The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Concentrations of cyclic AMP-dependent protein kinase subunits in various tissues.
Open accessAbstract
The concentrations of the regulatory (R) and catalytic (C) subunits of adenosine 3':5'-monophosphate (cyclic AMP)-dependent protein kinase(s) were measured in extracts of skeletal muscle, heart, liver, kidney, and brain. These concentrations were also estimated for the particulate fraction from brain, the only tissue in which a major part of the total activity was not readily extracted in a soluble form. Values for R were determined by measuring the amount of cyclic [3H]amp bound to protein in these tissue fractions under specified conditions; it was assumed that 1 mol of cyclic AMP binds to 1 mol of R. Values for C were determined from measurements of the specific protein kinase activity of the fractions utilizing the turnover number of pure C in the calculations. Turnover numbers for C were found to be identical for this subunit obtained in the pure form from rabbit skeletal muscle, rabbit liver, and beef heart. The methods used for measuring C were evaluated by kinetic studies and through the use of the specific heatstable protein inhibitor of cyclic AMP-dependent protein kinase(s). R and C were found to exist in a 1:1 molar ratio in all of the tissue fractions that were studied. the absolute concentrations of R and C ranged from 0.23 mumol/kg wet weight for liver to 0.78 mumol/kg wet weight for brain. For brain this value was based on the amount of each subunit in the particulate as well as the soluble fraction. For other tissues the values were based solely on the subunit content of the latter fraction. It was noted that the molar concentrations of R are close to those of cyclic AMP under basal conditions in the various tissues.
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Weber, K., & Osborn, M. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)94333-4 · Cited by 20,298
DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION
Gornall, A. G., Bardawill, C. J., & David, M. M. (1949) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)57021-6 · Cited by 16,158
A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate
Gilman, A. G. (1970) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.67.1.305 · Cited by 3,249
A simple method for the preparation of 32P-labelled adenosine triphosphate of high specific activity
Glynn, I., & Chappell, J. (1964) · Biochemical Journal · doi:10.1042/bj0900147 · Cited by 1,876
Measurement of protein-binding phenomena by gel filtration
Hummel, J. P., & Dreyer, W. J. (1962) · Biochimica et Biophysica Acta · doi:10.1016/0006-3002(62)90124-5 · Cited by 1,279
Studies on histones. 7. Preparative methods for histone fractions from calf thymus
Johns, E. (1964) · Biochemical Journal · doi:10.1042/bj0920055 · Cited by 1,038
Walsh, D. A., Ashby, C. D., Gonzalez, C., Calkins, D., Fischer, E. H., & Krebs, E. G. (1971) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)77177-4 · Cited by 887
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Hofmann, F., Beavo, J., Bechtel, P., & Krebs, E. (1975) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)40885-5 · Cited by 563
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Kameyama, M., Hofmann, F., & Trautwein, W. (1985) · Pfl�gers Archiv European Journal of Physiology · doi:10.1007/bf00582573 · Cited by 393
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Chain, D. G., Casadio, A., Schacher, S., Hegde, A. N., Valbrun, M., Yamamoto, N., Goldberg, A. L., Bartsch, D., Kandel, E. R., & Schwartz, J. H. (1999) · Neuron · doi:10.1016/s0896-6273(00)80686-8 · Cited by 186
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Metadata
- Authors
- Hofmann, F., Bechtel, P. J., & Krebs, E. G.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1977-02
- Volume
- 252
- Issue
- 4
- Pages
- 1441-1447
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1625449763
- Cited by
- 267
- References
- 20
- FWCI
- 8.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
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