-containing reporter genes. Moreover, overexpression of ApAUF1 inhibited the induction of ApC/EBP mRNA in sensory neurons and also impaired long-term facilitation of sensory-to-motor synapses by repetitive 5-HT treatments. These results provide evidence for a critical role of the posttranscriptional modification of ApC/EBP mRNA during the consolidation of synaptic plasticity.
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Shared DATs included several RNAs with known enrichment in the cerebellum and transcripts with AU-rich elements, which could contribute to reduced neuronal health/survival in individuals with PCH1B.
“However, shared DATs included several RNAs that have known enrichment in the cerebellum as well as enrichment in transcripts with AU-rich elements, that could contribute to reduced neuronal health/survival in individuals with PCH1B in agreement with prior work93,94.”
From EXOSC3 G191 Variants Trigger System-Wide Recalibration of RNA Processing Machinery (2026) · cites this paper as [93]
The Discussion (page unavailable in our copy) reports that an AU-rich element-binding protein destabilizes an mRNA involved in synaptic plasticity in Aplysia. It does not establish that the shared transcripts are cerebellum-enriched or that they contribute to reduced neuronal health or survival in people with PCH1B. (LLM, conf=0.83)
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“However, shared DATs included several RNAs that have known enrichment in the cerebellum as well as enrichment in transcripts with AU-rich elements, that could contribute to reduced neuronal health/survival in individuals with PCH1B in agreement with prior work 93,94 .”
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- Authors
- Lee, Y.-S., Choi, S.-L., Jun, H., Yim, S.-J., Lee, J.-A., Kim, H. F., Lee, S.-H., Shim, J., Lee, K., Jang, D.-J., & Kaang, B.-K.
- Affiliations
- Seoul National University (KR)
- National Creative Research Initiative Center for Memory, Departments of Biological Sciences and
- Hannam University (KR)
- Kyungpook National University (KR)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 2012-09-04
- Volume
- 109
- Issue
- 38
- Pages
- 15520-15525
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC3458389
- OpenAlex
- W2061329380
- Cited by
- 15
- References
- 46
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2021: 1 · 2020: 1 · 2019: 2 · 2015: 3 · 2014: 1
- FWCI
- 0.51
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Seoul National University Open Repository (Seoul National University) (submittedVersion)
- Funders
- Kyungpook National University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- AU-rich element-binding proteins, C/EBP, mRNA stability, AU-rich elements, 3' untranslated region, post-transcriptional regulation, synaptic plasticity, long-term facilitation, serotonin, sensitization, memory consolidation, Aplysia, sensory neurons
- MeSH terms
- Heterogeneous Nuclear Ribonucleoprotein D0, Animals, Aplysia, Cloning, Molecular, Gene Expression Regulation, Humans, Models, Biological, Models, Genetic, Synaptic Transmission, Neuronal Plasticity, RNA Processing, Post-Transcriptional, RNA, Messenger, In Situ Hybridization, Genes, Reporter, 3' Untranslated Regions, CCAAT-Enhancer-Binding Proteins, Heterogeneous-Nuclear Ribonucleoprotein D, HEK293 Cells
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