High calorie diet background alters the expression of sirtuins in the testes of mice under caloric restriction
Open accessAbstract
Caloric restriction (CR) is considered one of the most efficient interventions that extend lifespan and healthspan by delaying the onset of many age-related diseases in a variety of organisms. CR appears to activate sirtuins, which in turn modulate a wide range of cellular activities related to pathophysiological conditions such as stress and inflammation, DNA repair, apoptosis, and the processes of aging. In this work we aimed to determine whether CR can counteract the detrimental effects of a high-calorie diet (HCD) on the mRNA and protein levels of sirtuins in testes of a murine experimental model. We report in here that short-term CR increased the mRNA and protein expression of all sirtuins in the animals previously feed standard food. However, short-term CR failed to activate sirtuins in the animals previously fed an HCD for a period of 4 months. Moreover, histologic analysis of adipocyte/lipid infiltration by oil red O assay, in the mice testis, showed that the highest lipid content was found in the animals with an HCD background, exposed to CR. Mechanistic actions, as well as substrates that modify sirtuins transcriptional and translational expression in the mice male reproductive system, are still not well understood. We believe that deeper insights into how the potential selective modulation of SIRT family members and sirtuins activators affect the different organs, individually and as a whole system will eventually led to the development of preventative and therapeutic strategies to promote health and longevity.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Short-term caloric restriction in mice increases expression of all seven sirtuin (SIRT1-SIRT7) proteins.
“Short-term caloric restriction in mice increases expression of all seven sirtuin (SIRT1-SIRT7) proteins (Maldonado et al. 2021) and overexpression of telomerase reverse transcriptase (TERT) which reduces telomere erosion from various tissues such as muscles, kidney, and lungs (Vera et al. 2013).”
From Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging (2023) · cites this paper as Maldonado et al. 2021
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Paradoxical Decrease of an Adipose-Specific Protein, Adiponectin, in Obesity
Arita, Y., Kihara, S., Ouchi, N., Takahashi, M., Maeda, K., Miyagawa, J.-I., Hotta, K., Shimomura, I., Nakamura, T., Miyaoka, K., et al. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0255 · Cited by 4,856 · see citing works
Oxidative Stress, Caloric Restriction, and Aging
Sohal, R. S., & Weindruch, R. H. (1996) · Science · doi:10.1126/science.273.5271.59 · Cited by 3,133 · see citing works
Extending Healthy Life Span—From Yeast to Humans
Fontana, L., Partridge, L., & Longo, V. D. (2010) · Science · doi:10.1126/science.1172539 · Cited by 3,023 · see citing works
Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase
Cohen, H. Y., Miller, C., Bitterman, K. J., Wall, N. R., Hekking, B., Kessler, B., Howitz, K. T., Gorospe, M., de Cabo, R., & Sinclair, D. A. (2004) · Science · doi:10.1126/science.1099196 · Cited by 1,924 · see citing works
Sirtuin activators mimic caloric restriction and delay ageing in metazoans
Wood, J. G., Rogina, B., Lavu, S., Howitz, K., Helfand, S. L., Tatar, M., & Sinclair, D. (2004) · Nature · doi:10.1038/nature02789 · Cited by 1,868 · see citing works
Recent progress in the biology and physiology of sirtuins
Finkel, T., Deng, C., & Mostoslavsky, R. (2009) · Nature · doi:10.1038/nature08197 · Cited by 1,484 · see citing works
Michishita, E., Park, J. Y., Burneskis, J. M., Barrett, J. C., & Horikawa, I. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-01-0033 · Cited by 1,364 · see citing works
Genetic pathways that regulate ageing in model organisms
Guarente, L., & Kenyon, C. J. (2000) · Nature · doi:10.1038/35041700 · Cited by 1,337 · see citing works
Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation
Lombard, D. B., Alt, F. W., Cheng, H.-L., Bunkenborg, J., Streeper, R. S., Mostoslavsky, R., Kim, J., Yancopoulos, G., Valenzuela, D., Murphy, A., et al. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.01636-07 · Cited by 1,216 · see citing works
