The DOI matches its Crossref record; 49 of the 50 references it cites were found in a registry or an index.
Human Induced Pluripotent Stem Cell-Derived Macrophages Share Ontogeny with MYB-Independent Tissue-Resident Macrophages
Open accessAbstract
Tissue-resident macrophages, such as microglia, Kupffer cells, and Langerhans cells, derive from Myb-independent yolk sac (YS) progenitors generated before the emergence of hematopoietic stem cells (HSCs). Myb-independent YS-derived resident macrophages self-renew locally, independently of circulating monocytes and HSCs. In contrast, adult blood monocytes, as well as infiltrating, gut, and dermal macrophages, derive from Myb-dependent HSCs. These findings are derived from the mouse, using gene knockouts and lineage tracing, but their applicability to human development has not been formally demonstrated. Here, we use human induced pluripotent stem cells (iPSCs) as a tool to model human hematopoietic development. By using a CRISPR-Cas9 knockout strategy, we show that human iPSC-derived monocytes/macrophages develop in an MYB-independent, RUNX1-, and SPI1 (PU.1)-dependent fashion. This result makes human iPSC-derived macrophages developmentally related to and a good model for MYB-independent tissue-resident macrophages, such as alveolar and kidney macrophages, microglia, Kupffer cells, and Langerhans cells.
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“These cells closely resemble human foetal microglia with regards to transcriptional signature and express high levels of microglial genes29,30.”
From PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages (2021) · cites this paper as [30]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
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“We have recently directly demonstrated that these derive from MYB-independent, RUNX1- and PU.1-dependent precursors, characteristic of yolk sac-derived macrophages (Buchrieser et al., 2017, Vanhee et al., 2015).”
From A highly efficient human pluripotent stem cell microglia model displays a neuronal-co-culture-specific expression profile and inflammatory response (2017) · cites this paper as Buchrieser et al., 2017
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“However, only our protocol has direct evidence by gene knockout for producing MYB-independent primitive macrophages (Buchrieser et al., 2017).”
From A highly efficient human pluripotent stem cell microglia model displays a neuronal-co-culture-specific expression profile and inflammatory response (2017) · cites this paper as Buchrieser et al., 2017
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“Our own work (Buchrieser et al., 2017) and that of others (Hoeffel et al., 2015) indicates that this protocol produces MYB-independent myeloid cells, recapitulating an embryonic ontogeny, and lineage-tracking studies in mouse have demonstrated that microglia derive from primitive, yolk sac macrophages that migrate into the developing brain (Ginhoux et al., 2010, Ginhoux et al., 2013).”
From A highly efficient human pluripotent stem cell microglia model displays a neuronal-co-culture-specific expression profile and inflammatory response (2017) · cites this paper as Buchrieser et al., 2017
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Cites
Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages
Ginhoux, F., Greter, M., Leboeuf, M., Nandi, S., See, P., Gokhan, S., Mehler, M. F., Conway, S. J., Ng, L. G., Stanley, E. R., et al. (2010) · Science · doi:10.1126/science.1194637 · Cited by 5,175
A Lineage of Myeloid Cells Independent of Myb and Hematopoietic Stem Cells
Schulz, C., Perdiguero, E. G., Chorro, L., Szabo-Rogers, H., Cagnard, N., Kierdorf, K., Prinz, M., Wu, B., Jacobsen, S. E. W., Pollard, J. W., et al. (2012) · Science · doi:10.1126/science.1219179 · Cited by 2,578
Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors
Gomez Perdiguero, E., Klapproth, K., Schulz, C., Busch, K., Azzoni, E., Crozet, L., Garner, H., Trouillet, C., de Bruijn, M. F., Geissmann, F., et al. (2015) · Nature · doi:10.1038/nature13989 · Cited by 2,339 · cited 3×
“Murine YS-derived macrophages and fetal monocytes seed most tissues before HSC-derived definitive hematopoiesis (Ginhoux et al., 334 Stem Cell Reports j Vol. 8 j 334–345 j February 14, 2017 j ª 2017 The A This is an open access article under the CC BY license (http://creativecommo 2010; Hoeffel et al., 2015; Kierdorf…”
Hashimoto, D., Chow, A., Noizat, C., Teo, P., Beasley, M. B., Leboeuf, M., Becker, C. D., See, P., Price, J., Lucas, D., et al. (2013) · Immunity · doi:10.1016/j.immuni.2013.04.004 · Cited by 2,311 · cited 2×
“…et al., 2015) where, with the exception of dermal (Tamoutounour et al., 2013), gut (Bain et al., 2014), and a fraction of cardiac macrophages (Epelman et al., 2014), they self-renew throughout life with minor contribution from adult blood monocytes (Epelman et al., 2014; Hashimoto et al., 2013).”
