Cited by 71Bacterial Genetics and BiotechnologyRNA and protein synthesis mechanismsLipid Membrane Structure and Behavior

A YidC-like protein in the archaeal plasma membrane.

Open access
Borowska, Marta T., Dominik, Pawel K., Anghel, S. Andrei, Kossiakoff, Anthony A., Keenan, Robert J.·2015·Structure

Abstract

Cells possess specialized machinery to direct the insertion of membrane proteins into the lipid bilayer. In bacteria, the essential protein YidC inserts certain proteins into the plasma membrane, and eukaryotic orthologs are present in the mitochondrial inner membrane and the chloroplast thylakoid membrane. The existence of homologous insertases in archaea has been proposed based on phylogenetic analysis. However, limited sequence identity, distinct architecture, and the absence of experimental data have made this assignment ambiguous. Here we describe the 3.5-Å crystal structure of an archaeal DUF106 protein from Methanocaldococcus jannaschii (Mj0480), revealing a lipid-exposed hydrophilic surface presented by a conserved YidC-like fold. Functional analysis reveals selective binding of Mj0480 to ribosomes displaying a stalled YidC substrate, and a direct interaction between the buried hydrophilic surface of Mj0480 and the nascent chain. These data provide direct experimental evidence that the archaeal DUF106 proteins are YidC/Oxa1/Alb3-like insertases of the archaeal plasma membrane.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.str.2015.06.025

Cites

68 of 68 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • YidC belongs to a conserved group of proteins with homologues in mitochondria, chloroplasts, the endoplasmic reticulum and archaea.

    “It belongs to a conserved group of proteins with homologues in mitochondria, chloroplasts, the endoplasmic reticulum and archaea (Borowska et al., 2015; Anghel et al., 2017; Kuhn and Kiefer, 2017; McDowell et al., 2021).”

    From The Dynamic SecYEG Translocon (2021) · cites this paper as Borowska et al., 2015

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Borowska, M. T., Dominik, P. K., Anghel, S. A., Kossiakoff, A. A., & Keenan, R. J.
Affiliations
University of Chicago (US)
Corresponding author
Keenan, Robert J.
Venue
Structure
Publisher
Elsevier BV
Published
2015-09
Volume
23
Issue
9
Pages
1715-1724
ISSN
0969-2126 (ISSN-L)
OpenAlex
W1954772973
Cited by
71
References
68
Citations by year
2026: 3 · 2025: 4 · 2024: 6 · 2023: 3 · 2022: 12 · 2021: 7 · 2020: 9 · 2019: 7 · 2018: 12 · 2017: 4 · 2016: 2 · 2015: 2
FWCI
4.56
Type
journal article
Language
en
Open access
Locations
  • Structure (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
Funders
  • NIH (P41 GM103403, U01 GM094588, U54 GM087519, R01 GM086487)
  • US DOE (DE-AC02-06CH11357)
  • Boehringer Ingelheim Fonds
  • Searle Funds at The Chicago Community Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
Keywords
YidC, membrane protein insertion, Methanococcus jannaschii, crystal structure, ribosome binding, nascent chain, archaea
MeSH terms
Cell Membrane, Models, Molecular, Phylogeny, Ribosomes, Protein Structure, Secondary, Archaeal Proteins, Membrane Transport Proteins, Methanocaldococcus

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review