Haigis, M. C., Mostoslavsky, R., Haigis, K. M., Fahie, K., Christodoulou, D. C., Murphy, A. J., Valenzuela, D. M., Yancopoulos, G. D., Karow, M., Blander, G., et al. (2006) · Cell · doi:10.1016/j.cell.2006.06.057 · Cited by 1,162 · see citing works
SIRT1 and other sirtuins in metabolism
Chang, H.-C., & Guarente, L. (2014) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2013.12.001 · Cited by 1,138 · see citing works
Metabolic regulation of gene expression through histone acylations
Sabari, B. R., Zhang, D., Allis, C. D., & Zhao, Y. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.140 · Cited by 1,134 · see citing works
SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin
Michishita, E., McCord, R. A., Berber, E., Kioi, M., Padilla-Nash, H., Damian, M., Cheung, P., Kusumoto, R., Kawahara, T. L. A., Barrett, J. C., et al. (2008) · Nature · doi:10.1038/nature06736 · Cited by 1,111 · see citing works
The sirtuin SIRT6 regulates lifespan in male mice
Kanfi, Y., Naiman, S., Amir, G., Peshti, V., Zinman, G., Nahum, L., Bar-Joseph, Z., & Cohen, H. Y. (2012) · Nature · doi:10.1038/nature10815 · Cited by 1,059 · see citing works
Overview of caloric restriction and ageing
Masoro, E. J. (2005) · Mechanisms of Ageing and Development · doi:10.1016/j.mad.2005.03.012 · Cited by 1,054 · see citing works
The Histone Deacetylase Sirt6 Regulates Glucose Homeostasis via Hif1α
Zhong, L., D'Urso, A., Toiber, D., Sebastian, C., Henry, R. E., Vadysirisack, D. D., Guimaraes, A., Marinelli, B., Wikstrom, J. D., Nir, T., et al. (2010) · Cell · doi:10.1016/j.cell.2009.12.041 · Cited by 1,001 · see citing works
Adiponectin and AdipoR1 Regulate PGC-1α and Mitochondria by Ca2+ and AMPK/SIRT1
Iwabu, M., Yamauchi, T., Okada-Iwabu, M., Sato, K., Nakagawa, T., Funata, M., Yamaguchi, M., Namiki, S., Nakayama, R., Tabata, M., et al. (2010) · Nature · doi:10.1038/nature08991 · Cited by 971 · see citing works
SIRT5 deacetylates carbamoyl phosphate synthetase 1 and regulates the urea cycle
Nakagawa, T., Lomb, D. J., Haigis, M. C., & Guarente, L. (2009) · Cell · doi:10.1016/j.cell.2009.02.026 · Cited by 787 · see citing works
Kim, H.-S., Patel, K., Muldoon-Jacobs, K., Bisht, K. S., Aykin-Burns, N., Pennington, J. D., van der Meer, R., Nguyen, P., Savage, J., Owens, K. M., et al. (2010) · Cancer Cell · doi:10.1016/j.ccr.2009.11.023 · Cited by 785 · see citing works
Mammalian Sir2 homolog SIRT7 is an activator of RNA polymerase I transcription
Ford, E., Voit, R., Liszt, G., Magin, C., Grummt, I., & Guarente, L. (2006) · Genes & Development · doi:10.1101/gad.1399706 · Cited by 629 · see citing works
The Mammalian SIR2α Protein Has a Role in Embryogenesis and Gametogenesis
McBurney, M. W., Yang, X., Jardine, K., Hixon, M., Boekelheide, K., Webb, J. R., Lansdorp, P. M., & Lemieux, M. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.1.38-54.2003 · Cited by 611 · see citing works
SIRT2 Regulates Adipocyte Differentiation through FoxO1 Acetylation/Deacetylation
Jing, E., Gesta, S., & Kahn, C. R. (2007) · Cell Metabolism · doi:10.1016/j.cmet.2007.07.003 · Cited by 490 · see citing works
A mitochondrial UPR-mediated metabolic checkpoint regulates hematopoietic stem cell aging
Mohrin, M., Shin, J., Liu, Y., Brown, K., Luo, H., Xi, Y., Haynes, C. M., & Chen, D. (2015) · Science · doi:10.1126/science.aaa2361 · Cited by 490 · see citing works
Protective effects of sirtuins in cardiovascular diseases: from bench to bedside
Winnik, S. H., Auwerx, J. H., Sinclair, D. A., & Matter, C. M. (2015) · European Heart Journal · doi:10.1093/eurheartj/ehv290 · Cited by 443 · see citing works
Regulation of Insulin Secretion by SIRT4, a Mitochondrial ADP-ribosyltransferase
Ahuja, N., Schwer, B., Carobbio, S., Waltregny, D., North, B. J., Castronovo, V., Maechler, P., & Verdin, E. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m705488200 · Cited by 422 · see citing works
Adiponectin Receptor as a Key Player in Healthy Longevity and Obesity-Related Diseases
Yamauchi, T., & Kadowaki, T. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.01.001 · Cited by 415 · see citing works
Krishnan, J., Danzer, C. F., Simka, T., Ukropec, J., Walter, K., Kumpf, S., Mirtschink, P., Ukropcová, B., Gašperíková, D., Pedrazzini, T., et al. (2012) · Genes & Development · doi:10.1101/gad.180406.111 · Cited by 316 · see citing works
SIRT6 protects against pathological damage caused by diet‐induced obesity
Kanfi, Y., Peshti, V., Gil, R., Naiman, S., Nahum, L., Levin, E., Kronfeld‐Schor, N., & Cohen, H. Y. (2010) · Aging Cell · doi:10.1111/j.1474-9726.2009.00544.x · Cited by 311 · see citing works
