Xanthine oxidoreductase is asymmetrically localised on the outer surface of human endothelial and epithelial cells in culture
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Subcellular localisation of xanthine oxidoreductase (XOR) was determined by indirect immunofluorescence using confocal microscopy in human endothelial and epithelial cell lines and in primary cultures of human umbilical vein endothelial cells. XOR was diffusely distributed throughout the cytoplasm but with higher intensity in the perinuclear region. In non‐permeabilised cells, XOR was clearly seen to be asymmetrically located on the outer surfaces, showing, in many cases, a higher intensity on those faces apposed by closely neighbouring cells. Such specific distribution suggests a functional role for the enzyme in cell‐cell interactions, possibly involving signalling via reactive oxygen species
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Many studies show that BH4 levels correlate with eNOS activity.
“Many studies have shown that BH4 levels correlate with eNOS activity (796, 1006).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [796]
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Hyperuricemia, Type 2 Diabetes and Insulin Resistance: Role of Nitric Oxide
Bahadoran, Z., Mirmiran, P., Kashfi, K., & Ghasemi, A. (2022) · BENTHAM SCIENCE PUBLISHERS eBooks · doi:10.2174/9789815079814122010012
Massimo, G., Khambata, R. S., Chapman, T., Birchall, K., Shabbir, A., Dyson, N., Rathod, K. S., Borghi, C., & Ahluwalia, A. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.03.24.533749
The nitrite reductase activity of xanthine oxidoreductase sustains cardiovascular healthPreprint
Dyson, N., Khambata, R. S., Parakaw, T., Massimo, G., Khuat, N. H., Noor, A. A., Gee, L. C., Lim, I., Siddique, U., Sullivan, A. J., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.11.01.621390
REGULATION OF THE HUMAN XANTHINE OXIDOREDUCTASE GENE
Martelin, E. (2004) · Työväentutkimus Vuosikirja
- Authors
- Rouquette, M., Page, S., Bryant, R., Benboubetra, M., Stevens, C. R., Blake, D. R., Whish, W. D., Harrison, R., & Tosh, D.
- Affiliations
- University of Bath (GB)
- Corresponding author
- Harrison, Roger
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1998-04-24
- Volume
- 426
- Issue
- 3
- Pages
- 397-401
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1991707890
- Cited by
- 78
- References
- 25
- Citations by year
- 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 2 · 2012: 2
- FWCI
- 3.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Nephrology › Gout, Hyperuricemia, Uric Acid
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Keywords
- xanthine oxidoreductase, subcellular localization, cell-cell interactions, endothelial cells, epithelial cells, human umbilical vein endothelial cells, reactive oxygen species, signal transduction, perinuclear region
- MeSH terms
- Cell Line, Cell Membrane, Cytoplasm, Endothelium, Endothelium, Vascular, Epithelial Cells, Extracellular Space, Humans, Umbilical Veins, Xanthine Oxidase
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