Leveraging the immunoinformatics approach for designing the SARS-CoV-2 omicron-specific antigenic cassette of mRNA vaccine
Abstract
Emergence of SARS-CoV-2 Omicron variant has presented a significant challenge to global health, demanding rapid development of mRNA-based vaccines. The mRNA-guided vaccine platforms offer various advantages over traditional vaccine platforms. The mRNA by nature is a short-lived molecule that guides the cells to manufacture antigenic proteins. In the present work, we have created an omicron spike antigenic protein sequence characterized by base composition analysis, modeling, and docking with the ACE-2 receptor. Further, we predicted the B-cell and T-cell epitopes followed by antigenicity, toxicity, and allergenicity. Finally, the protein was reverse translated, codon-optimized, and encoding mRNA sequence was checked for its stability by predicting the secondary structures. A comprehensive examination of in-silico data revealed 628.2 as a potent antigenic candidate that was finally used in Gemcovac®-OM, a heterologous booster mRNA vaccine for COVID-19.
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GEMCOVAC-OM is a monovalent, Omicron-specific (BA.1) booster mRNA vaccine for COVID-19.
“we developed GEMCOVAC-OM, a monovalent, Omicron-specific (BA.1) booster mRNA vaccine for COVID-19 (ref. 5).”
From An Omicron-specific, self-amplifying mRNA booster vaccine for COVID-19: a phase 2/3 randomized trial (2024) · cites this paper as [5]
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An Omicron-specific, self-amplifying mRNA booster vaccine for COVID-19: a phase 2/3 randomized trial
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- Authors
- Wadapurkar, R., Singh, S., & Singh, A.
- Corresponding author
- Singh, Sanjay
- Singh, Ajay
- Venue
- Vaccine
- Publisher
- Elsevier BV
- Published
- 2024-03
- Volume
- 42
- Issue
- 7
- Pages
- 1630-1647
- ISSN
- 0264-410X (ISSN-L)
- OpenAlex
- W4391686203
- Cited by
- 6
- References
- 55
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Vaccine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Government of India Department of Biotechnology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- Omicron variant, mRNA vaccines, spike protein antigen, immunoinformatics, B-cell epitopes, T cell epitopes, antigenicity prediction, allergenicity prediction, codon optimization, mRNA secondary structure, COVID-19 vaccine design
- MeSH terms
- COVID-19, SARS-CoV-2, COVID-19 Vaccines, mRNA Vaccines, Immunoinformatics, Antigens, Viral, Humans, RNA, Messenger, Epitopes, T-Lymphocyte, Epitopes, B-Lymphocyte, Molecular Docking Simulation
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