mRNA and apolipoprotein E synthesis abnormalities in peripheral blood monocyte macrophages in familial apolipoprotein E deficiency.
Open accessAbstract
We have studied synthesis of apolipoprotein E (apo-E) and apo-E mRNA in cultures of peripheral blood human monocyte macrophages (M-M cultures) obtained from a patient with familial apolipoprotein E deficiency. We have found that the M-M cultures of the apo-E-deficient patients contained two apo-E mRNA species with slightly different molecular weight as compared to normal apo-E mRNA. The apo-E mRNA concentration of the apo-E-deficient cultures was approximately 50-fold reduced as compared to the normal cultures, whereas the actin mRNA concentrations were identical in both M-M cultures. Genomic blotting analysis using a full-length apo-E cDNA clone as hybridization probe did not show gross differences between the restriction patterns of the DNA obtained from the apo-E-deficient patient and two normal controls. When normal M-M cultures were grown in media containing [35S]methionine they synthesized and secreted apo-E into the culture media. In contrast we could not detect any intracellular or extracellular apo-E in the patient's M-M cultures grown under identical conditions. These observations are consistent with the hypothesis that familial apo-E deficiency results from structural apo-E gene mutation(s). The putative mutation(s) affect either the transcription of the apo-E gene or the processing of the primary apo-E mRNA transcript. These abnormalities are associated with low levels of synthesis of aberrant apo-E mRNA forms which are either very unstable or cannot be translated into protein.
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Cites
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245 · see citing works
Rigby, P. W. J., Dieckmann, M., Rhodes, C., & Berg, P. R. (1977) · Journal of Molecular Biology · doi:10.1016/0022-2836(77)90052-3 · Cited by 11,400 · see citing works
The Metabolic Basis of Inherited Disease
Solomon, L. M. (1979) · Journal of Investigative Dermatology · doi:10.1111/1523-1747.ep12541649 · Cited by 2,080 · see citing works
Fat Transport in Lipoproteins — An Integrated Approach to Mechanisms and Disorders
Fredrickson, D. S., Levy, R. I., & Lees, R. S. (1967) · New England Journal of Medicine · doi:10.1056/nejm196701052760107 · Cited by 1,911 · see citing works
Human apolipoprotein E. The complete amino acid sequence
Rall, S. C., Weisgraber, K. H., & Mahley, R. W. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34702-1 · Cited by 741 · see citing works
Polymorphism of apolipoprotein E and occurrence of dysbetalipoproteinaemia in man
UTERMANN, G., HEES, M., & STEINMETZ, A. (1977) · Nature · doi:10.1038/269604a0 · Cited by 666 · see citing works
Sniderman, A. D., Shapiro, S., Marpole, D. G. F., Skinner, B., Teng, B., & Kwiterovich, P. O. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.1.604 · Cited by 632 · see citing works
Zannis, V. I., & Breslow, J. L. (1981) · Biochemistry · doi:10.1021/bi00507a059 · Cited by 563 · see citing works
Self-citationRelative contributions by liver and intestine to individual plasma apolipoproteins in the rat.
Wu, A. L., & Windmueller, H. G. (1979) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)50321-5 · Cited by 446 · see citing works
Polymorphism of apolipoprotein E
Utermann, G., Vogelberg, K. H., Steinmetz, A., Schoenborn, W., Pruin, N., Jaeschke, M., Hees, M. T. I. W. S.-V., & Canzler, H. (1979) · Clinical Genetics · doi:10.1111/j.1399-0004.1979.tb02027.x · Cited by 408 · see citing works
THE MUTATION AND POLYMORPHISM OF THE HUMAN β-GLOBIN GENE AND ITS SURROUNDING DNA
Orkin, S. H., & Kazazian, H. H. (1984) · Annual Review of Genetics · doi:10.1146/annurev.ge.18.120184.001023 · Cited by 394 · see citing works
Lipoprotein binding to canine hepatic membranes. Metabolically distinct apo-E and apo-B,E receptors.
Hui, D. Y., Innerarity, T. L., & Mahley, R. W. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)69254-9 · Cited by 383 · see citing works
Isolation, characterization, and mapping to chromosome 19 of the human apolipoprotein E gene.
Das, H. K., McPherson, J. D., Bruns, G. A. P., Karathanasis, S. K., & Breslow, J. L. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)88963-3 · Cited by 351 · see citing works
Rall, S. C., Weisgraber, K. H., Innerarity, T. L., & Mahley, R. W. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.15.4696 · Cited by 345 · see citing works
Schneider, W. J., Kovanen, P. T., Brown, M. S., Goldstein, J. L., Utermann, G., Weber, W., Havel, R. J., Kotite, L., Kane, J. P., Innerarity, T. L., et al. (1981) · Journal of Clinical Investigation · doi:10.1172/jci110330 · Cited by 310 · see citing works
Nucleotide sequence and structure of the human apolipoprotein E gene.
