Cited by 186Neuroscience and Neuropharmacology ResearchMemory and Neural MechanismsProtein Kinase Regulation and GTPase Signaling

Mechanisms for Generating the Autonomous cAMP-Dependent Protein Kinase Required for Long-Term Facilitation in Aplysia

Open access
Chain, Daniel G, Casadio, Andrea, Schacher, Samuel, Hegde, Ashok N, Valbrun, Mireille, Yamamoto, Naoki, Goldberg, Alfred L, Bartsch, Dusan +2 more·1999·Neuron

Abstract

The formation of a persistently active cAMP-dependent protein kinase (PKA) is critical for establishing long-term synaptic facilitation (LTF) in Aplysia. The injection of bovine catalytic (C) subunits into sensory neurons is sufficient to produce protein synthesis-dependent LTF. Early in the LTF induced by serotonin (5-HT), an autonomous PKA is generated through the ubiquitin-proteasome-mediated proteolysis of regulatory (R) subunits. The degradation of R occurs during an early time window and appears to be a key function of proteasomes in LTF. Lactacystin, a specific proteasome inhibitor, blocks the facilitation induced by 5-HT, and this block is rescued by injecting C subunits. R is degraded through an allosteric mechanism requiring an elevation of cAMP coincident with the induction of a ubiquitin carboxy-terminal hydrolase.

Integrity signals

Registry

Crossref · 10.1016/s0896-6273(00)80686-8

Cites

69 of 69 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The PKA and ERK cascades are necessary to induce long-term synaptic facilitation (LTF) in Aplysia.

    “Two biochemical cascades required for LTF are those that mediate activation of protein kinase A (PKA)12–15 and the MAP kinase isoform termed extracellular signal-regulated kinase (ERK)16–18.”

    From Computational design of enhanced learning protocols (2012) · cites this paper as [15]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Chain, D. G., Casadio, A., Schacher, S., Hegde, A. N., Valbrun, M., Yamamoto, N., Goldberg, A. L., Bartsch, D., Kandel, E. R., & Schwartz, J. H.
Affiliations
  • Columbia University (US)
  • Harvard University (US)
Corresponding author
Schwartz, James H
Venue
Neuron
Publisher
Elsevier BV
Published
1999-01
Volume
22
Issue
1
Pages
147-156
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2021608362
Cited by
186
References
69
Citations by year
2025: 2 · 2024: 5 · 2023: 3 · 2022: 6 · 2021: 1 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 6 · 2016: 8 · 2015: 10 · 2014: 14 · 2013: 5 · 2012: 6
FWCI
3.74
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p01gm032099-14)
  • National Institute of Mental Health (MH-48850, R01MH048850)
  • National Institute of General Medical Sciences (GM 32099)
  • National Institute of Neurological Disorders and Stroke (NS-29255)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Keywords
protein kinase A, long-term facilitation, Aplysia, ubiquitin-proteasome degradation, catalytic subunit, serotonin, synaptic plasticity, lactacystin, allosteric regulation, sensory neurons
MeSH terms
Cyclic AMP, Animals, Aplysia, Cattle, Cysteine Endopeptidases, Injections, Multienzyme Complexes, Neurons, Afferent, Peptide Fragments, Serotonin, Ubiquitins, Long-Term Potentiation, Cyclic AMP-Dependent Protein Kinases, Proteasome Endopeptidase Complex

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review