Age-Related Changes in Antioxidant Enzymes Related to Hydrogen Peroxide Metabolism in Rat Inner Ear
Open accessAbstract
Oxidative stress is a pervasive factor in aging and has been implicated in noise-induced cochlear pathology. In this study, we measured the activities of two enzymes that catalyze the removal of hydrogen peroxide (H(2)O(2)), catalase and glutathione peroxidase (Gpx), in 3- and 24-month-old Fisher-344 rats, and reduced and oxidized glutathione in 3-, 12-, and 24-month-old rats. There was an increase in Gpx activity in vascular tissue (spiral ligament and stria vascularis), but no change in modiolar, sensory or vestibular tissue of the cochlea. The elevation in vascular tissue was age-related. We observed a significant elevation of catalase activity in vestibular tissue, a tendency for age-related elevation in the modiolus, but no change in vascular or sensory cochlear tissue. These findings suggest that increased Gpx activity in vascular cochlear tissue may be an age-related compensation for a decrease in glutathione and a decline in the redox state measured by the ratio of reduced to oxidized glutathione.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Aging is a complex process where most antioxidant enzymes, including peroxidases, undergo a marked change.
“Aging is a complex process where most antioxidant enzymes, including peroxidases, undergo a marked change [30].”
From The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence (2022) · cites this paper as [30]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Aebi, H. (1984) · Methods in Enzymology · doi:10.1016/s0076-6879(84)05016-3 · Cited by 25,696 · see citing works
Aging: a theory based on free radical and radiation chemistry
Harman, D. (1956) · Journal of Gerontology · doi:10.1093/geronj/11.3.298 · Cited by 8,567 · see citing works
[12] Assays of glutathione peroxidase
Flohé, L., & Günzler, W. A. (1984) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(84)05015-1 · Cited by 4,954 · see citing works
The Free Radical Theory of Aging Matures
BECKMAN, K. B., & AMES, B. N. (1998) · Physiological Reviews · doi:10.1152/physrev.1998.78.2.547 · Cited by 3,879 · see citing works
Reed, D. J., Babson, J. R., Beatty, P. W., Brodie, A. E., Ellis, W. W., & Potter, D. W. (1980) · Analytical Biochemistry · doi:10.1016/0003-2697(80)90118-9 · Cited by 1,178 · see citing works
Trends in oxidative aging theories
Muller, F. L., Lustgarten, M. S., Jang, Y., Richardson, A., & Van Remmen, H. (2007) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2007.03.034 · Cited by 1,053 · see citing works
Protein S-glutathionylation: a regulatory device from bacteria to humans.
Dalle-Donne, I., Rossi, R., Colombo, G., Giustarini, D., & Milzani, A. (2009) · TIBS -Trends in Biochemical Sciences. Regular ed · doi:10.1016/j.tibs.2008.11.002 · Cited by 640 · see citing works
Free Radical Theory of Aging: An Update: Increasing the Functional Life Span
HARMAN, D. (2006) · Annals of the New York Academy of Sciences · doi:10.1196/annals.1354.003 · Cited by 601 · see citing works
The effects of stress and aging on glutathione metabolism
Maher, P. A. (2005) · Ageing Research Reviews · doi:10.1016/j.arr.2005.02.005 · Cited by 426 · see citing works
Mammalian catalase: a venerable enzyme with new mysteries
Kirkman, H. N., & Gaetani, G. F. (2006) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2006.11.003 · Cited by 411 · see citing works
Junqueira, V. B. C., Barros, S. B. D. M., Chan, S. S., Rodrigues, L., Giavarotti, L., Abud, R. L., & Deucher, G. P. (2004) · Molecular Aspects of Medicine · doi:10.1016/j.mam.2004.02.003 · Cited by 329 · see citing works
Alteration of antioxidant enzymes with aging in rat skeletal muscle and liver