Paik, Y. K., Chang, D. J., Reardon, C. A., Davies, G. E., Mahley, R. W., & Taylor, J. M. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.10.3445 · Cited by 281 · see citing works
Type III Hyperlipoproteinemia Associated with Apolipoprotein E Deficiency
Ghiselli, G., Schaefer, E. J., Gascon, P., & Breser, H. B. (1981) · Science · doi:10.1126/science.6795720 · Cited by 274 · see citing works
Self-citationPSEUDOMEMBRANOUS COLITIS ASSOCIATED WITH CLINDAMYCIN
Steer, H. W. (1974) · The Lancet · doi:10.1016/s0140-6736(74)90673-4 · Cited by 270 · see citing works
The biochemical, clinical, and genetic features of type III hyperlipoproteinemia.
MORGANROTH, J., LEVY, R. I., & FREDRICKSON, D. S. (1975) · Annals of Internal Medicine · doi:10.7326/0003-4819-82-2-158 · Cited by 260 · see citing works
Pitas, R. E., Innerarity, T. L., Arnold, K. S., & Mahley, R. W. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.5.2311 · Cited by 213 · see citing works
Utermann, G., Pruin, N., & Steinmetz, A. (1979) · Clinical Genetics · doi:10.1111/j.1399-0004.1979.tb02028.x · Cited by 209 · see citing works
Familial hyper-alpha-lipoproteinemia: Studies in eighteen kindreds
Glueck, C. J., Fallat, R. W., Millett, F., Gartside, P. S., Elston, R. C., & Go, R. C. P. (1975) · Metabolism · doi:10.1016/0026-0495(75)90063-3 · Cited by 205 · see citing works
Zannis, V. I., Cole, F. S., Jackson, C. L., Kurnit, D. M., & Karathanasis, S. K. (1985) · Biochemistry · doi:10.1021/bi00337a028 · Cited by 203 · see citing works
Self-citationbeta-Thalassemia in American Blacks: novel mutations in the "TATA" box and an acceptor splice site.
Antonarakis, S. E., Irkin, S. H., Cheng, T. C., Scott, A. F., Sexton, J. P., Trusko, S. P., Charache, S., & Kazazian, H. H. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.4.1154 · Cited by 191 · see citing works
Synthesis, intracellular processing, and signal peptide of human apolipoprotein E.
Zannis, V. I., McPherson, J. D., Goldberger, G., Karathanasis, S. K., & Breslow, J. L. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91039-2 · Cited by 189 · see citing works
Self-citationIdentification of a Nondeletion Defect in α-Thalassemia
Kan, Y. W., Dozy, A. M., Trecartin, R. F., & Todd, D. (1977) · New England Journal of Medicine · doi:10.1056/nejm197711172972002 · Cited by 188 · see citing works
Havel, R. J., Kotite, L., Vigne, J. L., Kane, J. P., Tun, P., PHILLIPS, N. R., & Chen, G. C. (1980) · Journal of Clinical Investigation · doi:10.1172/jci109988 · Cited by 184 · see citing works
Type III Hyperlipoproteinemia: Defective Metabolism of an Abnormal Apolipoprotein E
Gregg, R. E., Zech, L. A., Schaefer, E. J., & Brewer, H. B. (1981) · Science · doi:10.1126/science.7455696 · Cited by 180 · see citing works
Self-citationInnerarity, T. L., Weisgraber, K. H., Arnold, K. S., Rall, S. C., & Mahley, R. W. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)39866-6 · Cited by 179 · see citing works
Type III Hyperlipoproteinemia: An Analysis of Two Contemporary Definitions
Fredrickson, D. S., MORGANROTH, J., & LEVY, R. I. (1975) · Annals of Internal Medicine · doi:10.7326/0003-4819-82-2-150 · Cited by 177 · see citing works
Studies of familial type III hyperlipoproteinemia using as a genetic marker the apoE phenotype E2/2
Breslow, J. L., Zannis, V. I., SanGiacomo, T. R., Third, J. L., Tracy, T., & Glueck, C. J. (1982) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38060-3 · Cited by 174 · see citing works
Self-citationSynthesis of the second component of complement by long-term primary cultures of human monocytes.
Einstein, L. P., Schneeberger, E. E., & Colten, H. R. (1976) · The Journal of Experimental Medicine · doi:10.1084/jem.143.1.114 · Cited by 170 · see citing works
Quantitation of apolipoprotein E mRNA in the liver and peripheral tissues of nonhuman primates.
Newman, T. C., Dawson, P. A., Rudel, L. L., & Williams, D. L. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89575-8 · Cited by 145 · see citing works
Rall, S. C., Weisgraber, K. H., Innerarity, T. L., Bersot, T. P., Mahley, R. W., & Blum, C. B. (1983) · Journal of Clinical Investigation · doi:10.1172/jci111085 · Cited by 141 · see citing works
Hazzard, W. R., Porte, D., & Bierman, E. L. (1972) · Metabolism · doi:10.1016/0026-0495(72)90031-5 · Cited by 134 · see citing works
Failure of blood cardioplegia to protect myocardium at lower temperatures.
