Pharmacological sensitivity and gene expression analysis of the tibial nerve injury model of neuropathic pain
Abstract
The tibial nerve injury model is a novel, surgically uncomplicated, rat model of neuropathic pain based on a unilateral transection (neurotomy) of the tibial branch of the sciatic nerve. The aim of the present study was to describe some behavioral and molecular features of the model, and to test its sensitivity to a number of drugs which are currently used for the treatment of neuropathic pain. The model was characterized by a pronounced mechanical allodynia which was present in all subjects and a less robust thermal hyperalgesia. Mechanical allodynia developed within 2 weeks post-surgery and was reliably present for at least 9 weeks. Neurotomized rats showed no autotomy and their body weight developed normally. Gene expression in ipsilateral L5 dorsal root ganglia, analyzed by quantitative polymerase chain reaction (PCR), showed a pronounced up-regulation of galanin and vasointestinal peptide (VIP). This up-regulation developed rapidly (within 1 to 2 days following neurotomy) and remained present for at least 12 days. On the other hand, expression of calcitonin gene-related peptide (CGRP) and substance P mRNA was down-regulated 12 days following neurotomy. Mechanical allodynia was completely reversed by morphine [minimal effective dose (MED): 8 mg/kg, i.p.] and partially reversed by carbamazepine (MED: 64 mg/kg, i.p.), baclofen (MED: 3 mg/kg, i.p.) and amitriptyline (trend for efficacy at 32 mg/kg, i.p.), but not by gabapentin (50-100 mg/kg, i.p.). The finding that the tibial nerve injury model shows a robust and persistent mechanical allodynia which is sensitive to a number of established analgesics, as well as a gene expression profile which is compatible with that obtained in other models of neuropathic pain, further supports its validity as a reliable and surgically uncomplicated model for the study of neuropathic pain.
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Tibial nerve injury produces mechanical allodynia and thermal hyperalgesia.
“Mechanical allodynia and thermal hyperalgesia were present in the initial study describing this technique105 as well as the study we reviewed.”
From Cannabinoids as Pharmacotherapies for Neuropathic Pain: From the Bench to the Bedside (2009) · cites this paper as [105]
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- Authors
- Hofmann, H. A., De Vry, J., Siegling, A., Spreyer, P., & Denzer, D.
- Affiliations
- Bayer (Germany) (DE)
- Corresponding author
- Denzer, Dirk
- Venue
- European Journal of Pharmacology
- Publisher
- Elsevier BV
- Published
- 2003-05
- Volume
- 470
- Issue
- 1-2
- Pages
- 17-25
- ISSN
- 0014-2999 (ISSN-L)
- OpenAlex
- W2053875152
- Cited by
- 70
- References
- 50
- Citations by year
- 2026: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 5 · 2015: 4 · 2014: 6 · 2013: 6 · 2012: 2
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- European Journal of Pharmacology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Health Sciences › Medicine › Anesthesiology and Pain Medicine › Pain Management and Treatment
- Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
- Keywords
- neuropathic pain, mechanical allodynia, thermal hyperalgesia, galanin, vasoactive intestinal peptide, calcitonin gene-related peptide, substance P, analgesic sensitivity, morphine, carbamazepine, baclofen, amitriptyline, gabapentin
- MeSH terms
- Analgesics, Animals, Disease Models, Animal, Gene Expression Regulation, Male, Tibial Nerve, Rats, Wistar, Pain Threshold, Tibial Neuropathy, Rats
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