Ji, L., Dillon, D. A. R., & Wu, E. (1990) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.1990.258.4.r918 · Cited by 215 · see citing works
Oxidative imbalance in the aging inner ear
Jiang, H., Talaska, A. E., Schacht, J., & Sha, S. (2006) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2006.06.025 · Cited by 195 · see citing works
Age-related cochlear hair cell loss is enhanced in mice lacking copper/zinc superoxide dismutase
McFadden, S. L., Ding, D., Reaume, A. G., Flood, D. G., & Salvi, R. (1999) · Neurobiology of Aging · doi:10.1016/s0197-4580(99)00018-4 · Cited by 188 · see citing works
Terlecky, S. R., Koepke, J. I., & Walton, P. A. (2006) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2006.08.017 · Cited by 138 · see citing works
Lautermann, J., Crann, S. A., McLaren, J. D., & Schacht, J. (1997) · Hearing Research · doi:10.1016/s0378-5955(97)00154-8 · Cited by 133 · see citing works
Oxidative Stress in Aging in the C57B16/J Mouse Cochlea
Staecker, H. (2001) · Acta Oto-Laryngologica · doi:10.1080/00016480152583593 · Cited by 106 · see citing works
Cochlear degeneration in aged rats of four strains
Keithley, E. M., Ryan, A. F., & Feldman, M. L. (1992) · Hearing Research · doi:10.1016/0378-5955(92)90113-2 · Cited by 104 · see citing works
Modulation of Presbycusis: Current Status and Future Directions
Willott, J. F., Chisolm, T. H., & Lister, J. J. (2001) · Audiology and Neurotology · doi:10.1159/000046129 · Cited by 89 · see citing works
Overexpression of Copper/Zinc- Superoxide Dismutase Protects from Kanamycin-Induced Hearing Loss
Sha, S.-H., Zajic, G., Epstein, C. J., & Schacht, J. (2001) · Audiology and Neurotology · doi:10.1159/000046818 · Cited by 79 · see citing works
Effect of SOD1 overexpression on age- and noise-related hearing loss
Coling, D., Yu, K. C. Y., Somand, D. M., Satar, B., Bai, U., Huang, T., Seidman, M. D., Epstein, C. J., Mhatre, A. N., & Lalwani, A. K. (2003) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(02)01439-9 · Cited by 68 · see citing works
Mitochondrial DNA Deletions Associated With Aging and Presbyacusis
Seidman, M. D., Bai, U., Khan, M. J., & Quirk, W. S. (1997) · Archives of Otolaryngology - Head and Neck Surgery · doi:10.1001/archotol.1997.01900100009001 · Cited by 64 · see citing works
McFadden, S. L., Ding, D., & Salvi, R. (2001) · International Journal of Audiology · doi:10.3109/00206090109073128 · Cited by 61 · see citing works
Anatomical, metabolic and genetic aspects of age-related hearing loss in mice.
McFadden, S. L., Ding, D., & Salvi, R. (2001) · PubMed · Cited by 59 · see citing works
Aging outer hair cells (OHCs) in the Fischer 344 rat cochlea: Function and morphology
Chen, G., Li, M., Tanaka, C., Bielefeld, E. C., Hu, B., Kermany, M. H., Salvi, R., & Henderson, D. (2008) · Hearing Research · doi:10.1016/j.heares.2008.11.010 · Cited by 58 · see citing works
Age-related hearing loss in the Fischer 344/NHsd rat substrain
Bielefeld, E. C., Coling, D., Chen, G., Li, M., Tanaka, C., Hu, B., & Henderson, D. (2008) · Hearing Research · doi:10.1016/j.heares.2008.04.006 · Cited by 58 · see citing works
A new and versatile method for determination of thiolamines of biological importance
Dominick, P. K., Cassidy, P. B., & Roberts, J. C. (2001) · Journal of Chromatography B Biomedical Sciences and Applications · doi:10.1016/s0378-4347(01)00298-5 · Cited by 42 · see citing works
Bielefeld, E. C., Coling, D., Chen, G., & Henderson, D. (2008) · Journal of Negative Results in BioMedicine · doi:10.1186/1477-5751-7-4 · Cited by 35 · see citing works
Elevation of superoxide dismutase increases acoustic trauma from noise exposure
Endo, T., Nakagawa, T., Iguchi, F., Kita, T., Okano, T., Sha, S., Schacht, J., Shiga, A., Kim, T. S., & Ito, J. (2004) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2004.11.008 · Cited by 30 · see citing works
