Lyophilization provides long-term stability for a lipid nanoparticle-formulated, nucleoside-modified mRNA vaccine
Open accessAbstract
Lipid nanoparticle (LNP)-formulated nucleoside-modified mRNA vaccines have proven to be very successful in the fight against the coronavirus disease 2019 (COVID-19) pandemic. They are effective, safe, and can be produced in large quantities. However, the long-term storage of mRNA-LNP vaccines without freezing is still a challenge. Here, we demonstrate that nucleoside-modified mRNA-LNPs can be lyophilized, and the physicochemical properties of the lyophilized material do not significantly change for 12 weeks after storage at room temperature and for at least 24 weeks after storage at 4°C. Importantly, we show in comparative mouse studies that lyophilized firefly luciferase-encoding mRNA-LNPs maintain their high expression, and no decrease in the immunogenicity of a lyophilized influenza virus hemagglutinin-encoding mRNA-LNP vaccine was observed after 12 weeks of storage at room temperature or for at least 24 weeks after storage at 4°C. Our studies offer a potential solution to overcome the long-term storage-related limitations of nucleoside-modified mRNA-LNP vaccines.
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Lyophilization of modified mRNA-LNP provides long-term stability at room temperature.
“Furthermore, lyophilization of modified mRNA-LNP provides long-term stability at room temperature (56).”
From mRNA vaccine with unmodified uridine induces robust type I interferon-dependent anti-tumor immunity in a melanoma model (2022) · cites this paper as [56]
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- Authors
- Muramatsu, H., Lam, K., Bajusz, C., Laczkó, D., Karikó, K., Schreiner, P., Martin, A., Lutwyche, P., Heyes, J., & Pardi, N.
- Affiliations
- University of Pennsylvania (US)
- HUN-REN Szegedi Biológiai Kutatóközpont (HU)
- BioNTech (Germany) (DE)
- Corresponding author
- Pardi, Norbert
- ORCID
- Venue
- Molecular Therapy
- Publisher
- Elsevier BV
- Published
- 2022-05
- Volume
- 30
- Issue
- 5
- Pages
- 1941-1951
- ISSN
- 1525-0016 (ISSN-L)
- PMC
- PMC8815268
- OpenAlex
- W4210741764
- Cited by
- 331
- References
- 21
- Citations by year
- 2026: 80 · 2025: 95 · 2024: 76 · 2023: 60 · 2022: 20
- FWCI
- 20.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Molecular Therapy (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Repository of the Academy's Library (Library of the Hungarian Academy of Sciences) (acceptedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (R01AI146101, R01AI153064)
- University of Pennsylvania
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- freeze-drying, nucleoside-modified mRNA, room temperature storage, physicochemical stability, immunogenicity, COVID-19 vaccines
- MeSH terms
- COVID-19, mRNA Vaccines, Animals, Freeze Drying, Influenza Vaccines, Liposomes, Nucleosides, RNA, Messenger, Vaccines, Synthetic, Mice, Nanoparticles
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