Cited by 78Gout, Hyperuricemia, Uric AcidRedox biology and oxidative stressNitric Oxide and Endothelin Effects

Xanthine oxidoreductase is asymmetrically localised on the outer surface of human endothelial and epithelial cells in culture

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Rouquette, Magali, Page, Susanna, Bryant, Richard, Benboubetra, Mustapha, Stevens, Cliff R, Blake, David R, Whish, William D, Harrison, Roger +1 more·1998·FEBS Letters

Abstract

Subcellular localisation of xanthine oxidoreductase (XOR) was determined by indirect immunofluorescence using confocal microscopy in human endothelial and epithelial cell lines and in primary cultures of human umbilical vein endothelial cells. XOR was diffusely distributed throughout the cytoplasm but with higher intensity in the perinuclear region. In non‐permeabilised cells, XOR was clearly seen to be asymmetrically located on the outer surfaces, showing, in many cases, a higher intensity on those faces apposed by closely neighbouring cells. Such specific distribution suggests a functional role for the enzyme in cell‐cell interactions, possibly involving signalling via reactive oxygen species

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Research works printed but not linked: 6

  1. Bray, R.C. (1975) in: The Enzymes (Boyer, P.D., Ed.), Vol XII, 3rd edn., pp 299^419, Academic Press, New York.
  2. Harrison, R. (1997) Biochem. Soc. Trans. 25, 786^791.
  3. Edgell, W.R., McDonald, C.C. and Graham, J.B. (1983) Proc. Natl. Acad. Sci. USA 80, 3734^3737.
  4. Rouquette, M., Stevens, C.R., Blake, D.R., Harrison, R., Whish, J. and Whish, W.D.H. (1997) Biochem. Soc. Trans. 25, 532S.
  5. Page, S., Powell, D., Benboubetra, M., Stevens, C.R., Blake, D.R., Selase, F., Wolstenholme, A. and Harrison, R. (1998) Bio- chim. Biophys. Acta (in press).
  6. Beckman, J.S., Parks, D.A., Pearson, J.D., Marshall, P.A. and Freeman, B.A. (1989) Free Radical Biol. Med. 6, 607^615.

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Authors
Rouquette, M., Page, S., Bryant, R., Benboubetra, M., Stevens, C. R., Blake, D. R., Whish, W. D., Harrison, R., & Tosh, D.
Affiliations
University of Bath (GB)
Corresponding author
Harrison, Roger
Venue
FEBS Letters
Publisher
Wiley
Published
1998-04-24
Volume
426
Issue
3
Pages
397-401
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W1991707890
Cited by
78
References
25
Citations by year
2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 2 · 2012: 2
FWCI
3.35
Type
journal article
Language
en
Open access
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Nephrology › Gout, Hyperuricemia, Uric Acid
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
xanthine oxidoreductase, subcellular localization, cell-cell interactions, endothelial cells, epithelial cells, human umbilical vein endothelial cells, reactive oxygen species, signal transduction, perinuclear region
MeSH terms
Cell Line, Cell Membrane, Cytoplasm, Endothelium, Endothelium, Vascular, Epithelial Cells, Extracellular Space, Humans, Umbilical Veins, Xanthine Oxidase

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