Apoptotic outer hair cell death in the cochleae of aging Fischer 344/NHsd rats
Hu, B. H., Yang, W., Bielefeld, E. C., Li, M., Chen, G., & Henderson, D. (2008) · Hearing Research · doi:10.1016/j.heares.2008.08.006 · Cited by 28 · see citing works
Liu, R.-M., & Dickinson, D. A. (2003) · Antioxidants and Redox Signaling · doi:10.1089/152308603770310176 · Cited by 26 · see citing works
Tanaka, C., Bielefeld, E. C., Chen, G., Li, M., & Henderson, D. (2009) · The Laryngoscope · doi:10.1002/lary.20244 · Cited by 18 · see citing works
Chen, J., & Ruan, R. (2008) · Acta Oto-Laryngologica · doi:10.1080/00016480802311015 · Cited by 11 · see citing works
Flagged first, then by citations
1 of the 34 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Oxidative stress response and Nrf2 signaling in aging
Zhang, H., Davies, K. J. A., & Forman, H. J. (2015) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2015.05.036 · Cited by 804 · see citing works
Current concepts in age-related hearing loss: Epidemiology and mechanistic pathways
Yamasoba, T., Lin, F. R., Someya, S., Kashio, A., Sakamoto, T., & Kondo, K. (2013) · Hearing Research · doi:10.1016/j.heares.2013.01.021 · Cited by 596 · see citing works
Oxidative Stresses and Mitochondrial Dysfunction in Age-Related Hearing Loss
Fujimoto, C., & Yamasoba, T. (2014) · Oxidative Medicine and Cellular Longevity · doi:10.1155/2014/582849 · Cited by 203 · see citing works
Pathogenesis of presbycusis in animal models: A review
Fetoni, A. R., Picciotti, P. M., Paludetti, G., & Troiani, D. (2011) · Experimental Gerontology · doi:10.1016/j.exger.2010.12.003 · Cited by 196 · see citing works
Role of antioxidants in prevention of age-related hearing loss: a review of literature
Tavanai, E., & Mohammadkhani, G. (2016) · European Archives of Oto-Rhino-Laryngology · doi:10.1007/s00405-016-4378-6 · Cited by 151 · see citing works
The Role of Antioxidants in the Interplay between Oxidative Stress and Senescence
Varesi, A., Chirumbolo, S., Campagnoli, L. I. M., Pierella, E., Piccini, G. B., Carrara, A., Ricevuti, G., Scassellati, C., Bonvicini, C., & Pascale, A. (2022) · Antioxidants · doi:10.3390/antiox11071224 · Cited by 145 · see citing works
Ahmed, Z. M., Yousaf, R., Lee, B. C., Khan, S. N., Lee, S., Lee, K. D., Husnaın, T., Rehman, A. U., Bonneux, S., Ansar, M., et al. (2010) · The American Journal of Human Genetics · doi:10.1016/j.ajhg.2010.11.010 · Cited by 118 · see citing works
Mitochondria-Targeted Antioxidants for Treatment of Hearing Loss: A Systematic Review
Fujimoto, C., & Yamasoba, T. (2019) · Antioxidants · doi:10.3390/antiox8040109 · Cited by 108 · see citing works
Tuberous sclerosis complex–mediated mTORC1 overactivation promotes age-related hearing loss
Fu, X., Sun, X., Zhang, L., Jin, Y., Chai, R., Yang, L., Zhang, A., Liu, X., Bai, X., Li, J., et al. (2018) · Journal of Clinical Investigation · doi:10.1172/jci98058 · Cited by 90 · see citing works
Schachtele, S. J., Mutnal, M. B., Schleiss, M. R., & Lokensgard, J. R. (2011) · Journal of NeuroVirology · doi:10.1007/s13365-011-0024-7 · Cited by 89 · see citing works
Chen, X., Zhao, X., Cai, H. “., Sun, H., Hu, Y., Huang, X., Kong, W., & Kong, W. (2017) · Redox Biology · doi:10.1016/j.redox.2017.04.031 · Cited by 60 · see citing works
Age-Related Changes in the Cochlea and Vestibule: Shared Patterns and Processes
Paplou, V. G., Schubert, N. M. A., & Pyott, S. J. (2021) · Frontiers in Neuroscience · doi:10.3389/fnins.2021.680856 · Cited by 59 · see citing works
Molecular aging of the mammalian vestibular system
Brosel, S., Laub, C., Averdam, A., Bender, A., & Elstner, M. (2015) · Ageing Research Reviews · doi:10.1016/j.arr.2015.12.007 · Cited by 55 · see citing works
