Cited by 321Nerve injury and regenerationNeurogenesis and neuroplasticity mechanismsIon channel regulation and function

Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier

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Eshed, Yael, Feinberg, Konstantin, Poliak, Sebastian, Sabanay, Helena, Sarig-Nadir, Offra, Spiegel, Ivo, Bermingham, John R., Peles, Elior·2005·Neuron

Abstract

Accumulation of Na(+) channels at the nodes of Ranvier is a prerequisite for saltatory conduction. In peripheral nerves, clustering of these channels along the axolemma is regulated by myelinating Schwann cells through a yet unknown mechanism. We report the identification of gliomedin, a glial ligand for neurofascin and NrCAM, two axonal immunoglobulin cell adhesion molecules that are associated with Na+ channels at the nodes of Ranvier. Gliomedin is expressed by myelinating Schwann cells and accumulates at the edges of each myelin segment during development, where it aligns with the forming nodes. Eliminating the expression of gliomedin by RNAi, or the addition of a soluble extracellular domain of neurofascin to myelinating cultures, which caused the redistribution of gliomedin along the internodes, abolished node formation. Furthermore, a soluble gliomedin induced nodal-like clusters of Na+ channels in the absence of Schwann cells. We propose that gliomedin provides a glial cue for the formation of peripheral nodes of Ranvier.

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Crossref · 10.1016/j.neuron.2005.06.026

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Authors
Eshed, Y., Feinberg, K., Poliak, S., Sabanay, H., Sarig-Nadir, O., Spiegel, I., Bermingham, J. R., & Peles, E.
Affiliations
  • Weizmann Institute of Science (IL)
  • McLaughlin Research Institute (US)
Corresponding author
Peles, Elior
Venue
Neuron
Publisher
Elsevier BV
Published
2005-07
Volume
47
Issue
2
Pages
215-229
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2017281603
Cited by
321
References
70
Citations by year
2026: 4 · 2025: 8 · 2024: 12 · 2023: 8 · 2022: 10 · 2021: 15 · 2020: 13 · 2019: 13 · 2018: 12 · 2017: 16 · 2016: 10 · 2015: 15 · 2014: 27 · 2013: 16 · 2012: 17
FWCI
9.51
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (NS50220)
  • National Multiple Sclerosis Society (RG3594-A-4)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
Schwann cells, nodes of Ranvier, sodium channel clustering, saltatory conduction, neurofascin, NrCAM, axon-glia interaction, myelination, peripheral nervous system development, myelin
MeSH terms
Ezrin, Age Factors, Amino Acid Sequence, Animals, Axons, Cell Compartmentation, Cells, Cultured, Chlorocebus aethiops, Cloning, Molecular, Cytoskeletal Proteins, Myelin Basic Protein, Fluorescent Antibody Technique, Ganglia, Spinal, Humans, Membrane Proteins, Microfilament Proteins, S100 Proteins, Phosphoproteins, Protein Binding, Ranvier's Nodes, Schwann Cells, Sciatic Nerve, Spectrin, Transfection, Blotting, Northern, Blotting, Western, Sodium Channels, Cell Adhesion Molecules, Cell Adhesion Molecules, Neuronal, Microscopy, Immunoelectron, Neurofilament Proteins, Protein Structure, Tertiary, Ankyrins, Receptors, Peptide, Gene Expression Regulation, Developmental, Myelin-Associated Glycoprotein, Macromolecular Substances, Rats, Claudins

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