Polyphenol fraction of extra virgin olive oil protects against endothelial dysfunction induced by high glucose and free fatty acids through modulation of nitric oxide and endothelin-1
Open accessAbstract
Epidemiological and clinical studies have reported that olive oil reduces the incidence of cardiovascular disease. However, the mechanisms involved in this beneficial effect have not been delineated. The endothelium plays an important role in blood pressure regulation through the release of potent vasodilator and vasoconstrictor agents such as nitric oxide (NO) and endothelin-1 (ET-1), respectively, events that are disrupted in type 2 diabetes. Extra virgin olive oil contains polyphenols, compounds that exert a biological action on endothelial function. This study analyzes the effects of olive oil polyphenols on endothelial dysfunction using an in vitro model that simulates the conditions of type 2 diabetes. Our findings show that high glucose and linoleic and oleic acids decrease endothelial NO synthase phosphorylation, and consequently intracellular NO levels, and increase ET-1 synthesis by ECV304 cells. These effects may be related to the stimulation of reactive oxygen species production in these experimental conditions. Hydroxytyrosol and the polyphenol extract from extra virgin olive oil partially reversed the above events. Moreover, we observed that high glucose and free fatty acids reduced NO and increased ET-1 levels induced by acetylcholine through the modulation of intracellular calcium concentrations and endothelial NO synthase phosphorylation, events also reverted by hydroxytyrosol and polyphenol extract. Thus, our results suggest a protective effect of olive oil polyphenols on endothelial dysfunction induced by hyperglycemia and free fatty acids.
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These effects were related to positive modulation of insulin signaling, which via PI3K/Akt is a critical regulator of eNOS phosphorylation and activity.
“These effects were related to a positive modulation of insulin signaling, that, via PI3K/Akt, is considered a critical regulator of eNOS phosphorylation and activity [199].”
From Mediterranean diet nutrients to turn the tide against insulin resistance and related diseases (2020) · cites this paper as [199]
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- Authors
- Storniolo, C. E., Roselló-Catafau, J., Pintó, X., Mitjavila, M. T., & Moreno, J. J.
- Affiliations
- Universitat de Barcelona (ES)
- Bellvitge University Hospital (ES)
- Venue
- Redox Biology
- Publisher
- Elsevier BV
- Published
- 2014
- Volume
- 2
- Pages
- 971-977
- ISSN
- 2213-2317 (ISSN-L)
- PMC
- PMC4215463
- OpenAlex
- W2046300274
- Cited by
- 121
- References
- 51
- Citations by year
- 2026: 9 · 2025: 7 · 2024: 6 · 2023: 11 · 2022: 7 · 2021: 11 · 2020: 14 · 2019: 17 · 2018: 14 · 2017: 7 · 2016: 7 · 2015: 11
- FWCI
- 5.56
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Redox Biology (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Dipòsit Digital de la Universitat de Barcelona (Universitat de Barcelona) (publishedVersion, cc-by-nc-nd)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Spanish Ministry of Health (RD06/0045)
- Fondo Europeo de Desarrollo Regional
- Spanish Ministry of Science (BFU2007-61727/BFI)
- Generalitat de Catalunya (2009SGR00438)
- Topics
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- extra virgin olive oil, polyphenols, endothelial dysfunction, nitric oxide, endothelin-1, type 2 diabetes, hyperglycemia, free fatty acids, linoleic acid, oleic acid, hydroxytyrosol, reactive oxygen species, intracellular calcium, acetylcholine, cardioprotection
- MeSH terms
- Olive Oil, Cell Line, Diabetes Mellitus, Type 2, Fatty Acids, Nonesterified, Glucose, Nitric Oxide, Oleic Acid, Endothelin-1, Linoleic Acid, Endothelial Cells, Nitric Oxide Synthase Type III, Polyphenols
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