Rhipicephalus sanguineus (Ixodida, Ixodidae) as intermediate host of a canine neglected filarial species with dermal microfilariae
Abstract
The life cycles of filarioids of dogs presenting dermal microfilariae have been little studied. Following the recent retrieval of dermal microfilariae identified as Cercopithifilaria sp. in a dog from Sicily (Italy), this study was designed to assess the role of the brown dog tick Rhipicephalus sanguineus as an intermediate host of this filarial species. An experimental tick infestation was performed on an infected dog using 300 nymphs of R. sanguineus. Engorged nymphs were collected and examined by both microscopic dissection and molecular analysis at five time points (i.e., the same day of tick detachment and 10, 20, 30 and 50 days post-detachment) to detect the presence and developmental stage of filariae in the ticks. A total of 270 engorged nymphs were collected from the dog and developing filarioid larvae detected in 10 (5%) out of 200 ticks dissected. Infective third-stage larvae were observed in 4 (2%) of the all dissected ticks, 30 days post-detachment. Twelve (6.6%) out of 181 samples molecularly tested were positive for Cercopithifilaria sp. This study demonstrates that nymphs of R. sanguineus feeding on a dog naturally infected by Cercopithifilaria sp. can ingest microfilariae, which develop up to the third infective stage thus suggesting that this tick species might act as an intermediate host of this little known canine filarioid.
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For Cercopithifilaria sp.1, Rhipicephalus sanguineus is a competent intermediate host.
“for Cercopithifilaria sp.1, the competence of the brown dog tick, Rhipicephalus sanguineus, as intermediate host has been experimentally demonstrated [37]”
From Vector-borne helminths of dogs and humans in Europe (2013) · cites this paper as [37]
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- Authors
- Brianti, E., Otranto, D., Dantas-Torres, F., Weigl, S., Latrofa, M. S., Gaglio, G., Napoli, E., Brucato, G., Cauquil, L., Giannetto, S., & Bain, O.
- Affiliations
- University of Messina (IT)
- University of Bari Aldo Moro (IT)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Otranto, D.
- Venue
- Veterinary Parasitology
- Publisher
- Elsevier BV
- Published
- 2012-02
- Volume
- 183
- Issue
- 3-4
- Pages
- 330-337
- ISSN
- 0304-4017 (ISSN-L)
- OpenAlex
- W2114918249
- Cited by
- 64
- References
- 16
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 4 · 2020: 5 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 7 · 2014: 8 · 2013: 7 · 2012: 9
- FWCI
- 5.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Veterinary Parasitology (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
- Funders
- European Community (INCO-CT-2006-032321)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Life Sciences › Immunology and Microbiology › Parasitology › Vector-borne infectious diseases
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Keywords
- Rhipicephalus sanguineus, intermediate hosts, tick infestation, infective third-stage larvae
- MeSH terms
- Animals, Dog Diseases, Dogs, Filariasis, Filarioidea, Host-Parasite Interactions, Microfilariae, Molecular Sequence Data, Sicily, Skin, Sequence Analysis, DNA, Rhipicephalus sanguineus
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