Co-localization of vasoactive intestinal polypeptide, $γ$-aminobutyric acid and choline acetyltransferase in neocortical interneurons of the adult rat
Abstract
Interneurons immunoreactive for vasoactive intestinal polypeptide (VIP) are integral elements of columnar organization patterns in the rat cerebral cortex. By application of the sensitive mirror technique, the co-localization of VIP with the classical inhibitory neurotransmitter gamma-aminobutyric acid (GABA) and the acetylcholine-synthesizing enzyme, choline acetyltransferase (ChAT), was investigated in neocortical neurons. Furthermore, the frequency of co-localization of ChAT with GABA was determined. In a sample of 118 VIP-immunoreactive neurons, mostly from the primary somatosensory cortex, it was demonstrated that virtually all of them reveal immunoreactivity for GABA and, therefore, are to be GABAergic. Moreover, 34% of mostly bipolar, VIP-positive neurons contained ChAT and are, thus, supposedly cholinergic as well. Co-localization of VIP and ChAT varied according to cortical laminae. Finally, 88% of a total of 60 ChAT-immunoreactive neurons were also immunostained for GABA. It is concluded that almost all VIP-immunoreactive neurons and most of the cholinergic neurons in rat neocortex represent partly overlapping subpopulations of inhibitory interneurons utilizing GABA.
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Bipolar cortical interneurons expressing VIP and ChAT were first identified by immunohistochemistry in rodents and account for about one third of all VIP+ cortical neurons.
“Bipolar cortical interneurons expressing vasoactive intestinal polypeptide (VIP) and ChAT were first identified using immunohistochemistry in rodents and account for $1/3 of all VIP+ cortical neurons.92,93”
From Synaptic and circuit functions of multitransmitter neurons in the mammalian brain (2023) · cites this paper as [92]
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- Authors
- Bayraktar, T., Staiger, J. F., Acsady, L., Cozzari, C., Freund, T. F., & Zilles, K.
- Affiliations
- Heinrich Heine University Düsseldorf (DE)
- HUN-REN Institute of Experimental Medicine (HU)
- Corresponding author
- Staiger, J.F
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1997-05
- Volume
- 757
- Issue
- 2
- Pages
- 209-217
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2046281188
- Cited by
- 99
- References
- 45
- Citations by year
- 2024: 5 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 5 · 2014: 6 · 2013: 3 · 2012: 2
- FWCI
- 1.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- vasoactive intestinal peptide, GABA, choline acetyltransferase, neocortical interneurons, colocalization, GABAergic neurons, cholinergic neurons, primary somatosensory cortex, cortical layers, columnar organization, inhibitory neurotransmitter, rats
- MeSH terms
- Animals, Cerebral Cortex, Choline O-Acetyltransferase, gamma-Aminobutyric Acid, Immunohistochemistry, Interneurons, Male, Tissue Distribution, Vasoactive Intestinal Peptide, Rats, Wistar, Rats
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