A YidC-like protein in the archaeal plasma membrane.
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Cells possess specialized machinery to direct the insertion of membrane proteins into the lipid bilayer. In bacteria, the essential protein YidC inserts certain proteins into the plasma membrane, and eukaryotic orthologs are present in the mitochondrial inner membrane and the chloroplast thylakoid membrane. The existence of homologous insertases in archaea has been proposed based on phylogenetic analysis. However, limited sequence identity, distinct architecture, and the absence of experimental data have made this assignment ambiguous. Here we describe the 3.5-Å crystal structure of an archaeal DUF106 protein from Methanocaldococcus jannaschii (Mj0480), revealing a lipid-exposed hydrophilic surface presented by a conserved YidC-like fold. Functional analysis reveals selective binding of Mj0480 to ribosomes displaying a stalled YidC substrate, and a direct interaction between the buried hydrophilic surface of Mj0480 and the nascent chain. These data provide direct experimental evidence that the archaeal DUF106 proteins are YidC/Oxa1/Alb3-like insertases of the archaeal plasma membrane.
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YidC belongs to a conserved group of proteins with homologues in mitochondria, chloroplasts, the endoplasmic reticulum and archaea.
“It belongs to a conserved group of proteins with homologues in mitochondria, chloroplasts, the endoplasmic reticulum and archaea (Borowska et al., 2015; Anghel et al., 2017; Kuhn and Kiefer, 2017; McDowell et al., 2021).”
From The Dynamic SecYEG Translocon (2021) · cites this paper as Borowska et al., 2015
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- Authors
- Borowska, M. T., Dominik, P. K., Anghel, S. A., Kossiakoff, A. A., & Keenan, R. J.
- Affiliations
- University of Chicago (US)
- Corresponding author
- Keenan, Robert J.
- Venue
- Structure
- Publisher
- Elsevier BV
- Published
- 2015-09
- Volume
- 23
- Issue
- 9
- Pages
- 1715-1724
- ISSN
- 0969-2126 (ISSN-L)
- PMC
- PMC4558205
- OpenAlex
- W1954772973
- Cited by
- 71
- References
- 68
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 6 · 2023: 3 · 2022: 12 · 2021: 7 · 2020: 9 · 2019: 7 · 2018: 12 · 2017: 4 · 2016: 2 · 2015: 2
- FWCI
- 4.56
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 366 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Structure (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion)
- Funders
- NIH (P41 GM103403, U01 GM094588, U54 GM087519, R01 GM086487)
- US DOE (DE-AC02-06CH11357)
- Boehringer Ingelheim Fonds
- Searle Funds at The Chicago Community Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Keywords
- YidC, membrane protein insertion, Methanococcus jannaschii, crystal structure, ribosome binding, nascent chain, archaea
- MeSH terms
- Cell Membrane, Models, Molecular, Phylogeny, Ribosomes, Protein Structure, Secondary, Archaeal Proteins, Membrane Transport Proteins, Methanocaldococcus
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