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Cited by 77Effects and risks of endocrine disrupting chemicalsPhytoestrogen effects and researchEstrogen and related hormone effects

Overlapping but distinct effects of genistein and ethinyl estradiol (EE2) in female Sprague–Dawley rats in multigenerational reproductive and chronic toxicity studies

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Delclos, K. Barry, Weis, Constance C., Bucci, Thomas J., Olson, Greg, Mellick, Paul, Sadovova, Natalya, Latendresse, John R., Thorn, Brett +1 more·2009·Reproductive Toxicology
DOI 10.1016/j.reprotox.2008.12.005PMC PMC2706590
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Abstract

Genistein and ethinyl estradiol (EE(2)) were examined in multigenerational reproductive and chronic toxicity studies that had different treatment intervals among generations. Sprague-Dawley rats received genistein (0, 5, 100, or 500 ppm) or EE(2) (0, 2, 10, or 50 ppb) in a low phytoestrogen diet. Nonneoplastic effects in females are summarized here. Genistein at 500 ppm and EE(2) at 50 ppb produced similar effects in continuously exposed rats, including decreased body weights, accelerated vaginal opening, and altered estrous cycles in young animals. At the high dose, anogenital distance was subtly affected by both compounds, and a reduction in litter size was evident in genistein-treated animals. Genistein at 500 ppm induced an early onset of aberrant cycles relative to controls in the chronic studies. EE(2) significantly increased the incidence of uterine lesions (atypical focal hyperplasia and squamous metaplasia). These compound-specific effects appeared to be enhanced in the offspring of prior exposed generations.

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Cited by

Sorted by citations

Authors
Delclos, K. B., Weis, C. C., Bucci, T. J., Olson, G., Mellick, P., Sadovova, N., Latendresse, J. R., Thorn, B., & Newbold, R. R.
Affiliations
  • National Center for Toxicological Research (US)
  • National Institute of Environmental Health Sciences (US)
Corresponding author
Delclos, K. Barry
ORCID
Delclos, K. Barry
Venue
Reproductive Toxicology
Publisher
Elsevier BV
Published
2009-04
Volume
27
Issue
2
Pages
117-132
ISSN
0890-6238 (ISSN-L)
DOI
10.1016/j.reprotox.2008.12.005
PMC
PMC2706590
OpenAlex
W2022165854
Cited by
77
References
3
Citations by year
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FWCI
3.69
Type
journal article
Language
en
Open access
Licence
Locations
  • Reproductive Toxicology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Phytoestrogen effects and research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
genistein, ethinyl estradiol, phytoestrogens, chronic toxicity, female reproductive tract, estrous cycle, vaginal opening, anogenital distance, litter size, uterine lesions, squamous metaplasia, offspring effects, treatment interval
MeSH terms
Animals, Body Weight, Dose-Response Relationship, Drug, Eating, Endometrial Hyperplasia, Estrus, Ethinyl Estradiol, Female, Litter Size, Metaplasia, Pregnancy, Reproduction, Sexual Behavior, Animal, Sexual Maturation, Time Factors, Uterus, Vagina, Rats, Sprague-Dawley, Genistein, Rats, Endocrine Disruptors

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