A SIRT7-Dependent Acetylation Switch of GABPβ1 Controls Mitochondrial Function
Ryu, D., Jo, Y. S., Sasso, G. L., Stein, S., Zhang, H., Perino, A., Lee, J. U., Zeviani, M., Romand, R., Hottiger, M. O., et al. (2014) · Cell Metabolism · doi:10.1016/j.cmet.2014.08.001 · Cited by 258 · see citing works
SIRT7 Controls Hepatic Lipid Metabolism by Regulating the Ubiquitin-Proteasome Pathway
Yoshizawa, T., Karim, M. F., Sato, Y., Senokuchi, T., Miyata, K., Fukuda, T., Go, C., Tasaki, M., Uchimura, K., Kadomatsu, T., et al. (2014) · Cell Metabolism · doi:10.1016/j.cmet.2014.03.006 · Cited by 217 · see citing works
Wang, F., Tian, X., Zhang, L., He, C., Ji, P., Li, Y., Tan, D. X., & Liu, G. (2013) · Fertility and Sterility · doi:10.1016/j.fertnstert.2013.10.041 · Cited by 201 · see citing works
Protein post-translational modifications: In silico prediction tools and molecular modeling
Audagnotto, M., & Peraro, M. D. (2017) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2017.03.004 · Cited by 189 · see citing works
Sirtuins and Disease: The Road Ahead
Carafa, V., Nebbioso, A., & Altucci, L. (2012) · Frontiers in Pharmacology · doi:10.3389/fphar.2012.00004 · Cited by 179 · see citing works
Rato, L., Alves, M. G., Dias, T. R., Lopes, G., Cavaco, J. E. B., Socorro, S., & Oliveira, P. F. (2013) · Andrology · doi:10.1111/j.2047-2927.2013.00071.x · Cited by 174 · see citing works
Rato, L., Duarte, A. I., Tomás, G. D., Santos, M. S., Moreira, P. I., Socorro, S., Cavaco, J. E. B., Alves, M. G., & Oliveira, P. F. (2013) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2013.12.008 · Cited by 154 · see citing works
Sirt1 Deficiency Attenuates Spermatogenesis and Germ Cell Function
Coussens, M. J., Maresh, J. G., Yanagimachi, R., Maeda, G., & Allsopp, R. (2008) · PLoS ONE · doi:10.1371/journal.pone.0001571 · Cited by 153 · see citing works
Handbook of the Biology of Aging
(2016) · Elsevier eBooks · doi:10.1016/c2012-0-07319-x · Cited by 136 · see citing works
Kelly, G. S. (2010) · PubMed · Cited by 135 · see citing works
Sirtuin 7 Plays a Role in Ribosome Biogenesis and Protein Synthesis
Tsai, Y.-C., Greco, T. M., & Cristea, I. M. (2013) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m113.031377 · Cited by 124 · see citing works
SirT1 is required in the male germ cell for differentiation and fecundity in mice
Bell, E. L., Nagamori, I., Williams, E. O., Rosario, A. M. D., Bryson, B. D., Watson, N., White, F. M., Sassone‐Corsi, P., & Guarente, L. (2014) · Development · doi:10.1242/dev.110627 · Cited by 116 · see citing works
SIRT6 in mouse spermatogenesis is modulated by diet-induced obesity
McPherson, N. O., Fullston, T., Mitchell, M., Setchell, B. P., & Lane, M. (2011) · Reproduction Fertility and Development · doi:10.1071/rd10326 · Cited by 101 · see citing works
Vakhrusheva, O., Braeuer, D., Liu, Z., Braun, T. W., & Bober, E. (2008) · PubMed · Cited by 85 · see citing works
SIRT4 is the last puzzle of mitochondrial sirtuins
Li, Y., Zhou, Y., Wang, F., Chen, X., Wang, C., Wang, J., Liu, T., Li, Y., & He, B. (2018) · Bioorganic & Medicinal Chemistry · doi:10.1016/j.bmc.2018.07.031 · Cited by 63 · see citing works
Kelly, G. S. (2010) · PubMed · Cited by 63 · see citing works
In Search of New Therapeutic Targets in Obesity Treatment: Sirtuins
Kuryłowicz, A. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17040572 · Cited by 43 · see citing works
Sirtuins: Novel Players in Male Reproductive Health
Rato, L., Alves, M. G., Silva, B. M., Sousa, M., & Oliveira, P. F. (2016) · Current Medicinal Chemistry · doi:10.2174/0929867323666160229114248 · Cited by 35 · see citing works
Caloric restriction: life span extension and retardation of brain aging
Weindruch, R. H. (2003) · Clinical Neuroscience Research · doi:10.1016/s1566-2772(03)00004-5 · Cited by 24 · see citing works
Loss of mitochondrial SIRT4 shortens lifespan and leads to a decline in physical activity
Parik, S., Tewary, S., Ayyub, C., & Kolthur‐Seetharam, U. (2018) · Journal of Biosciences · doi:10.1007/s12038-018-9754-5 · Cited by 13 · see citing works
Sirtuins, Healthspan, and Longevity in Mammals
Giblin, W., & Lombard, D. B. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-411596-5.00003-4 · Cited by 12 · see citing works
Flagged first, then by citations
5 of the 54 references couldn’t be matched to a known paper.