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Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages
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Epelman, S., Lavine, K. J., Beaudin, A. E., Sojka, D. K., Carrero, J. A., Calderon, B., Brija, T., Gautier, E. L., Ivanov, S., Satpathy, A. T., et al. (2014) · Immunity · doi:10.1016/j.immuni.2013.11.019 · Cited by 1,494
Microglia emerge from erythromyeloid precursors via Pu.1- and Irf8-dependent pathways
Kierdorf, K., Erny, D., Goldmann, T., Sander, V., Schulz, C., Perdiguero, E. G., Wieghofer, P., Heinrich, A., Riemke, P., Hölscher, C., et al. (2013) · Nature Neuroscience · doi:10.1038/nn.3318 · Cited by 1,457
Bain, C. C., Bravo-Blas, A., Scott, C. L., Gomez Perdiguero, E., Geissmann, F., Henri, S., Malissen, B., Osborne, L. C., Artis, D., & Mowat, A. M. (2014) · Nature Immunology · doi:10.1038/ni.2967 · Cited by 1,178
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Haenseler, W., Sansom, S. N., Buchrieser, J., Newey, S. E., Moore, C. S., Nicholls, F. J., Chintawar, S., Schnell, C., Antel, J. P., Allen, N. D., et al. (2017) · Stem Cell Reports · doi:10.1016/j.stemcr.2017.05.017 · Cited by 593
Self-citationModeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids
Ouchi, R., Togo, S., Kimura, M., Shinozawa, T., Koido, M., Koike, H., Thompson, W., Karns, R. A., Mayhew, C. N., McGrath, P. S., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.05.007 · Cited by 537
Xu, R., Boreland, A. J., Li, X., Erickson, C., Jin, M., Atkins, C., Pang, Z. P., Daniels, B. P., & Jiang, P. (2021) · Stem Cell Reports · doi:10.1016/j.stemcr.2021.06.011 · Cited by 229
Malainou, C., Abdin, S. M., Lachmann, N., Matt, U., & Herold, S. (2023) · Journal of Clinical Investigation · doi:10.1172/jci170501 · Cited by 215
Xu, R., Li, X., Boreland, A. J., Posyton, A., Kwan, K. Y., Hart, R. P., & Jiang, P. (2020) · Nature Communications · doi:10.1038/s41467-020-15411-9 · Cited by 200
Metadata
- Authors
- Buchrieser, J., James, W., & Moore, M. D.
- Affiliations
- University of Oxford (GB)
- Corresponding author
- Buchrieser, Julian
- Venue
- Stem Cell Reports
- Publisher
- Elsevier BV
- Published
- 2017-02
- Volume
- 8
- Issue
- 2
- Pages
- 334-345
- ISSN
- 2213-6711 (ISSN-L)
- PMC
- PMC5312255
- OpenAlex
- W2575042032
- Cited by
- 215
- References
- 50
- Citations by year
- 2026: 24 · 2025: 18 · 2024: 27 · 2023: 19 · 2022: 25 · 2021: 26 · 2020: 30 · 2019: 15 · 2018: 22 · 2017: 9
- FWCI
- 8.49
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Stem Cell Reports (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Parkinson's UK (2581970, SC037554)
- Research Councils UK
- Wellcome Trust (WTISSF121302)
- National Institute for Health and Care Research (090532/Z/09/Z.)
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Keywords
- tissue-resident macrophages, iPSC-derived macrophages, macrophage ontogeny, hematopoietic development, RUNX1, yolk sac macrophages, microglia, Kupffer cells, Langerhans cells, alveolar macrophages, kidney macrophages, monocytes, CRISPR/Cas9 knockout
- MeSH terms
- Proto-Oncogene Protein Spi-1, Cell Differentiation, Cell Line, Erythrocytes, Granulocytes, Hematopoietic Stem Cells, Humans, Macrophages, Monocytes, Organ Specificity, Phenotype, Proto-Oncogene Proteins, Biomarkers, Trans-Activators, Gene Expression, Gene Expression Regulation, Developmental, Genes, myb, Core Binding Factor Alpha 2 Subunit, Gene Knockout Techniques, Induced Pluripotent Stem Cells, Embryoid Bodies
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