Immunometabolism and oxidative stress: roles and therapeutic strategies in cancer and aging
Zhou, N.-J., Bao, W.-Q., Zhang, C., Jiang, M., Liang, T.-L., Ma, G.-Y., Liu, L., Pan, H., & Li, R.-Z. (2025) · npj Aging · doi:10.1038/s41514-025-00250-z · Cited by 44 · see citing works
Sahley, T. L., Hammonds, M. D., & Musiek, F. E. (2013) · Brain Research · doi:10.1016/j.brainres.2013.01.006 · Cited by 34 · see citing works
Tanaka, C., Coling, D., Manohar, S., Chen, G., Hu, B. H., Salvi, R., & Henderson, D. (2012) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2011.12.027 · Cited by 34 · see citing works
Blood–Labyrinth Barrier in Health and Diseases: Effect of Hormetic Nutrients
Cosentino, A., Agafonova, A., Modafferi, S., Salinaro, A. T., Scuto, M. C., Maiolino, L., Fritsch, T., Calabrese, E. J., Lupo, G., Anfuso, C. D., et al. (2023) · Antioxidants and Redox Signaling · doi:10.1089/ars.2023.0251 · Cited by 33 · see citing works
Age‐related effects of DHEA on peripheral markers of oxidative stress
Jacob, M., Janner, D. D. R., Jahn, M. P., Kucharski, L. C., Belló‐Klein, A., & Ribeiro, M. F. M. (2009) · Cell Biochemistry and Function · doi:10.1002/cbf.1619 · Cited by 29 · see citing works
Redox imbalance influence in the myocardial Akt activation in aged rats treated with DHEA
Jacob, M., Janner, D. D. R., Araújo, A. S. D. R., Jahn, M. P., Kucharski, L. C., Moraes, T. B., Dutra‐Filho, C. S., Ribeiro, M. F. M., & Belló‐Klein, A. (2010) · Experimental Gerontology · doi:10.1016/j.exger.2010.08.017 · Cited by 24 · see citing works
Environmental exposure to organochlorine pesticides and deficits in cochlear status in children
Sisto, R., Moleti, A., Palkovičová, Ľ., Wimmerová, S., Lancz, K., Tihányi, J., Čonka, K., Šovčíková, E., Hertz‐Picciotto, I., Jusko, T. A., et al. (2015) · Environmental Science and Pollution Research · doi:10.1007/s11356-015-4690-5 · Cited by 24 · see citing works
Localization of sirtuins (SIRT1-7) in the aged mouse inner ear
Takumida, M., Takumida, H., Katagiri, Y., & Anniko, M. (2015) · Acta Oto-Laryngologica · doi:10.3109/00016489.2015.1093172 · Cited by 21 · see citing works
Role of Free Radical Formation in Murine Cytomegalovirus–Induced Hearing Loss
Pecha, P. P., Almishaal, A. A., Mathur, P. D., Hillas, E., Johnson, T., Price, M. S., Haller, T. J., Yang, J., Rajasekaran, N. S., Firpo, M. A., et al. (2020) · Otolaryngology · doi:10.1177/0194599820901485 · Cited by 18 · see citing works
Zhang, J., Wang, N., & Xu, A. (2019) · PeerJ · doi:10.7717/peerj.6856 · Cited by 11 · see citing works
L-Ergothioneine slows the progression of age-related hearing loss in CBA/CaJ mice
Bauer, M. A., Bazard, P., Acosta, A. A., Bangalore, N., Elessaway, L., Thivierge, M., Chellani, M., Zhu, X., Ding, B., Walton, J. P., et al. (2024) · Hearing Research · doi:10.1016/j.heares.2024.109004 · Cited by 10 · see citing works
The Long Story of Ebselen: From about One Century of its Synthesis to Clinical Trials
Nogara, P. A., Pereira, M. E., Oliveira, C. S., Orian, L., & da Rocha, J. B. T. (2023) · Royal Society of Chemistry eBooks · doi:10.1039/bk9781839167386-00567 · Cited by 10 · see citing works
Protective Effects of Silymarin Against Age-Related Hearing Loss in an Aging Rat Model
Tavanai, E., Mohammadkhani, G., Farahani, S., & Jalaie, S. (2018) · Indian Journal of Otolaryngology and Head & Neck Surgery · doi:10.1007/s12070-018-1294-7 · Cited by 9 · see citing works
Ibáñez‐Contreras, A., Hernández-Arciga, U., Poblano, A., Arteaga‐Silva, M., Hernández‐Godínez, B., Mendoza‐Cuevas, G., Toledo-Pérez, R., Alarcón‐Aguilar, A., González‐Puertos, V. Y., & Königsberg, M. (2017) · Experimental Gerontology · doi:10.1016/j.exger.2017.11.003 · Cited by 8 · see citing works
