Mammalian Sir2 homolog SIRT7 is an activator of RNA polymerase I transcription
Open accessAbstract
We investigated the role of SIRT7, one of the seven members of the mammalian sirtuin family. We show that SIRT7 is a widely expressed nucleolar protein that is associated with active rRNA genes (rDNA), where it interacts with RNA polymerase I (Pol I) as well as with histones. Overexpression of SIRT7 increases Pol I-mediated transcription, whereas knockdown of SIRT7 or inhibition of the catalytic activity results in decreased association of Pol I with rDNA and a reduction of Pol I transcription. Depletion of SIRT7 stops cell proliferation and triggers apoptosis. Our findings suggest that SIRT7 is a positive regulator of Pol I transcription and is required for cell viability in mammals.
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SIRT7 appears necessary for preserving active rDNA transcription under conditions of cellular growth and stress.
“In contrast to the broadly repressive actions often attributed to other sirtuins, SIRT7 appears necessary for preserving active rDNA transcription under conditions of cellular growth and stress [183,184].”
From Ribosome Biogenesis as a Putative Bottleneck to Skeletal Muscle Hypertrophy: Mechanisms, Human Evidence, and Practical Modulators (2026) · cites this paper as [183]
In “SIRT7 activates Pol I transcription” (page not provided), SIRT7 knockdown reduces pre-rRNA synthesis and Pol I association with rDNA; the paper also links SIRT7 activity to cell growth. It does not show that SIRT7 preserves active rDNA transcription under stress conditions. Its discussion of starvation and DNA damage describes effects on pre-rRNA synthesis generally, not a demonstrated protective role for SIRT7. (LLM, conf=0.85)
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“Among them, SIRT7 has attracted interest because of its prominent nucleolar localization and its involvement in the regulation of Pol I-dependent transcription [183].”
- Authors
- Ford, E., Voit, R., Liszt, G., Magin, C., Grummt, I., & Guarente, L.
- Affiliations
- Massachusetts Institute of Technology (US)
- German Cancer Research Center (DE)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2006-04-17
- Volume
- 20
- Issue
- 9
- Pages
- 1075-1080
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2171723578
- Cited by
- 629
- References
- 28
- Citations by year
- 2026: 23 · 2025: 22 · 2024: 20 · 2023: 34 · 2022: 25 · 2021: 50 · 2020: 33 · 2019: 28 · 2018: 43 · 2017: 35 · 2016: 37 · 2015: 41 · 2014: 28 · 2013: 54 · 2012: 36
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- National Institutes of Health
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- SIRT7, sirtuins, RNA polymerase I, rDNA transcription, nucleolus, histone deacetylation, cell proliferation, apoptosis, cell viability
- MeSH terms
- Animals, Cell Line, Cell Survival, DNA, Ribosomal, Histones, Humans, RNA Polymerase I, Transcriptional Activation, Apoptosis, Genes, rRNA, RNA Interference, Sirtuins, Cell Proliferation, Mice
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