Brg1 is required for murine neural stem cell maintenance and gliogenesis
Open accessAbstract
Epigenetic alterations in cell-type-specific gene expression control the transition of neural stem cells (NSCs) from predominantly neurogenic to predominantly gliogenic phases of differentiation, but how this switch occurs is unclear. Here, we show that brahma-related gene 1 (Brg1), an ATP-dependent chromatin remodeling factor, is required for the repression of neuronal commitment and the maintenance of NSCs in a state that permits them to respond to gliogenic signals. Loss of Brg1 in NSCs in conditional brg1 mutant mice results in precocious neuronal differentiation, such that cells in the ventricular zone differentiate into post-mitotic neurons before the onset of gliogenesis. As a result, there is a dramatic failure of astrocyte and oligodendrocyte differentiation in these animals. The ablation of brg1 in gliogenic progenitors in vitro also prevents growth-factor-induced astrocyte differentiation. Furthermore, proteins implicated in the maintenance of stem cells, including Sox1, Pax6 and Musashi-1, are dramatically reduced in the ventricular zones of brg1 mutant mice. We conclude that Brg1 is required to repress neuronal differentiation in NSCs as a means of permitting glial cell differentiation in response to gliogenic signals, suggesting that Brg1 regulates the switch from neurogenesis to gliogenesis.
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BRG1 is a transcription factor critical for healthy neuronal gene expression and functioning, including neuronal differentiation and the function of synapses.
“BRG1 is a transcription factor critical for healthy neuronal gene expression and functioning, including neuronal differentiation and the function of synapses [97].”
From DNA repair deficiency and senescence in concussed professional athletes involved in contact sports (2019) · cites this paper as [97]
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- Authors
- Matsumoto, S., Banine, F., Struve, J., Xing, R., Adams, C., Liu, Y., Metzger, D., Chambon, P., Rao, M. S., & Sherman, L. S.
- Affiliations
- Oregon Health & Science University (US)
- Oregon National Primate Research Center (US)
- National Institutes of Health (US)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Sherman, Larry S.
- Venue
- Developmental Biology
- Publisher
- Elsevier BV
- Published
- 2006-01
- Volume
- 289
- Issue
- 2
- Pages
- 372-383
- ISSN
- 0012-1606 (ISSN-L)
- OpenAlex
- W2030802556
- Cited by
- 147
- References
- 35
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 6 · 2023: 8 · 2022: 2 · 2021: 7 · 2020: 8 · 2019: 10 · 2018: 8 · 2017: 7 · 2016: 9 · 2015: 10 · 2014: 8 · 2013: 8 · 2012: 11
- FWCI
- 2.20
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Christopher and Dana Reeve Foundation
- National Science Council
- National Institutes of Health (7r01ns039550-04, RR 00163)
- Oregon National Primate Research Center (RR00163)
- National Institute of Neurological Disorders and Stroke (NS 39550)
- National Center for Research Resources (RR-00163.)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Keywords
- SMARCA4, neural stem cells, gliogenesis, chromatin remodeling, astrocyte differentiation, oligodendrocyte differentiation, ventricular zone, SOX1, Pax6, Musashi-1
- MeSH terms
- Animals, Astrocytes, Cell Differentiation, Cells, Cultured, Cerebral Cortex, DNA Helicases, Female, Male, Mice, Mutant Strains, Neuroglia, Neurons, Nuclear Proteins, Phenotype, Stem Cells, Transcription Factors, Signal Transduction, Gene Expression Regulation, Developmental, Cell Lineage, Organogenesis, Mice
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