The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Analysis of endogenous cortisol concentrations in the hair of rhesus macaques
Abstract
Short-term changes in activity of the hypothalamic-pituitary-adrenocortical (HPA) system are routinely assessed by measuring glucocorticoid or metabolite concentrations in plasma, saliva, urine, or feces. However, there are no current methods for determining long-term (i.e., weeks or months) activity of this system. Herein, we describe the development and validation of a simple procedure for measuring cortisol concentrations in the hair of rhesus macaques. This procedure involves two brief isopropanol washes of the hair strands to remove surface contaminants, subsequent powdering of the washed and dried hair, a 24-h methanol extraction followed by evaporation of the solvent and reconstitution of the extract in assay buffer, and finally analysis of the extracted cortisol by a sensitive and specific enzyme immunoassay. Our results confirm the specificity of the procedure for cortisol, show that proximal and distal segments of hair do not differ in their cortisol concentration, and demonstrate that a significant and prolonged stressful experience produces a significant increase in hair cortisol. This new procedure should be valuable for assessing baseline HPA activity in nonhuman primates (and, with appropriate validation, in other species as well) over relatively long periods of time, and also for monitoring chronic stress that might be associated with various experimental manipulations.
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In the citing paper
“providing prospective validation of the hair cortisol method as a field-friendly biological marker of stress exposure”
From Chronic Stress, Hair Cortisol and Depression: A Prospective and Longitudinal Study of Medical Internship (2018) · cites this paper as Davenport et al., 2006
Citations
Cites
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Salivary cortisol in psychoneuroendocrine research: recent developments and applications
Kirschbaum, C., & Hellhammer, D. H. (1994) · Psychoneuroendocrinology · doi:10.1016/0306-4530(94)90013-2 · Cited by 2,107
DUNN, J. F., NISULA, B. C., & RODBARD, D. (1981) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jcem-53-1-58 · Cited by 1,238
Potential problems with the interpretation of hair analysis results
Wennig, R. (2000) · Forensic Science International · doi:10.1016/s0379-0738(99)00146-2 · Cited by 677
Hair Analysis for Drugs of Abuse
Baumgartner, W., Hill, V., & BLAHD, W. H. (1989) · Journal of Forensic Sciences · doi:10.1520/jfs12787j · Cited by 473
Anatomy and physiology of hair
Harkey, M. R. (1993) · Forensic Science International · doi:10.1016/0379-0738(93)90255-9 · Cited by 380
Detection of physiological concentrations of cortisol and cortisone in human hair
Raul, J.-S., Cirimele, V., Ludes, B., & Kintz, P. (2004) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2004.02.010 · Cited by 348
Cirimele, V., Kintz, P., Dumestre-Toulet, V., Goullé, J., & Ludes, B. (2000) · Forensic Science International · doi:10.1016/s0379-0738(99)00180-2 · Cited by 190
Cone, E. J., Yousefnejad, D., Darwin, W. D., & Maguire, T. (1991) · Journal of Analytical Toxicology · doi:10.1093/jat/15.5.250 · Cited by 187
Research works printed but not linked: 1
- Lutz, 2005, The effect of relocation on nighttime activity and salivary cortisol in singly-housed male Macaca mulatta with and without a record of self-injury, Am. J. Primatol., 66, 83
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Russell, E., Koren, G., Rieder, M., & Van Uum, S. (2012) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2011.09.009 · Cited by 1,063
“In rhesus macaques, in contrast, there was a significant correlation between the cortisol concentration in saliva and that in hair (r = 0.797, P 0.001) (Davenport et al., 2006).”
BackgroundMethodInfluentialMeasuring stress in wildlife: techniques for quantifying glucocorticoids
Sheriff, M. J., Dantzer, B., Delehanty, B., Palme, R., & Boonstra, R. (2011) · Oecologia · doi:10.1007/s00442-011-1943-y · Cited by 919
“Exact procedures vary, but all typically involve a long incubation time (16–24 h) at various temperatures (20–52 C), with or without agitation (Davenport et al. 2006; Sauvé et al. 2007; Accorsi et al. 2008; Sharpley et al. 2009).”
BackgroundInfluentialAnalysis of cortisol in hair – State of the art and future directions
Stalder, T., & Kirschbaum, C. (2012) · Brain Behavior and Immunity · doi:10.1016/j.bbi.2012.02.002 · Cited by 835
“, 2009; Dettenborn et al., 2010; Gao et al., 2010; Steudte et al., 2011b; Skoluda et al., in press; Xie et al., in press; Stalder et al., under review) but not by others (e.g., Thomson et al., 2010; Manenschijn et al., 2011). Interestingly, Manenschijn et al. (2011) noted that those studies not observing a declining p…”
BackgroundKirschbaum, C., Tietze, A., Skoluda, N., & Dettenborn, L. (2009) · Psychoneuroendocrinology · doi:10.1016/j.psyneuen.2008.08.024 · Cited by 584
“Moreover, Davenport et al. (2006) published the methodological details and validation of a simple procedure for measurements of endogenous cortisol concentrations in rhesus monkey hair with a special focus on the appropriate wash procedure.”
InfluentialMeasurement of cortisol in human Hair as a biomarker of systemic exposure
Koren, G., Grace Walsh, R. N., Tokmakejian, S., & Uum, S. H. M. V. (2007) · Clinical and investigative medicine · doi:10.25011/cim.v30i5.2894 · Cited by 578
Metadata
- Authors
- Davenport, M. D., Tiefenbacher, S., Lutz, C. K., Novak, M. A., & Meyer, J. S.
- Affiliations
- Harvard University (US)
- University of Massachusetts Amherst (US)
- Corresponding author
- Meyer, Jerrold S.
- Venue
- General and Comparative Endocrinology
- Publisher
- Elsevier BV
- Published
- 2006-07
- Volume
- 147
- Issue
- 3
- Pages
- 255-261
- ISSN
- 0016-6480 (ISSN-L)
- OpenAlex
- W2074364102
- Cited by
- 659
- References
- 31
- Citations by year
- 2026: 18 · 2025: 29 · 2024: 33 · 2023: 41 · 2022: 35 · 2021: 53 · 2020: 44 · 2019: 73 · 2018: 33 · 2017: 52 · 2016: 53 · 2015: 35 · 2014: 31 · 2013: 28 · 2012: 30
- FWCI
- 3.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- General and Comparative Endocrinology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Center for Research Resources (#RR11122, RR00 168, R24RR011122)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Behavior and Welfare Studies
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- hair cortisol, rhesus monkeys, hypothalamic-pituitary-adrenal axis, chronic stress, non-human primates, stress biomarkers, enzyme immunoassay validation
- MeSH terms
- Animals, Hair, Hydrocortisone, Immunoenzyme Techniques, Macaca mulatta, Male, Saliva, Sensitivity and Specificity, Skin, Stress, Physiological, Time Factors
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