Interleukin-6 released from differentiating human beige adipocytes improves browning
Open accessAbstract
Brown and beige adipocytes contribute significantly to the regulation of whole body energy expenditure and systemic metabolic homeostasis not exclusively by thermogenesis through mitochondrial uncoupling. Several studies have provided evidence in rodents that brown and beige adipocytes produce a set of adipokines ("batokines") which regulate local tissue homeostasis and have beneficial effects on physiological functions of the entire body. We observed elevated secretion of Interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1, but not tumor necrosis factor alpha (TNFα) or IL-1β pro-inflammatory cytokines, by ex vivo differentiating human beige adipocytes (induced by either PPARγ agonist or irisin) compared to white. Higher levels of IL-6, IL-8 and MCP-1 were released from human deep neck adipose tissue biopsies (enriched in browning cells) than from subcutaneous ones. IL-6 was produced in a sustained manner and mostly by the adipocytes and not by the undifferentiated progenitors. Continuous blocking of IL-6 receptor by specific antibody during beige differentiation resulted in downregulation of brown marker genes and increased morphological changes that are characteristic of white adipocytes. The data suggest that beige adipocytes adjust their production of IL-6 to reach an optimal level for differentiation in the medium enhancing browning in an autocrine manner.
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BAT is characterized by its own set of non-BAT-specific adipokines, including IL-6, IL-8, and MCP-1.
“Though most studies often focus on WAT adipokine secretion, BAT is also characterized by its own set of (non-BAT-specific) adipokines, including, for instance, IL-6, IL-8, and MCP-1 [242].”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [242]
Sustained blockage of IL-6 inhibits BAT differentiation in isolated human beige adipocytes.
“Indeed, sustained blockage of IL-6 inhibits BAT differentiation in isolated human beige adipocytes [242].”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [242]
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- Authors
- Kristóf, E., Klusóczki, Á., Veress, R., Shaw, A., Combi, Z. S., Varga, K., Győry, F., Balajthy, Z., Bai, P., Bacso, Z., & Fésüs, L.
- Affiliations
- University of Debrecen (HU)
- Corresponding author
- Fésüs, László
- ORCID
- Venue
- Experimental Cell Research
- Publisher
- Elsevier BV
- Published
- 2019-04
- Volume
- 377
- Issue
- 1-2
- Pages
- 47-55
- ISSN
- 0014-4827 (ISSN-L)
- OpenAlex
- W2917027985
- Cited by
- 77
- References
- 54
- Citations by year
- 2026: 6 · 2025: 5 · 2024: 8 · 2023: 12 · 2022: 12 · 2021: 21 · 2020: 7 · 2019: 6
- FWCI
- 7.86
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Experimental Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Repository of the Academy's Library (Library of the Hungarian Academy of Sciences) (acceptedVersion)
- University of Debrecen Electronic Archive (University of Debrecen) (submittedVersion, cc-by-nc-nd)
- University of Debrecen Electronic Archive (University of Debrecen) (submittedVersion)
- Funders
- European Commission
- Hungarian Scientific Research Fund (K123975, OTKA-NK105046)
- Magyar Tudományos Akadémia
- Emberi Eroforrások Minisztériuma
- European Regional Development Fund (GINOP-2.3.2-15-2016-00006)
- Debreceni Egyetem
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- interleukin-6, beige adipocytes, adipogenesis, IL-6 receptor inhibition, autocrine signaling, interleukin-8, MCP-1, PPARγ agonists, irisin, white adipocytes, adipokines, thermogenesis
- MeSH terms
- Adipocytes, Beige, Adult, Aged, Adipose Tissue, Brown, Cell Differentiation, Cells, Cultured, Energy Metabolism, Humans, Middle Aged, Oxygen Consumption, Interleukin-6, Interleukin-8, Chemokine CCL2, Young Adult
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