Antibodies to Domain 4/5 (Dm4/5) of β2-Glycoprotein 1 (β2GP1) in different antiphospholipid (aPL) antibody profiles
Abstract
Background Determination of the three recommended tests for the diagnosis of antiphospholipid syndrome [Lupus Anticoagulant (LA), anticardiolipin (aCL) and anti β2-Glycoprotein 1 (aβ2GP1) antibodies] allow physicians to allocate patients into classification (risk) categories.Objectives To measure antibodies of IgG isotype directed towards Domain 4/5 (Dm4/5) of β2GP1.Patients/methods In this cross-sectional study we measured IgG aβ2GP1-Dm4/5 in a group of individuals positive for IgG aβ2GP1 and classified as triple (LAC+, IgG aCL+, IgG aβ2GP1+, n=32), double (LAC-, IgG aCL+, IgG aβ2GP1+, n=23) or single positive (LA-, IgG aCL-, IgG aβ2GP1+, n=10).Results Geometric mean and standard deviation of IgG aβ2GP1 values expressed as Chemiluminescent Units (CU) in triple, double, single positive groups and in 40 healthy individuals were 1795±783, 321±181, 29±8 and 5.0±1.0, respectively (ANOVA p<0.0001). Geometric mean and standard deviation of IgG aβ2GP1-Dm4/5 expressed as Optical Density (OD) in triple, double and single positive groups and in 40 healthy individuals were 0.16±0.13, 0.16±0.15 and 0.26±0.15, 0.13±0,11, respectively (ANOVA p<0.002). Individuals in the single positive group, expressed significantly higher values with respect to triple (p=0.04) and double (p=0.03) positive groups. Approximate OD cut-off value (99° percentile) calculated in 40 normal control subjects is 0.404. Positivity to IgG aβ2GP1-Dm4/5 according to this cutoff was found in only 5 individuals, 3 in triple positive and 2 in single positive groups and was not associated with thromboembolism.Conclusion Mean level of IgG aβ2GP1-Dm4/5 is higher in single positive group. There is no association between positivity to IgG aβ2GP1-Dm4/5 and thromboembolic events.
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These results confirm and validate several previous reports that have implicated these antibodies in the pathogenesis of APS.
“These results confirm and validate several previous reports that have implicated these antibodies in the pathogenesis of APS [9, 10, 12, 13, 29, 30], but they also add to the growing body of evidence that shows inconsistent associations between aPL-associated autoantibodies and anamnestic thrombotic risk [3, 16].”
From Cardiovascular risk factors are major determinants of thrombotic risk in patients with the lupus anticoagulant (2017) · cites this paper as [10]
Cites
MIYAKIS, S., LOCKSHIN, M. D., ATSUMI, T., BRANCH, D. W., BREY, R. L., CERVERA, R., DERKSEN, R. H. W. M., DE GROOT, P. G., KOIKE, T., MERONI, P. L., et al. (2006) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2006.01753.x · Cited by 7,203 · see citing works
Update of the guidelines for lupus anticoagulant detection
PENGO, V., TRIPODI, A., REBER, G., RAND, J. H., ORTEL, T. L., GALLI, M., & DE GROOT, P. G. (2009) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2009.03555.x · Cited by 1,197 · see citing works
Self-citationGalli, M., Luciani, D., Bertolini, G., & Barbui, T. (2003) · Blood · doi:10.1182/blood-2002-02-0441 · Cited by 974 · cited 1× · see citing works
“However, LA is still regarded as the strongest risk factor for thrombosis as demonstrated in many studies and the review of Galli et al [3].”
BackgroundClinical course of high‐risk patients diagnosed with antiphospholipid syndrome
PENGO, V., RUFFATTI, A., LEGNANI, C., GRESELE, P., BARCELLONA, D., ERBA, N., TESTA, S., MARONGIU, F., BISON, E., DENAS, G., et al. (2010) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2009.03674.x · Cited by 633 · cited 1× · see citing works
“Patients with triple laboratory profile are at high risk of both developing thrombosis or having recurrences; double and single positive patients are at lower or at no risk of having events [2].”
