Microbial characterization of an artisanal production of Robiola di Roccaverano cheese
Open accessAbstract
Robiola di Roccaverano, from the Piedmont region of Italy, is a Protected Designation of Origin soft cheese made with raw goat milk. The peculiarity of this cheese is that during the manufacturing process, a natural starter culture (NC) is added to raw milk. This study examined the viable microorganisms of technological interest, including lactic acid bacteria and fungal populations, in samples of raw milk, NC, and fresh and ripened cheese collected from one dairy using culture-dependent techniques. First, the isolated colonies were analyzed using random amplification of polymorphic DNA (RAPD) PCR, and strains with similar fingerprints were clustered together. Further, representative isolates of each group were subjected to 16S or 26S ribosomal DNA sequencing. Finally, species-specific PCR was conducted to distinguish the Lactococcus lactis ssp. lactis and Lc. lactis ssp. cremoris. Among the studied lactic acid bacteria, 13 RAPD profiles were obtained, corresponding to 9 different bacterial species or subspecies. Concerning mold and yeast isolates, 5 species were found that coincided with 5 RAPD types. Observing the strains isolated in the study, Lc. lactis was the most prevalent species in raw milk and NC samples, and Leuconostoc mesenteroides was the predominant species identified in 5- and 15-d cheese isolates. Furthermore, whereas only these 2 species were detected in NC, Enterococcus and Lactobacillus genera were found in raw milk and cheese, respectively. Concerning the mold and yeast isolates, in NC Kluyveromyces spp. was mainly found, and in cheese samples the representative species were Geotrichum candidum and Yarrowia lipolytica. Finally, raw milk and cheese safety were evaluated, and the samples complied with the standard required by European Commission regulation number 2073/2005.
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Within the genus Leuconostoc, the species Ln. mesenteroides, Ln. peseudomesenteroides, and Ln. citreum have been detected in artisanal cheeses produced from raw milk.
“Within the genus Leuconostoc, the species Ln. mesenteroides, Ln. peseudomesenteroides, and Ln. citreum have been detected in artisanal cheeses produced from raw milk [122,123,124,125].”
From Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions (2022) · cites this paper as [125]
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Lactic Acid Bacteria in Raw-Milk Cheeses: From Starter Cultures to Probiotic Functions
Coelho, M. C., Malcata, F. X., & Silva, C. C. G. (2022) · Foods · doi:10.3390/foods11152276 · Cited by 276 · see citing works
“Within the genus Leuconostoc , the species Ln. mesenteroides, Ln. peseudomesenteroides , and Ln. citreum have been detected in artisanal cheeses produced from raw milk [122 – 125].”
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- Authors
- Biolcati, F., Andrighetto, C., Bottero, M. T., & Dalmasso, A.
- Affiliations
- University of Turin (IT)
- Corresponding author
- Biolcati, Federica
- Venue
- Journal of Dairy Science
- Publisher
- American Dairy Science Association
- Published
- 2020-05
- Volume
- 103
- Issue
- 5
- Pages
- 4056-4067
- ISSN
- 0022-0302 (ISSN-L)
- OpenAlex
- W3011165013
- Cited by
- 26
- References
- 50
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 6 · 2023: 3 · 2022: 6 · 2021: 6 · 2020: 3
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Dairy Science (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Turin (University of Turin) (submittedVersion, other-oa)
- Funders
- Università degli Studi di Torino
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Advanced Scientific Research Methods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
- Keywords
- artisanal cheese production, raw goat milk, lactic acid bacteria, Lactococcus lactis, Leuconostoc mesenteroides, Geotrichum candidum, Yarrowia lipolytica, cheese microbiota, cheese ripening, food safety, RAPD-PCR, ribosomal DNA sequencing, species-specific PCR
- MeSH terms
- Animals, Bacteria, Cheese, Food Microbiology, Geotrichum, Goats, Kluyveromyces, Lactobacillus, Milk, Yeasts, Enterococcus, Random Amplified Polymorphic DNA Technique, Molecular Typing
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