Cellular Mechanisms of Age-Related Hearing Loss
Melgar–Rojas, P., Alvarado, J. C., Fuentes‐Santamaría, V., & Juiz, J. M. (2015) · Oxidative stress in applied basic research and clinical practice · doi:10.1007/978-3-319-13473-4_15 · Cited by 6 · see citing works
Savić, A., Beribaka, M., Novaković, A. R., Vasiljević, L., & Palangetić, M. (2026) · Journal of Insect Science · doi:10.1093/jisesa/ieag020 · Cited by 4 · see citing works
Deafening noise down-regulates dopamine transmission in the hub of the central auditory system
Wilson, P., & Apawu, A. K. (2022) · Neurochemistry International · doi:10.1016/j.neuint.2022.105382 · Cited by 4 · see citing works
Liu, S., Yang, Y., Mao, X., Deng, L., Shuai, C., Yao, Y., Shi, Y., & Yin, Z. (2020) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2020.111292 · Cited by 3 · see citing works
DHEA Treatment Effects on Redox Environment in Skeletal Muscle of Young and Aged Healthy Rats
Jacob, M., Fernandes, R. O., Bonetto, J. H. P., Mendes, R. H., Araújo, A. S. D. R., Belló‐Klein, A., & Ribeiro, M. F. M. (2018) · Current Aging Science · doi:10.2174/1874609811666180803125723 · Cited by 2 · see citing works
Oxidative Stress and Cochlear Damage
Hu, B. H., & Henderson, D. (2014) · Springer eBooks · doi:10.1007/978-3-642-30018-9_172 · Cited by 2 · see citing works
Hasansulama, W., Madiadipoera, T. H. S., Sunarjati, & Garna, H. (2022) · Medical Archives · doi:10.5455/medarh.2022.76.209-214 · Cited by 2 · see citing works
Age-related hearing loss: possible associations with Alzheimer’s disease
Ma, Y., Yang, Y., Zhou, Y.-F., Le, W., & Chai, R. (2024) · Ageing and Neurodegenerative Diseases · doi:10.20517/and.2024.24 · Cited by 1 · see citing works
Interventions to Prevent Age-Related Hearing Loss
Yamasoba, T. (2015) · Oxidative stress in applied basic research and clinical practice · doi:10.1007/978-3-319-13473-4_16 · Cited by 1 · see citing works
(2010) · Current Opinion in Otolaryngology & Head & Neck Surgery · doi:10.1097/moo.0b013e32833f3865 · Cited by 1 · see citing works
Sasongko, S. (2015) · Indonesian Journal of Applied Sciences · doi:10.24198/ijas.v5i1.16651
Sasongko, S., Boesoirie, T. S., Parwati, I., Sastramihardja, H. S., & Bashiruddin, J. (2019) · Asian Journal of Applied Sciences · doi:10.24203/ajas.v7i1.5734
Volоtovska, N. V. (2021) · EUREKA Health Sciences · doi:10.21303/2504-5679.2021.001648
Zarei, A. S., Elahi, A., Abasrashid, N., & Taati, B. (2026) · Nutrire · doi:10.1186/s41110-026-00484-1
- Authors
- Coling, D., Chen, S., Chi, L.-H., Jamesdaniel, S., & Henderson, D.
- Affiliations
- University at Buffalo, State University of New York (US)
- Corresponding author
- Coling, Donald
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2009-10
- Volume
- 464
- Issue
- 1
- Pages
- 22-25
- ISSN
- 0304-3940 (ISSN-L)
- PMC
- PMC2760401
- OpenAlex
- W2063248210
- Cited by
- 46
- References
- 34
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 8 · 2018: 3 · 2017: 2 · 2016: 1 · 2015: 7 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.13
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute on Deafness and Other Communication Disorders (R01DC006862)
- Topics
- Life Sciences › Neuroscience › Sensory Systems › Hearing, Cochlea, Tinnitus, Genetics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- age-related changes, antioxidant enzymes, hydrogen peroxide metabolism, catalase, glutathione peroxidase, oxidative stress, cochlea, spiral ligament, stria vascularis, glutathione redox state, F344 rats
- MeSH terms
- Aging, Animals, Catalase, Cochlea, Glutathione Peroxidase, Hydrogen Peroxide, Male, Oxidation-Reduction, Rats, Inbred F344, Stria Vascularis, Glutathione Disulfide, Rats, Spiral Ligament of Cochlea
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
