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Cited by 223Drug Transport and Resistance MechanismsPregnancy and preeclampsia studiesPregnancy and Medication Impact

Expression of the Multidrug Resistance P-Glycoprotein, (ABCB1 glycoprotein) in the Human Placenta Decreases with Advancing Gestation

Sun, M., Kingdom, J., Baczyk, D., Lye, S.J., Matthews, S.G., Gibb, W.·2006·Placenta
DOI 10.1016/j.placenta.2005.05.007
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Abstract

The multidrug resistance p-glycoprotein (P-gp), encoded by the ABCB1 gene, is a plasma membrane protein that actively extrudes a wide variety of substances from cells. Preliminary studies in mice have shown that the ABCB1/P-gp can protect the fetus from a number of toxic substances. ABCB1/P-gp is expressed in the human placenta and is potentially capable of protecting the fetus from a large number of drugs and toxins, including herbicides and pesticides. The protein can also extrude various steroids including certain glucocorticoids and may therefore play an important role in regulating fetal access of glucocorticoids. The aim of the present study was to examine the expression profile and cellular localization of ABCB1/P-gp in human placenta throughout gestation. We hypothesized that there would be gestational age-related changes in the expression of the protein. ABCB1/P-gp mRNA was measured by Real-Time PCR using specific probes in tissues obtained from 6 weeks gestation to term. ABCB1/P-gp mRNA levels in placental tissue obtained at 6-10 weeks (n=5) and 24-35 weeks (n=5) were significantly higher than in tissues obtained at term (38-41 weeks gestation) by elective C-section (n=6) or following labor (n=6). The profile of ABCB1/P-gp protein levels, quantified using Western analysis, demonstrated a similar decrease with advancing gestation. At all gestational ages ABCB1/P-gp was localized by immunohistochemistry to the syncytiotrophoblast. In term tissues, it appeared to be localized to some areas of the villi and not others. Together, these data indicate that with advancing gestation there is a decrease in the level of ABCB1/P-gp in the human placenta indicating that the fetus may be more susceptible to toxic insults in the latter part of gestation. Further, the reduction in ABCB1/P-gp expression may contribute to the increased transfer of maternal cortisol to the fetus that is known to occur in late gestation.

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Crossref · 10.1016/j.placenta.2005.05.007

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  • The mRNA and protein levels of P-glycoprotein reduce across gestation, suggesting the fetus may be more exposed to toxic insults later in pregnancy.

    “The mRNA and protein levels of P-glycoprotein reduce across gestation, suggesting the fetus may be more exposed to toxic insults later in pregnancy (519).”

    From Placental Origins of Chronic Disease (2016) · cites this paper as [519]

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Authors
Sun, M., Kingdom, J., Baczyk, D., Lye, S. J., Matthews, S. G., & Gibb, W.
Affiliations
  • University of Ottawa (CA)
  • Mount Sinai Hospital (CA)
Corresponding author
Gibb, W.
ORCID
Venue
Placenta
Publisher
Elsevier BV
Published
2006-06
Volume
27
Issue
6-7
Pages
602-609
ISSN
0143-4004 (ISSN-L)
DOI
10.1016/j.placenta.2005.05.007
OpenAlex
W2021031411
Cited by
223
References
38
Citations by year
2026: 4 · 2024: 3 · 2023: 9 · 2022: 16 · 2021: 14 · 2020: 13 · 2019: 11 · 2018: 16 · 2017: 10 · 2016: 8 · 2015: 13 · 2014: 12 · 2013: 20 · 2012: 17
FWCI
3.89
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Placenta (publishedVersion)
  • PubMed (publishedVersion)
  • UWA Profiles and Research Repository (UWA) (submittedVersion)
Funders
Canadian Institutes of Health Research
Topics
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
  • Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Pregnancy and Medication Impact
Keywords
P-glycoprotein, multidrug resistance protein, human placenta, gestational age, syncytiotrophoblast
MeSH terms
Adult, Female, Gestational Age, Humans, Maternal-Fetal Exchange, Placenta, Pregnancy, RNA, Messenger, Blotting, Western, Drug Resistance, Multiple, Gene Expression Regulation, Developmental, Reverse Transcriptase Polymerase Chain Reaction, ATP Binding Cassette Transporter, Subfamily B, Member 1

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