Cited by 6vaccines and immunoinformatics approachesSARS-CoV-2 and COVID-19 ResearchImmunotherapy and Immune Responses

Leveraging the immunoinformatics approach for designing the SARS-CoV-2 omicron-specific antigenic cassette of mRNA vaccine

Wadapurkar, Rucha, Singh, Sanjay, Singh, Ajay·2024·Vaccine

Abstract

Emergence of SARS-CoV-2 Omicron variant has presented a significant challenge to global health, demanding rapid development of mRNA-based vaccines. The mRNA-guided vaccine platforms offer various advantages over traditional vaccine platforms. The mRNA by nature is a short-lived molecule that guides the cells to manufacture antigenic proteins. In the present work, we have created an omicron spike antigenic protein sequence characterized by base composition analysis, modeling, and docking with the ACE-2 receptor. Further, we predicted the B-cell and T-cell epitopes followed by antigenicity, toxicity, and allergenicity. Finally, the protein was reverse translated, codon-optimized, and encoding mRNA sequence was checked for its stability by predicting the secondary structures. A comprehensive examination of in-silico data revealed 628.2 as a potent antigenic candidate that was finally used in Gemcovac®-OM, a heterologous booster mRNA vaccine for COVID-19.

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Crossref · 10.1016/j.vaccine.2024.01.087

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Authors
Wadapurkar, R., Singh, S., & Singh, A.
Corresponding author
  • Singh, Sanjay
  • Singh, Ajay
Venue
Vaccine
Publisher
Elsevier BV
Published
2024-03
Volume
42
Issue
7
Pages
1630-1647
ISSN
0264-410X (ISSN-L)
OpenAlex
W4391686203
Cited by
6
References
55
Citations by year
2026: 1 · 2025: 2 · 2024: 3
FWCI
0.81
Type
journal article
Language
en
Open access
closed
Locations
  • Vaccine (publishedVersion)
  • PubMed (publishedVersion)
Funders
Government of India Department of Biotechnology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
Keywords
Omicron variant, mRNA vaccines, spike protein antigen, immunoinformatics, B-cell epitopes, T cell epitopes, antigenicity prediction, allergenicity prediction, codon optimization, mRNA secondary structure, COVID-19 vaccine design
MeSH terms
COVID-19, SARS-CoV-2, COVID-19 Vaccines, mRNA Vaccines, Immunoinformatics, Antigens, Viral, Humans, RNA, Messenger, Epitopes, T-Lymphocyte, Epitopes, B-Lymphocyte, Molecular Docking Simulation

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