Cited by 181Effects and risks of endocrine disrupting chemicalsEpigenetics and DNA MethylationToxic Organic Pollutants Impact

Effects of endocrine disrupting chemicals on in vitro global DNA methylation and adipocyte differentiation

Bastos Sales, L., Kamstra, J.H., Cenijn, P.H., van Rijt, L.S., Hamers, T., Legler, J.·2013·Toxicology in Vitro

Abstract

Recent studies suggest that endocrine disrupting chemicals (EDCs) may form a risk factor for obesity by altering energy metabolism through epigenetic gene regulation. The goal of this study is to investigate the effects of a range of EDCs with putative obesogenic properties on global DNA methylation and adipocyte differentiation in vitro. Murine N2A and human SK-N-AS neuroblastoma cells and murine preadipocyte fibroblasts (3T3-L1) were exposed to tributyltin (TBT), diethylstilbestrol (DES), bisphenol A (BPA), 2,3,7,8-tetrachlorodibenzo-[p]-dioxin (TCDD), 2,2',4,4',5,5'-hexachlorobiphenyl (PCB-153), hexachlorobenzene (HCB), hexabromocyclododecane (HBCD), 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) , perfluorinated octyl acid (PFOA) and perfluorinated octyl sulfonate (PFOS). A modest decrease in global DNA methylation was observed in N2A cells exposed to 10 μM DES, BPA, TCDD, BDE-47, PCB-153 and 1 μM HCB, but no changes were found in the human SK-N-AS cells. We reveal for the first time that BDE-47 increases adipocyte differentiation in a dose-dependent manner (2.5-25 μM). Adipocyte differentiation was also enhanced by TBT (≥ 10 nM) and BPA (>10 μM) and inhibited by TCDD (≥ 0.1 nM). The other chemicals showed either modest or no effects on adipocyte differentiation at the concentrations tested (PFOA, PFOS and HBCD at 10 μM; PCB-153, 3.4 μM and HCB, 1 μM). This study demonstrates that selected EDCs can induce functional changes in murine adipocyte differentiation in vitro which are accompanied by decreased global DNA methylation.

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Crossref · 10.1016/j.tiv.2013.04.005

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Authors
Bastos Sales, L., Kamstra, J. H., Cenijn, P. H., van Rijt, L. S., Hamers, T., & Legler, J.
Affiliations
Vrije Universiteit Amsterdam (NL)
Corresponding author
Legler, J.
Venue
Toxicology in Vitro
Publisher
Elsevier BV
Published
2013-09
Volume
27
Issue
6
Pages
1634-1643
ISSN
0887-2333 (ISSN-L)
OpenAlex
W2015008373
Cited by
181
References
48
Citations by year
2026: 4 · 2025: 4 · 2024: 27 · 2023: 7 · 2022: 13 · 2021: 14 · 2020: 14 · 2019: 10 · 2018: 21 · 2017: 18 · 2016: 15 · 2015: 10 · 2014: 23 · 2013: 1
FWCI
11.98
Type
journal article
Language
en
Open access
closed
Locations
  • Toxicology in Vitro (publishedVersion)
  • PubMed (publishedVersion)
  • Pure Amsterdam UMC (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • VU Research Portal (submittedVersion)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
Keywords
endocrine disruptors, global DNA methylation, adipogenesis, obesity, epigenetic regulation, bisphenol A, diethylstilbestrol, tributyltin, 2,3,7,8-tetrachlorodibenzo-p-dioxin, BDE-47, hexabromocyclododecane, PCB 153, hexachlorobenzene
MeSH terms
Bisphenol A Compounds, Animals, Benzhydryl Compounds, Caprylates, Cell Differentiation, Diethylstilbestrol, Environmental Pollutants, Fluorocarbons, Humans, Hydrocarbons, Brominated, Hydrocarbons, Chlorinated, Phenols, Trialkyltin Compounds, Adipocytes, Alkanesulfonic Acids, DNA Methylation, 3T3-L1 Cells, Cell Line, Tumor, Mice, Endocrine Disruptors, Halogenated Diphenyl Ethers

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