Apolipoprotein E isoform phenotyping methodology and population frequency with identification of apoE1 and apoE5 isoforms
Open accessAbstract
Minigel methodology has been utilized for apolipoprotein (apo) E isoform phenotyping and criteria for distinguishing the apoE4/4 phenotype (mean apoE4/apoE3 ratio: 5.81) from the apoE4/3 phenotype (mean ratio: 1.01) and the apoE3/3 phenotype (mean apoE3/apoE2 ratio: 2.67) from the apoE3/2 phenotype (mean ratio: 0.76) based on gel scanning were developed. ApoE allele frequencies in 1209 subjects were: apoE3, 0.786; apoE4, 0.135; apoE2, 0.075; apoE5, 0.002; and apoE1, 0.002. Subjects with the apoE2 allele tended to have higher plasma very low density lipoprotein (VLDL) cholesterol and lower low density lipoprotein (LDL) cholesterol concentrations than subjects with the apoE3 allele, while the converse was true for subjects with the apoE4 allele. Subjects with the rare apoE1 allele had values similar to those with the apoE2 allele, while subjects with the rare apoE5 allele had values similar to those with the apoE4 allele.
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Individuals with apoE5 develop type II HLP and vulnerability to atherosclerosis due to hypercholesterolemia.
“individuals with apoE5 develop type II HLP [8] and vulnerability to atherosclerosis due to hypercholesterolemia [9].”
From A case of lipoprotein glomerulopathy with a rare apolipoprotein E isoform combined with neurofibromatosis type I (2018) · cites this paper as [8]
The prevalence of apoE5 is 0.2% (E3 78.6%, E4 13.5%, E2 7.5%).
“The prevalence of apoE5 has been reported as 0.2% (E3, 78.6%; E4, 13.5%; E2, 7.5%)”
From A case of lipoprotein glomerulopathy with a rare apolipoprotein E isoform combined with neurofibromatosis type I (2018) · cites this paper as [8]
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- Authors
- Ordovas, J. M., Litwack-Klein, L., Wilson, P. W., Schaefer, M. M., & Schaefer, E. J.
- Affiliations
- Tufts University (US)
- Venue
- Journal of Lipid Research
- Publisher
- Elsevier BV
- Published
- 1987-04
- Volume
- 28
- Issue
- 4
- Pages
- 371-380
- ISSN
- 0022-2275 (ISSN-L)
- OpenAlex
- W2285849357
- Cited by
- 238
- References
- 7
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2023: 8 · 2022: 2 · 2021: 6 · 2020: 3 · 2019: 2 · 2018: 4 · 2017: 5 · 2016: 2 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 15.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Lipid Research (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Heart, Lung, and Blood Institute (RFP NHLBI HV 83-03)
- Topics
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- apolipoprotein E, allele frequency, APOE ε4, APOE2, APOE3, VLDL cholesterol, LDL cholesterol, plasma lipids
- MeSH terms
- Adult, Alleles, Apolipoproteins E, Female, Gene Frequency, Humans, Hyperlipoproteinemias, Lipoproteins, LDL, Lipoproteins, VLDL, Male, Middle Aged, Phenotype
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