Cited by 43RNA Research and SplicingNerve injury and regenerationNeuroscience and Neuropharmacology Research

The RNA-Binding Protein HuD Binds Acetylcholinesterase mRNA in Neurons and Regulates its Expression after Axotomy

Open access
Deschênes-Furry, Julie, Mousavi, Kambiz, Bolognani, Federico, Neve, Rachael L., Parks, Robin J., Perrone-Bizzozero, Nora I., Jasmin, Bernard J.·2007·The Journal of Neuroscience

Abstract

After axotomy, expression of acetylcholinesterase (AChE) is greatly reduced in the superior cervical ganglion (SCG); however, the molecular events involved in this response remain unknown. Here, we first examined AChE mRNA levels in the brain of transgenic mice that overexpress human HuD. Both in situ hybridization and reverse transcription-PCR demonstrated that AChE transcript levels were increased by more than twofold in the hippocampus of HuD transgenic mice. Additionally, direct interaction between the HuD transgene product and AChE mRNA was observed. Next, we examined the role of HuD in regulating AChE expression in intact and axotomized rat SCG neurons. After axotomy of the adult rat SCG neurons, AChE transcript levels decreased by 50 and 85% by the first and fourth day, respectively. In vitro mRNA decay assays indicated that the decrease in AChE mRNA levels resulted from changes in the stability of presynthesized transcripts. A combination of approaches performed using the region that directly encompasses an adenylate and uridylate (AU)-rich element within the AChE 3'-untranslated region demonstrated a decrease in RNA-protein complexes in response to axotomy of the SCG and, specifically, a decrease in HuD binding. After axotomy, HuD transcript and protein levels also decreased. Using a herpes simplex virus construct containing the human HuD sequence to infect SCG neurons in vivo, we found that AChE and GAP-43 mRNA levels were maintained in the SCG after axotomy. Together, the results of this study demonstrate that AChE expression in neurons of the rat SCG is regulated via post-transcriptional mechanisms that involve the AU-rich element and HuD.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1523/jneurosci.4626-06.2007

Cites

98 of 98 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Between 1 and 4 d following axotomy of superior cervical ganglion (SCG) neurons, HuD protein and mRNA levels, its interaction with AChE mRNA, and AChE transcript levels decreased.

    “A study in our laboratory demonstrated that, between 1 and 4 d following axotomy of superior cervical ganglion (SCG) neurons, HuD protein and mRNA levels, its interaction with AChE mRNA, and AChE transcript levels decreased (Deschenes-Furry et al. 2007).”

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Deschênes-Furry, J., Mousavi, K., Bolognani, F., Neve, R. L., Parks, R. J., Perrone-Bizzozero, N. I., & Jasmin, B. J.
Affiliations
  • University of Ottawa (CA)
  • Harvard University (US)
  • Ottawa Hospital (CA)
Corresponding author
Jasmin, Bernard J.
Venue
The Journal of Neuroscience
Published
2007-01-17
Volume
27
Issue
3
Pages
665-675
ISSN
  • 0270-6474 (ISSN-L)
  • 1529-2401
OpenAlex
W1977621096
Cited by
43
References
98
Citations by year
2026: 4 · 2022: 3 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 4 · 2015: 1 · 2014: 2 · 2013: 5 · 2012: 4
FWCI
1.19
Type
journal article
Language
en
Open access
Locations
  • Journal of Neuroscience (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Muscular Dystrophy Association
  • National Institutes of Health
  • Canadian Institutes of Health Research
  • National Institute of Neurological Disorders and Stroke (NS 30255, R01NS030255)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
HUD, RNA-binding proteins, acetylcholinesterase, mRNA stability, axotomy, superior cervical ganglion, AU-rich elements, post-transcriptional regulation, GAP-43, neuronal gene expression, hippocampus, protein-RNA complex
MeSH terms
ELAV-Like Protein 4, Acetylcholinesterase, Animals, Female, Humans, Mice, Transgenic, Neurons, Protein Binding, RNA, Messenger, Gene Expression Regulation, Enzymologic, RNA-Binding Proteins, Rats, Sprague-Dawley, Superior Cervical Ganglion, Axotomy, Mice, Rats, ELAV Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review