Research works printed but not linked: 2
- M.W. McBurney, et al., The mammalian SIR2a protein has a role in embryo- genesis and gametogenesis, Mol. Cell Biol. (2003), https://doi.org/10.1128/ mcb.23.1.38-54.2003. · DOI · Paper page
- Y. Kanfi, et al., The sirtuin SIRT6 regulates lifespan in male mice, Nature (2012), https://doi.org/10.1038/nature10815. · DOI · Paper page
Cited by
Sorted by citations
Reactive Oxygen Species, Toxicity, Oxidative Stress, and Antioxidants: Chronic Diseases and Aging
Jomova, K., Raptova, R., Alomar, S. Y., Alwasel, S. H., Nepovimova, E., Kuca, K., & Valko, M. (2023) · Archives of Toxicology · doi:10.1007/s00204-023-03562-9 · Cited by 2,638 · see citing works
Why Is Longevity Still a Scientific Mystery? Sirtuins—Past, Present and Future
Ziętara, P., Dziewięcka, M., & Augustyniak, M. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms24010728 · Cited by 40 · see citing works
A nutrigeroscience approach: Dietary macronutrients and cellular senescence
Calubag, M. F., Robbins, P. D., & Lamming, D. W. (2024) · Cell Metabolism · doi:10.1016/j.cmet.2024.07.025 · Cited by 32 · see citing works
Modulation of Sirtuins to address aging related disorders through the use of selected phytochemicals
Wang, J., Ren, Y., & Qu, S. (2025) · Phytomedicine · doi:10.1016/j.phymed.2025.156648 · Cited by 15 · see citing works
Matoba, H., Fujii, C., Maruyama, K., Kawakubo, M., Momose, M., Sano, K., Imamura, H., Kurihara, H., & Nakayama, J. (2024) · Endocrinology · doi:10.1210/endocr/bqae017 · Cited by 12 · see citing works
Rachakatla, A., Srinivas, M., Satyavani, M., Suresh, K., Muralidhar, M. N., & Kalashikam, R. R. (2024) · Lipids in Health and Disease · doi:10.1186/s12944-024-02161-6 · Cited by 11 · see citing works
Rachakatla, A., Myadara, S., Motha, S., Kondeti, S., Muralidhar, M. N., & Rao, K. R. (2024) · Research Square · doi:10.21203/rs.3.rs-4154129/v1
Rachakatla, A., Srinivas, M., Satyavani, M., Suresh, K., Muralidhar, M., & Rajender Rao, K. (2024) · Figshare · doi:10.6084/m9.figshare.26731366
Rachakatla, A., Srinivas, M., Satyavani, M., Suresh, K., Muralidhar, M., & Rajender Rao, K. (2024) · Figshare · doi:10.6084/m9.figshare.25998637
Rachakatla, A., Srinivas, M., Satyavani, M., Suresh, K., Muralidhar, M., & Rajender Rao, K. (2024) · Figshare · doi:10.6084/m9.figshare.25998610
May, M., Fabela, R. F., & Tomanek, L. (2026) · Frontiers in Ecology and Evolution · doi:10.3389/fevo.2026.1914093
- Authors
- Maldonado, M., Chen, J., Duan, H., Huang, T., Jiang, G., & Zhong, Y.
- Corresponding author
- Zhong, Ying
- Venue
- Translational Medicine of Aging
- Publisher
- Elsevier BV
- Published
- 2021
- Volume
- 5
- Pages
- 10-16
- ISSN
- 2468-5011 (ISSN-L)
- OpenAlex
- W3129618398
- Cited by
- 11
- References
- 54
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 7 · 2023: 1 · 2022: 1
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Translational Medicine of Aging (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Keywords
- caloric restriction, hypercaloric diet, sirtuins, testis, male genitalia, aging, fatty infiltration, adipocytes, longevity
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
