Cited by 68Ion channel regulation and functionNitric Oxide and Endothelin EffectsCardiac electrophysiology and arrhythmias

Inhibition of skeletal muscle sarcoplasmic reticulum Ca2+‐ATPase by nitric oxide

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Ishii, Toshiaki, Sunami, Osamu, Saitoh, Noriko, Nishio, Hideaki, Takeuchi, Tadayoshi, Hata, Fumiaki·1998·FEBS Letters

Abstract

The effects of nitric oxide on the activities of thapsigargin-sensitive sarcoplasmic reticulum Ca2+-ATPase (SERCA) and Ca2+ uptake by sarcoplasmic reticulum (SR) membranes prepared from white skeletal muscle of rabbit femoral muscle were studied. Pretreatment of the SR preparations with nitric oxide at concentrations of up to 250 microM for 1 min decreased the SERCA activity concentration dependently, and also decreased their Ca2+ uptake. Both these effects of nitric oxide were reversible. Inhibitors of guanylyl cyclase and protein kinase G (PKG) had no significant effect on the nitric oxide-induced inhibitions of SERCA and Ca2+ uptake. Moreover, dithiothreitol did not reverse the inhibitory effects of nitric oxide on SERCA and Ca2+ uptake. These findings suggest that nitric oxide inhibits SERCA, mainly SERCA 1, of rabbit femoral skeletal muscle by an action independent of the cyclic GMP-PKG system or oxidation of thiols, and probably by a direct action on SERCA protein.

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  • Various contractile-related proteins can undergo reversible post-translation modifications at cysteine residues on thiols, such as myosin, troponin, SERCA and ryanodine receptors, and these modifications are likely dependent on interactions between NO, reactive oxygen species and glutathione bioavailability.

    “The potential influence of S-nitrosylation on excitation–contraction coupling is complex given that various contractile-related proteins can undergo reversible post-translation modifications at cysteine residues on thiols, such as myosin [140], troponin [141], SERCA [142] and ryanodine receptors (RyRs) [143,144], and that these post-translation protein modifications are likely dependent on inter”

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Research works printed but not linked: 3

  1. Bredt, D.S. and Snyder, S.H. (1990) Proc. Natl. Acad. Sci. USA 87, 682^685.
  2. Ishii, T. and Takeyasu, K. (1993) Proc. Natl. Acad. Sci. USA 90, 8881^8885.
  3. Fucci, L., Oliver, C.N., Coon, M.J. and Stadtman, E.R. (1983) Proc. Natl. Acad. Sci. USA 80, 1521^1525.

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Authors
Ishii, T., Sunami, O., Saitoh, N., Nishio, H., Takeuchi, T., & Hata, F.
Affiliations
Osaka Prefecture University (JP)
Corresponding author
Hata, Fumiaki
Venue
FEBS Letters
Publisher
Wiley
Published
1998-11-27
Volume
440
Issue
1-2
Pages
218-222
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W2030979507
Cited by
68
References
30
Citations by year
2025: 1 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 5 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 5 · 2014: 3 · 2013: 5
FWCI
0.93
Type
journal article
Language
en
Open access
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
Keywords
nitric oxide, sarcoplasmic reticulum Ca2+-ATPase, SERCA, SERCA1, calcium uptake, skeletal muscle, femoral muscle, enzyme inhibition, guanylate cyclase, protein kinase G, thiol oxidation, cyclic GMP
MeSH terms
Calcium-Transporting ATPases, Sodium-Potassium-Exchanging ATPase, Aminoquinolines, Animals, Caffeine, Calcium, Calcium Channel Agonists, Calcium Channel Blockers, Dithiothreitol, Cyclic GMP, Guanylate Cyclase, Heparin, Nitric Oxide, Ouabain, Rabbits, Ryanodine, Sarcoplasmic Reticulum, Thionucleotides, Inositol 1,4,5-Trisphosphate, Cyclic GMP-Dependent Protein Kinases, Thapsigargin

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