Cutaneous distribution and localization of Cercopithifilaria sp. microfilariae in dogs
Abstract
The aim of this study was to investigate the anatomical distribution of microfilariae of a recently described Cercopithifilaria sp. and the relationship with the preferred attachment sites of its vector, the brown dog tick Rhipicephalus sanguineus. Skin samples from 20 dogs were collected from eight anatomical sites and soaked twice in saline solution at 37°C. All samples were also molecularly processed for the specific amplification of partial cytochrome c oxidase 1 gene. Microfilariae were unevenly distributed on the body, with higher frequencies on interscapular region (n=13; 68.4%) and on the head (n=9; 47.4%). Larval abundance in dogs was positively correlated with the number of positive anatomical sites with a higher percentage of skin samples scoring positive at the microscopic examination of the first sediment (n=47; 30.9%) than of the second (n=8; 5.3%). Histological examination revealed that microfilariae were scattered in the dermis, in association with inflammatory cells. Molecular analysis of skin samples showed a lower frequency of positive sites (n=37; 24.3%) when compared to the microscopic examination (n=53; 34.9%) with five haplotypes sequenced. The results here presented suggested a close co-evolution of this filarioid with its vector.
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Microfilariae of Cercopithifilaria spp. are unevenly distributed on the body of an infected dog, with higher frequencies on the interscapular region and on the head, where tick vectors usually attach.
“Interestingly, it has been demonstrated that microfilariae of the latter species are unevenly distributed on the body of an infected dog with, however, higher frequencies on the interscapular region and on the head, where the tick vectors usually attach [38].”
From Vector-borne helminths of dogs and humans in Europe (2013) · cites this paper as [38]
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- Authors
- Otranto, D., Brianti, E., Abramo, F., Gaglio, G., Napoli, E., Latrofa, M. S., Ramos, R. A., Dantas-Torres, F., & Bain, O.
- Affiliations
- University of Bari Aldo Moro (IT)
- University of Messina (IT)
- University of Pisa (IT)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Otranto, Domenico
- ORCID
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2012-11
- Volume
- 190
- Issue
- 1-2
- Pages
- 143-150
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2030343607
- Cited by
- 35
- References
- 25
- Citations by year
- 2025: 1 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 8 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 2 · 2014: 6 · 2013: 5
- FWCI
- 2.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Arca - Repositório Institucional da Fiocruz (submittedVersion)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Keywords
- microfilariae, dogs, Rhipicephalus sanguineus, anatomical localization, cytochrome c oxidase 1, haplotypes, tick-borne parasites, coevolution, dermis, skin histology
- MeSH terms
- Animals, Arachnid Vectors, Base Sequence, Electron Transport Complex IV, Dog Diseases, Dogs, Female, Filariasis, Filarioidea, Haplotypes, Larva, Male, Microfilariae, Molecular Sequence Data, Phylogeny, Skin, Sequence Analysis, DNA, Rhipicephalus sanguineus
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