Lipid profiling of polarized human monocyte-derived macrophages
Open accessAbstract
The highly orchestrated transcriptional and metabolic reprogramming during activation drastically transforms the main functions and physiology of human macrophages across the polarization spectrum. Lipids, for example, can modify protein function by acting remotely as signaling molecules but also locally by altering the physical properties of cellular membranes. These changes play key roles in the functions of highly plastic immune cells due to their involvement in inflammation, immune responses, phagocytosis and wound healing processes. We report an analysis of major membrane lipids of distinct phenotypes of resting (M0), classically activated (M1), alternatively activated (M2a) and deactivated (M2c) human monocyte derived macrophages from different donors. Samples were subjected to supercritical fluid chromatography-ion mobility-mass spectrometry analysis, which allowed separations based on lipid class, facilitating the profiling of their fatty acid composition. Different levels of arachidonic acid mobilization as well as other fatty acid changes were observed for different lipid classes in the distinct polarization phenotypes, suggesting the activation of highly orchestrated and specific enzymatic processes in the biosynthesis of lipid signaling molecules and cell membrane remodeling. Thromboxane A2 production appeared to be a specific marker of M1 polarization. These alterations to the global composition of lipid bi-layer membranes in the cell provide a potential methodology for the definition and determination of cellular and tissue activation states.
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Altered lipid metabolism and extrinsic supplementation with various lipid substrates have a major impact on microglial reprogramming.
“Altered lipid metabolism and extrinsic supplementation with various lipid substrates both have a major impact on microglial reprogramming [60,61,62,63,64].”
From Lipoprotein Lipase Regulates Microglial Lipid Droplet Accumulation (2021) · cites this paper as [62]
The Discussion reports significant lipid changes associated with polarization of human monocyte-derived macrophages. It does not establish that altered lipid metabolism or supplementation with lipid substrates has a major impact on microglial reprogramming: the studied cells were macrophages, and the Methods describe cytokines and LPS—not lipid substrates—as the polarizing treatments. Page numbers were not supplied in our copy. (LLM, conf=0.85)
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- Authors
- Montenegro-Burke, J. R., Sutton, J. A., Rogers, L. M., Milne, G. L., McLean, J. A., & Aronoff, D. M.
- Affiliations
- Vanderbilt University (US)
- Meharry Medical College (US)
- Vanderbilt University Medical Center (US)
- Corresponding author
- Aronoff, David M.
- Venue
- Prostaglandins & Other Lipid Mediators
- Publisher
- Elsevier BV
- Published
- 2016-12
- Volume
- 127
- Pages
- 1-8
- ISSN
- 1098-8823 (ISSN-L)
- PMC
- PMC6053630
- OpenAlex
- W2550662942
- Cited by
- 44
- References
- 63
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 4 · 2023: 2 · 2022: 6 · 2021: 6 · 2020: 7 · 2019: 3 · 2018: 8
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Prostaglandins & Other Lipid Mediators (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH NIAID Training (5T32HL007737-20)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Sphingolipid Metabolism and Signaling
- Keywords
- macrophage polarization, monocyte-derived macrophages, fatty acid composition, arachidonic acid mobilization, thromboxane A2, M1 macrophages, M2 macrophages, lipid signaling, membrane remodeling
- MeSH terms
- Adolescent, Adult, Fatty Acids, Humans, Macrophages, Membrane Lipids, Middle Aged, Monocytes, Lipid Metabolism, Young Adult
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