BackgroundSelf-citationde Laat, B., Derksen, R. H. W. M., Urbanus, R. T., & de Groot, P. G. (2005) · Blood · doi:10.1182/blood-2004-09-3387 · Cited by 378 · see citing works
DE LAAT, B., PENGO, V., PABINGER, I., MUSIAL, J., VOSKUYL, A. E., BULTINK, I. E. M., RUFFATTI, A., ROZMAN, B., KVEDER, T., DE MOERLOOSE, P., et al. (2009) · Journal of Thrombosis and Haemostasis · doi:10.1111/j.1538-7836.2009.03588.x · Cited by 291 · see citing works
Self-citationTesting for Antiphospholipid antibodies with Solid Phase Assays: guidance from the SSC of the ISTH
Devreese, K. M. J., Pierangeli, S. S., de Laat, B., Tripodi, A., Atsumi, T., & Ortel, T. L. (2014) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12537 · Cited by 224 · see citing works
Pierangeli, S., de Groot, P. G., Dlott, J. S., Favaloro, E. J., Harris, E., Lakos, G., Ortel, T. L., Meroni, P. L., Otomo, K., Pengo, V., et al. (2011) · Lupus · doi:10.1177/0961203310395055 · Cited by 179 · see citing works
Self-citationAgostinis, C., Durigutto, P., Sblattero, D., Borghi, M. O., Grossi, C., Guida, F., Bulla, R., Macor, P., Pregnolato, F., Meroni, P. L., et al. (2014) · Blood · doi:10.1182/blood-2013-11-537704 · Cited by 138 · see citing works
Pengo, V., Ruffatti, A., Tonello, M., Cuffaro, S., Banzato, A., Bison, E., Denas, G., & Padayattil Jose, S. (2015) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12865 · Cited by 126 · see citing works
Self-citationAntiphospholipid syndrome: laboratory detection, mechanisms of action and treatment
Tripodi, A., de Groot, P. G., & Pengo, V. (2011) · Journal of Internal Medicine · doi:10.1111/j.1365-2796.2011.02362.x · Cited by 113 · see citing works
Self-citationPengo, V., Ruffatti, A., Del Ross, T., Tonello, M., Cuffaro, S., Hoxha, A., Banzato, A., Bison, E., Denas, G., Bracco, A., et al. (2013) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12264 · Cited by 101 · see citing works
Self-citationAndréoli, L., Nalli, C., Motta, M., Norman, G. L., Shums, Z., Encabo, S., Binder, W. L., Nuzzo, M. D., Frassi, M., Lojacono, A., et al. (2010) · Annals of the Rheumatic Diseases · doi:10.1136/ard.2010.137281 · Cited by 92 · see citing works
Arvieux, J., Renaudineau, Y., Mané, I., et al. (2002) · PubMed · Cited by 77 · see citing works
Biasiolo, A., Rampazzo, P., Brocco, T., & Pengo, V. (1999) · Thrombosis and Haemostasis · doi:10.1055/s-0037-1614453 · Cited by 76 · see citing works
Self-citationAnti-beta2-glycoprotein I antibodies in children with atopic dermatitis
Ambrožič, A. (2002) · International Immunology · doi:10.1093/intimm/dxf043 · Cited by 74 · see citing works
Banzato, A., Pozzi, N., Frasson, R., Filippis, V. D., Ruffatti, A., Bison, E., Jose, S. P., Denas, G., & Pengo, V. (2011) · Thrombosis Research · doi:10.1016/j.thromres.2011.04.021 · Cited by 73 · see citing works
Self-citationIverson, G. M., von Mühlen, C. A., Staub, H. L., Lassen, A. J., Binder, W. L., & Norman, G. L. (2006) · Journal of Autoimmunity · doi:10.1016/j.jaut.2006.09.007 · Cited by 73 · see citing works
Renaudineau, Y., Mane, I., Perraut, R., et al. (2002) · Thrombosis and Haemostasis · doi:10.1055/s-0037-1613055 · Cited by 64 · see citing works
Rosendaal, F. R., & Reitsma, P. H. (2012) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.12069 · Cited by 28 · see citing works
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Laboratory criteria for antiphospholipid syndrome: communication from the SSC of the ISTH
Devreese, K. M. J., Ortel, T. L., Pengo, V., & de Laat, B. (2018) · Journal of Thrombosis and Haemostasis · doi:10.1111/jth.13976 · Cited by 269 · see citing works
Self-citationPosch, F., Gebhart, J., Rand, J. H., Koder, S., Quehenberger, P., Pengo, V., Ay, C., & Pabinger, I. (2017) · BMC Medicine · doi:10.1186/s12916-017-0807-7 · Cited by 25 · see citing works
“Miyakis S, Lockshin MD, Atsumi T, Branch DW, Brey RL, Cervera R, Derksen RH, de Groot PG, Koike T, Meroni PL, et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS).”
ResultInfluentialSelf-citation
- Authors
- Pengo, V., Ruffatti, A., Tonello, M., Hoxha, A., Bison, E., Denas, G., Padayattil Jose, S., Zoppellaro, G., Bracco, A., & Banzato, A.
- Affiliations
- University of Padua (IT)
- Corresponding author
- Pengo, V.
- Venue
- Thrombosis Research
- Publisher
- Elsevier BV
- Published
- 2015-07
- Volume
- 136
- Issue
- 1
- Pages
- 161-163
- ISSN
- 0049-3848 (ISSN-L)
- OpenAlex
- W2038851808
- Cited by
- 47
- References
- 20
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 2 · 2022: 2 · 2020: 4 · 2019: 5 · 2018: 10 · 2017: 7 · 2016: 8 · 2015: 2
- FWCI
- 7.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Thrombosis Research (publishedVersion)
- PubMed (publishedVersion)
- Padua Research Archive (University of Padova) (submittedVersion)
- Topics
- Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Vasculitis and related conditions
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Keywords
- beta2-glycoprotein I, antiphospholipid antibodies, antiphospholipid syndrome, lupus anticoagulant, anticardiolipin antibodies, IgG, antibody profiles, cut-off values, serological diagnosis, autoantibodies, thrombosis risk stratification, chemiluminescence immunoassay
- MeSH terms
- Epitopes, Cross-Sectional Studies, Humans, Immunoglobulin G, Antiphospholipid Syndrome, Antibodies, Antiphospholipid, Protein Structure, Tertiary, beta 2-Glycoprotein I
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