Activity induced changes in the distribution of Shanks at hippocampal synapses
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Dendritic spines contain a family of abundant scaffolding proteins known as Shanks, but little is known about how their distributions might change during synaptic activity. Here, pre-embedding immunogold electron microscopy is used to localize Shanks in synapses from cultured hippocampal neurons. We find that Shanks are preferentially located at postsynaptic densities (PSDs) as well as in a filamentous network near the PSD, extending up to 120 nm from the postsynaptic membrane. Application of sub-type specific antibodies shows that Shank2 is typically concentrated at and near PSDs while Shank1 is, in addition, distributed throughout the spine head. Depolarization with high K+ for 2 min causes transient, reversible translocation of Shanks towards the PSD that is dependent on extracellular Ca2+. The amount of activity-induced redistribution and subsequent recovery is pronounced for Shank1 but less so for Shank2. Thus, Shank1 appears to be a dynamic element within the spine, whose translocation could be involved in activity-induced, transient structural changes, while Shank2 appears to be a more stable element positioned at the interface of the PSD with the spine cytoplasm.
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Shank3 shares a similar protein domain structure but has a different expression pattern and subcellular localization than Shank1 and Shank2.
“Shank3 shares a similar protein domain structure but has a different expression pattern and subcellular localization than Shank1 and Shank2 (Bockers et al., 2004; Peca et al., 2011; Tao-Cheng et al., 2010).”
From Modeling Autism by SHANK Gene Mutations in Mice (2013) · cites this paper as Tao-Cheng et al., 2010
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- Authors
- Tao-Cheng, J. H., Dosemeci, A., Gallant, P. E., Smith, C., & Reese, T.
- Affiliations
- National Institutes of Health (US)
- Venue
- Neuroscience
- Publisher
- Elsevier BV
- Published
- 2010-06
- Volume
- 168
- Issue
- 1
- Pages
- 11-17
- ISSN
- 0306-4522 (ISSN-L)
- PMC
- PMC2871978
- OpenAlex
- W2048301500
- Cited by
- 61
- References
- 33
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 3 · 2021: 6 · 2020: 13 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 8 · 2014: 4 · 2013: 3 · 2012: 6
- FWCI
- 1.42
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Neuroscience (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Intramural Research Program of the NIH, NINDS
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetics and Neurodevelopmental Disorders
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- shank, Shank1, Shank2, dendritic spines, postsynaptic density, synaptic scaffolding proteins, activity-dependent synaptic plasticity, depolarization, hippocampal neurons, synaptic ultrastructure
- MeSH terms
- Animals, Calcium, Cells, Cultured, Hippocampus, Male, Membrane Proteins, Mice, Inbred C57BL, Nerve Tissue Proteins, Neurons, Synapses, Rats, Sprague-Dawley, Protein Transport, Adaptor Proteins, Signal Transducing, Dendritic Spines, Mice, Rats
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