Inhibition of skeletal muscle sarcoplasmic reticulum Ca2+‐ATPase by nitric oxide
Open accessAbstract
The effects of nitric oxide on the activities of thapsigargin-sensitive sarcoplasmic reticulum Ca2+-ATPase (SERCA) and Ca2+ uptake by sarcoplasmic reticulum (SR) membranes prepared from white skeletal muscle of rabbit femoral muscle were studied. Pretreatment of the SR preparations with nitric oxide at concentrations of up to 250 microM for 1 min decreased the SERCA activity concentration dependently, and also decreased their Ca2+ uptake. Both these effects of nitric oxide were reversible. Inhibitors of guanylyl cyclase and protein kinase G (PKG) had no significant effect on the nitric oxide-induced inhibitions of SERCA and Ca2+ uptake. Moreover, dithiothreitol did not reverse the inhibitory effects of nitric oxide on SERCA and Ca2+ uptake. These findings suggest that nitric oxide inhibits SERCA, mainly SERCA 1, of rabbit femoral skeletal muscle by an action independent of the cyclic GMP-PKG system or oxidation of thiols, and probably by a direct action on SERCA protein.
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Various contractile-related proteins can undergo reversible post-translation modifications at cysteine residues on thiols, such as myosin, troponin, SERCA and ryanodine receptors, and these modifications are likely dependent on interactions between NO, reactive oxygen species and glutathione bioavailability.
“The potential influence of S-nitrosylation on excitation–contraction coupling is complex given that various contractile-related proteins can undergo reversible post-translation modifications at cysteine residues on thiols, such as myosin [140], troponin [141], SERCA [142] and ryanodine receptors (RyRs) [143,144], and that these post-translation protein modifications are likely dependent on inter”
From Effects of Dietary Nitrate Supplementation on Weightlifting Exercise Performance in Healthy Adults: A Systematic Review (2020) · cites this paper as [142]
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- Authors
- Ishii, T., Sunami, O., Saitoh, N., Nishio, H., Takeuchi, T., & Hata, F.
- Affiliations
- Osaka Prefecture University (JP)
- Corresponding author
- Hata, Fumiaki
- ORCID
- Ishii, Toshiaki
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1998-11-27
- Volume
- 440
- Issue
- 1-2
- Pages
- 218-222
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2030979507
- Cited by
- 68
- References
- 30
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 1 · 2020: 5 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 5 · 2014: 3 · 2013: 5
- FWCI
- 0.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Keywords
- nitric oxide, sarcoplasmic reticulum Ca2+-ATPase, SERCA, SERCA1, calcium uptake, skeletal muscle, femoral muscle, enzyme inhibition, guanylate cyclase, protein kinase G, thiol oxidation, cyclic GMP
- MeSH terms
- Calcium-Transporting ATPases, Sodium-Potassium-Exchanging ATPase, Aminoquinolines, Animals, Caffeine, Calcium, Calcium Channel Agonists, Calcium Channel Blockers, Dithiothreitol, Cyclic GMP, Guanylate Cyclase, Heparin, Nitric Oxide, Ouabain, Rabbits, Ryanodine, Sarcoplasmic Reticulum, Thionucleotides, Inositol 1,4,5-Trisphosphate, Cyclic GMP-Dependent Protein Kinases, Thapsigargin
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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