Gj, M., Flaherty, J. T., Vl, G., Bulkley, B. H., & Gardner, T. J. (1982) · PubMed · Cited by 113 · see citing works
Weisgraber, K. H., Rall, S. C., Innerarity, T. L., Mahley, R. W., Kuusi, T., & Ehnholm, C. (1984) · Journal of Clinical Investigation · doi:10.1172/jci111287 · Cited by 111 · see citing works
Schwartz, R. J., Haron, J. A., Rothblum, K. N., & Dugaiczyk, A. (1980) · Biochemistry · doi:10.1021/bi00566a034 · Cited by 101 · see citing works
Familial hypo-β-lipoproteinemia
Mars, H., Lewis, L. A., Robertson, A. L., Butkus, A., & Williams, G. H. (1969) · The American Journal of Medicine · doi:10.1016/0002-9343(69)90089-8 · Cited by 96 · see citing works
Kinetic studies of calcium binding to regulatory complexes from skeletal muscle.
Rosenfeld, S. S., & Taylor, E. W. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)89724-1 · Cited by 86 · see citing works
TYPE-III HYPERLIPOPROTEINÆMIA ("REMNANT REMOVAL DISEASE")
Chait, A., Albers, J. J., Brunzell, J. D., & Hazzard, W. R. (1977) · The Lancet · doi:10.1016/s0140-6736(77)92717-9 · Cited by 85 · see citing works
Hazzard, W. R., Warnick, G. R., Utermann, G., & Albers, J. J. (1981) · Metabolism · doi:10.1016/0026-0495(81)90223-7 · Cited by 77 · see citing works
Morrison, J. A., Kelly, K. A., Horvitz, R. A., Khoury, P. R., Laskarewski, P. M., Mellies, M. J., & Glueck, C. J. (1982) · Metabolism · doi:10.1016/0026-0495(82)90129-9 · Cited by 67 · see citing works
Broad-β Disease (Type III Hyperlipoproteinemia) in a Large Kindred
Hazzard, W. R., O'DONNELL, T. F., & LEE, Y. L. (1975) · Annals of Internal Medicine · doi:10.7326/0003-4819-82-2-141 · Cited by 50 · see citing works
A longitudinal assessment of lipid ratios in the diagnosis of type III hyperlipoproteinaemia
Mishkel, M. A., Nazir, D. J., & Crowther, S. M. (1975) · Clinica Chimica Acta · doi:10.1016/s0009-8981(75)80004-0 · Cited by 49 · see citing works
Single-stranded poly(deoxyguanylic acid) associates into double- and triple-stranded structures
Dugaiczyk, A., Robberson, D. L., & Ullrich, A. (1980) · Biochemistry · doi:10.1021/bi00566a032 · Cited by 21 · see citing works
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Ishii, M., Hashimoto, S., Tsutsumi, S., Wada, Y., Matsushima, K., Kodama, T., & Aburatani, H. (2000) · Genomics · doi:10.1006/geno.2000.6284 · Cited by 206 · see citing works
Lin, C., Xu, Y., Wu, J., & Chan, L. (1986) · Journal of Clinical Investigation · doi:10.1172/jci112685 · Cited by 115 · see citing works
Cladaras, C., Hadzopoulou-Cladaras, M., Felber, B. K., Pavlakis, G., & Zannis, V. I. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61655-2 · Cited by 92 · see citing works
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Zannis, V. I., Kardassis, D., & Zanni, E. E. (1993) · Advances in Human Genetics 21 · doi:10.1007/978-1-4615-3010-7_3 · Cited by 78 · see citing works
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Mabuchi, H., Itoh, H., Takeda, M., Kajinami, K., Wakasugi, T., Koizumi, J., Takeda, R., & Asagami, C. (1989) · Metabolism · doi:10.1016/0026-0495(89)90249-7 · Cited by 70 · see citing works
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Hegele, R. A., Breslow, J. L., & Cloninger, C. R. (1987) · Genetic Epidemiology · doi:10.1002/gepi.1370040302 · Cited by 60 · see citing works
Lohse, P., Brewer, H., Meng, M., Skarlatos, S., LaRosa, J., & Brewer, H. (1992) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)41380-x · Cited by 59 · see citing works
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Self-citation
- Authors
- Zannis, V. I., Ordovas, J. M., Cladaras, C., Cole, F. S., Forbes, G., & Schaefer, E. J.
- Affiliations
- Boston University (US)
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1985-10
- Volume
- 260
- Issue
- 24
- Pages
- 12891-12894
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1533930495
- Cited by
- 21
- References
- 46
- FWCI
- 3.15
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL-33952)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Keywords
- apolipoprotein E deficiency, monocytes/macrophages, mRNA processing, genetic mutations, transcription, protein synthesis, mRNA instability, actin mRNA
- MeSH terms
- Apolipoproteins E, Cells, Cultured, DNA, DNA Restriction Enzymes, Humans, Macrophages, Monocytes, Mutation, Nucleic Acid Hybridization, RNA, Messenger, Transcription, Genetic
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